... metabolism. Methods: Mice underwent unilateral hypoxia-ischemia (HI) on postnatal day (P)10. Injured and sham mice were scanned at P10, P17, and P31. We used hyperpolarized 13 C MRS to investigate the metabolic exchange of pyruvate to lactate in real time during brain development following HI. 13 C-1-labeled...

...) would increase the rate of metabolism of glutamate, pyruvate was added in some experiments. Addition of 1 m M pyruvate increased the rate of oxidation of glutamate, and the increase was inhibited by AOAA, consistent with enhanced entry of glutamate into the TCA cycle via transamination in the presence...

... Pyruvate Protein oxidation Mitochondria Rat brain Pediatric traumatic brain injury National Institutes of Health (NIH) 10.13039/100000002 Basic Science Research Dev Neurosci 2006;28:410 419 DOI: 10.1159/000094167 Received: December 13, 2005 Accepted: April 4, 2006 Gel-Based Hippocampal...

...-mediated uptake mechanisms with a high-affinity K m of 0.66 m M and V max of 3.66 m M for pyruvate, and a low-affinity system with a K m of 9.9 m M for both lactate and pyruvate and V max values of 16.6 and 23.1 nmol/30 s/mg protein for lactate and pyruvate, respectively. Saturable uptake was seen...

... plus L-alanine function as the transmitter precursors. The key reason that an interaction with astrocytes is essential is that both pyruvate carboxylase, the major enzyme in the brain for net synthesis of tricarboxylic acid cycle intermediates, and glutamine synthetase, the enzyme forming glutamine...