..., instructions or products referred to in the content or advertisements. 2023 Neonatal Erythropoietin Neuroprotection MRI Lactate NIH Neonatal hypoxic-ischemic encephalopathy (HIE) remains a leading cause of death and long-term neurodevelopmental disability worldwide [ 1 ]. While...

... metabolism. Methods: Mice underwent unilateral hypoxia-ischemia (HI) on postnatal day (P)10. Injured and sham mice were scanned at P10, P17, and P31. We used hyperpolarized 13 C MRS to investigate the metabolic exchange of pyruvate to lactate in real time during brain development following HI. 13 C-1-labeled...

...Alkisti Mikrogeorgiou; Duan Xu; Donna M. Ferriero; Susan J. Vannucci Brain development is an energy-expensive process. Although glucose is irreplaceable, the developing brain utilizes a variety of substrates such as lactate and the ketone bodies, β-hydroxybutyrate and acetoacetate, to produce...

... the rates of 14 CO 2 production from 1 mM D-[6- 14 C]glucose, L-[U- 14 C]glutamine, D-3-hydroxy[3- 14 C]butyrate, L-[U- 14 C]lactate and L-[U- 14 C]malate by synaptic terminals isolated from 17- to 18-day-old and 7- to 8-week-old rat brain. The rates of 14 CO 2 production from glucose, glutamine, 3...

...Jakob Korf We developed techniques to continuously monitor lactate in the living rat ('lactography') based on microdialysis and on-line enzymatic conversion of lactate in the dialysate using either continuous flow technologies or enzyme reactors. In vivo lactate was monitored during a single...

...Raymond A. Swanson; Joel H. Benington Astrocytes in primary culture produce lactate. The net production of lactate from glucose requires that the carbon flux through glycolysis exceed the carbon flux to CO 2 . This study investigates the control and function of this 'excess' glycolysis in astrocyte...

..., astrocytes and cocultures thereof during normoxia and hypoxia. Lactate dehydrogenase leakage was used to monitor the cytoprotective effects of IGF-I and insulin. Thus, during normoxia both peptides decreased LDH leakage from neurons. During hypoxia, however, this protection was only observed when insulin...

... is processed glycolytically thus leading to increased lactate production. Since excitatory amino acids are released during activation by cortical afferents, these data reveal a simple mechanism for coupling neuronal activity to glucose utilization and provide further evidence for the concept of a transient...

... in the sensitizing effects of LPS and lipoteichoic acid (LTA) in combination with HI in 7-day-old rats. LPS/HI resulted in hypoglycemia which lasted 24 h and lactate levels were increased from 6 to 10 h after LPS administration. LPS/HI induced severe brain injury, which persisted 2 weeks after LPS/HI. Administration...

...Hong Qu; Asta Håberg; Olav Haraldseth; Geirmund Unsgård; Ursula Sonnewald In order to address the question whether lactate in blood can serve as a precursor for cerebral metabolites, fully awake rats were injected intravenously with [U- 13 C]lactate or [U- 13 C]glucose followed 15 min later...

... the cells in media containing 0.5 m M [U- 13 C]glucose in the absence or presence of glutamine (0.5 m M ). Lyophilized cell extracts were analyzed by 13 C nuclear magnetic resonance (NMR) spectroscopy and HPLC. The present findings were compared to results previously obtained using 1.0 m M [U- 13 C]lactate...

...A.-K. Bouzier; P. Voisin; R. Goodwin; P. Canioni; M. Merle 13 C and 1 H nuclear magnetic resonance spectroscopy (NMR) was used to investigate the metabolism of L -lactate and D -glucose in C6 glioma cells. The 13 C enrichment of cell metabolites was examined after a 4-h incubation in media...

...Mary C. McKenna; J. Tyson Tildon; Joseph H. Stevenson; Irene B. Hopkins; Xueli Huang; Renee Couto Since lactate released by glial cells may be a key substrate for energy in neurons, the kinetics for the uptake of L -[U- 14 C]lactate by cortical synaptic terminals from 7- to 8-week-old rat brain...

... electrode; local cerebral blood flow (rCBF) at an implanted platinum electrode using the hydrogen clearance technique; extracellular brain glucose at an implanted glucose oxidase-based biosensor and changes in lactate were measured using microdialysis. The nitrogen/air mixture led to a decrease in tissue...

... that is released by astrocytes is predominantly lactate and not glucose. Since lactate can support neuronal activity and synaptic function in vitro, the possibility should be considered that glucose uptake by the brain parenchyma occurs predominantly into astrocytes which subsequently release lactate for the use...

... into the medium by the astrocyte cultures and the mixed astroglial/neuronal cultures, measurable amounts of label were present in lactate C-3 and C-2, glutamine C-2, C-3 and C-4, acetate C-2, citrate C-2 or C-4 and C-3, glycerol C-1 or C-3, succinate C-2 or C-3 and several unidentified metabolites. Of the labeled...

... synaptic terminals from rat brain. The rates of 14 CO 2 production from labelled substrates by astrocytes were 0.96 ± 0.13, 11.13 ± 0.67, 10.51 ± 0.35, 24.92 ± 1.66 and 4.80 ± 0.50 for D -[6- 14 C]gIucose, L -[U- 14 C]lactate, D -3-hydroxy[3- 14 C]butyrate, L -[U- 14 C]gIutamine and L -[U- 14 C]ma-late...