... proliferation was inhibited. The optimal conditions for the production of a predominantly neuronal culture were to use cerebral hemispheres from 7-day-old chick embryos, to dissociate the brain tissue mechanically and to seed the cells at a concentration range between 1.5 and 5 × 10 6 cells/ml. The cultures...

... the influence of bFGF. Treatment with bFGF after the 2nd day of culture had no or only a low stimulatory effect. Our findings indicate that bFGF affects the development of AChE-containing neurons, i.e. cholinoceptive neurons from rat cerebrum. Original Paper Dev Ncurosci 1992;14:278-281 C. Gensburger3 M. Capob...

... and GABA metabolism were studied throughout neuronal development in culture. The presence of GABAergic neurons in the cultured cell population was demonstrated. There were time-dependent changes in cellular polyamine concentrations and the activities of the enzymes involved in polyamine metabolism. Since...

... this differentiated state independently of tetrodotoxin-blocked muscle activity. Dev. Neurosci. 5 :314-325 (1982) Establishment of Neuromuscular Contacts in Cultures of Rat Embryonic Cells: Effect of Tetrodotoxin on Maturation of Muscle Fibers and on Formation and Maintenance of Acetylcholinesterase andAcetylcholine...

...Nadejda Andreeva; Julia Heldt; Norbert Leclere; Johann Gross The effect of hypoxia on immature and mature mesencephalic neurons was studied in in vitro rat cerebral cell cultures on different days. In immature cultures (6–8 days in vitro), exposure to 24 h of hypoxia (10–20 mm Hg pO 2...

...Monique Miehe; Jean-François Leterrier; Jean-Christophe Deloulme; Claire Gensburger; Marie-France Knoetgen; Monique Sensenbrenner We have examined the ultrastructural aspect of neuronal precursors derived from 14-day-old rat embryos during their development under various culture conditions. Cells...

...Linda L. Bambrick; Peter E. Braun The myelin-associated glycoprotein (MAG) in primary cultures of oligodendrocytes is subject to phosphorylation in the absence of neurons. The positive response of this phosphorylation to vanadate suggests that one of the modified sites in MAG is phosphotyrosine. We...

...Isabelle Lelong; Eric Frechard; Gérard Cremel; Keith Langley; Gérard Rebel; Guy Vincendon; Marcel Mersel Plasma membranes isolated from chick embryo neurons after 2 days (division phase) and 5 days (maturation phase) in culture showed no differences in the phospholipid (PL) pattern or in the plasma...

... (DRG) cells in the chick embryo was tested in vitro in coculture with myoblasts, in conditioned medium (CM) prepared from myoblasts and in control cultures of DRG cells alone. Control cultures of DRG cells grown at the 6 th embryonic day (E6) did not show any calbindin-immunostained ganglion cell...

... cord-ganglion synapses since their blockade with the α-bungarotoxin-horseradish peroxidase complex did not prevent synapse formation in culture. The presence of acetylcholine receptors appears to be sufficient for synapse formation since inappropriate motoneurone-ganglion synapses could form and were...