...Alkisti Mikrogeorgiou; Duan Xu; Donna M. Ferriero; Susan J. Vannucci Brain development is an energy-expensive process. Although glucose is irreplaceable, the developing brain utilizes a variety of substrates such as lactate and the ketone bodies, β-hydroxybutyrate and acetoacetate, to produce...

... the rates of 14 CO 2 production from 1 mM D-[6- 14 C]glucose, L-[U- 14 C]glutamine, D-3-hydroxy[3- 14 C]butyrate, L-[U- 14 C]lactate and L-[U- 14 C]malate by synaptic terminals isolated from 17- to 18-day-old and 7- to 8-week-old rat brain. The rates of 14 CO 2 production from glucose, glutamine, 3...

...Astrid Nehlig The postnatal evolution of local cerebral metabolic rates for glucose (LCM-Rglcs) and of regional rates of cerebral uptake of β-hydroxybutyrate (βHB) were studied in the suckling rat between postnatal days (P) 10 and 21. LCM-Rglcs were low and homogeneous at P10. They increased...

...Rob Forsyth; Ann Fray; Martyn Boutelle; Marianne Fillenz; Claire Middleditch; Ann Burchell The present paper examines the possible role of astrocytes in the delivery of glycogen-derived glucose for neuronal metabolism. Such a process would require astrocytic expression of glucose-6-phosphatase...

...Robert W. Flint Jr.; Dominick Papandrea Jr.; Nancy Dorr Previous research has shown that developmentally related memory impairments in immature rodents are malleable and may be attenuated using a variety of pharmacological and behavioral treatments. This experiment examined the effects of glucose...

...Hong Qu; Asta Håberg; Olav Haraldseth; Geirmund Unsgård; Ursula Sonnewald In order to address the question whether lactate in blood can serve as a precursor for cerebral metabolites, fully awake rats were injected intravenously with [U- 13 C]lactate or [U- 13 C]glucose followed 15 min later...

...Susan J. Vannucci; Tina Rutherford; Mary Beth Wilkie; Ian A. Simpson; Jean M. Lauder The GLUT4 glucose transporter is primarily expressed in skeletal muscle, heart and adipose tissue, where its expression is postnatal, coincident with the acquisition of insulin-regulated glucose transport...

...A.-K. Bouzier; P. Voisin; R. Goodwin; P. Canioni; M. Merle 13 C and 1 H nuclear magnetic resonance spectroscopy (NMR) was used to investigate the metabolism of L -lactate and D -glucose in C6 glioma cells. The 13 C enrichment of cell metabolites was examined after a 4-h incubation in media...

... the cells in media containing 0.5 m M [U- 13 C]glucose in the absence or presence of glutamine (0.5 m M ). Lyophilized cell extracts were analyzed by 13 C nuclear magnetic resonance (NMR) spectroscopy and HPLC. The present findings were compared to results previously obtained using 1.0 m M [U- 13 C]lactate...

... neurons and astrocytes is revealed which suggests that a population of astrocytes is a lactate ‘source’ while neurons may be a lactate ‘sink’. Third, we provide biochemical evidence that lactate is interchangeable with glucose to support oxidative metabolism in cortical neurons. This set of data...

... electrode; local cerebral blood flow (rCBF) at an implanted platinum electrode using the hydrogen clearance technique; extracellular brain glucose at an implanted glucose oxidase-based biosensor and changes in lactate were measured using microdialysis. The nitrogen/air mixture led to a decrease in tissue...

...Yinyin Huang; Carol L. Zielke; Tyson Tildon; H. Ronald Zielke Cultured brain cells oxidize glucose and numerous alternate substrates to CO 2 for energy production, however, the importance of these observations to the intact brain have not been established. We have adapted in vivo brain...

...Jakob Korf; Jaep de Boer; Rienk Baarsma; Kor Venema; Albert Okken Methods for the continuous in vivo monitoring of glucose and lactate were applied in man for studies of peripheral metabolism and, in rats, for cerebral examinations. Lactate (''lactography'') and glucose were continuously measured...

... toward the activity in neuropil. Studies of local glucose utilization (lCMR glc ) indicate that energy metabolism is increased by functional activation mainly in terminal projection zones of activated pathways. Electrical stimulation of a pathway raises lCMR glc in the projection zones of the pathway...

... of glutamine oxidation 5-fold, consistent with other studies showing that glutamine enters the TCA cycle via transamination. AOAA increased the rate of 14 CO 2 production from labelled glucose 4-fold, suggesting that inhibition of alanine biosynthesis profoundly alters the utilization of glucose by astrocytes...