The endocannabinoid system (ECS) is widely studied due to its interactions with cannabis and its role in modulating physiological responses. While most research has focused on the effects of cannabis on adult ECSs, recent studies have begun to investigate the role of the ECS in developing organisms. However, little is known about the spatial or temporal expression of these receptors during early development. This study combines reverse transcriptase-polymerase chain reaction (RT-PCR) with in situ hybridizations to compile a timeline of the developmental expression of six key cannabinoid receptors; cb1, cb2, trpv1, trpa1a, trpa1b, and gpr55 in zebrafish embryos, starting from as early as 6-h postfertilization (hpf) until 3 days pf (dpf). This time frame is roughly equivalent to 2–10 weeks in human embryonic development. All six genes were confirmed to be expressed within this time range and share similarities with human and rodent expression. Cb1 expression was first detected between 12 and 24 hpf in the retina and CNS, and its expression increased thereafter and was more evident in the olfactory bulb, tegmentum, hypothalamus, and gut. Cb2 expression was relatively high at the 6 and 24 hpf timepoints, as determined by RT-PCR but was undetectable at other times. Trpv1 was first detected at 1 dpf in the trigeminal (Tg) ganglia, Rohon-Beard neurons, and lateral line, and its expression increased in the first 3 dpf. Expression of trpa1a was first detected as late as 3 dpf in vagal (V) neurons, whereas trpa1b was first detected at 1 dpf associated with Tg, glossopharyngeal, and V ganglia. Expression of gpr55 was diffuse and widespread throughout the brain and head region but was undetectable elsewhere in the embryo. Thus, receptor expression was found to be enriched in the central nervous system and within sensory neurons. This work aims to serve as a foundation for further investigation on the role of cannabinoid and cannabinoid-interacting receptors in early embryonic development.

1.
Chevaleyre
V
,
Takahashi
KA
,
Castillo
PE
.
Endocannabinoid-mediated synaptic plasticity in the CNS
.
Annu Rev Neurosci
.
2006
;
29
:
37
76
. .
2.
Heifets
BD
,
Castillo
PE
.
Endocannabinoid signaling and long-term synaptic plasticity
.
Annu Rev Physiol
.
2009
;
71
:
283
306
. .
3.
Basavarajappa
BS
,
Shivakumar
M
,
Joshi
V
,
Subbanna
S
.
Endocannabinoid system in neurodegenerative disorders
.
J Neurochem
.
2017 Sep
;
142
(
5
):
624
48
. .
4.
Di Marzo
V
.
The endocannabinoidome as a substrate for noneuphoric phytocannabinoid action and gut microbiome dysfunction in neuropsychiatric disorders
.
Dialogues Clin Neurosci
.
2020 Sep
;
22
(
3
):
259
69
. .
5.
Oltrabella
F
,
Melgoza
A
,
Nguyen
B
,
Guo
S
.
Role of the endocannabinoid system in vertebrates: emphasis on the zebrafish model
.
Dev Growth Differ
.
2017 May
;
59
(
4
):
194
210
. .
6.
Pertwee
RG
.
Cannabinoid pharmacology: the first 66 years
.
Br J Pharmacol
.
2006 Jan
;
147
(
1
):
S163
71
. .
7.
Marsicano
G
,
Lutz
B
.
Expression of the cannabinoid receptor CB1 in distinct neuronal subpopulations in the adult mouse forebrain
.
Eur J Neurosci
.
1999 Dec
;
11
(
12
):
4213
25
. .
8.
Kawamura
Y
,
Fukaya
M
,
Maejima
T
,
Yoshida
T
,
Miura
E
,
Watanabe
M
,
The CB1 cannabinoid receptor is the major cannabinoid receptor at excitatory presynaptic sites in the hippocampus and cerebellum
.
J Neurosci
.
2006 Mar 15
;
26
(
11
):
2991
3001
.
9.
Van Waes
V
,
Beverley
JA
,
Siman
H
,
Tseng
KY
,
Steiner
H
.
CB1 cannabinoid receptor expression in the striatum: association with corticostriatal circuits and developmental regulation
.
Front Pharmacol
.
2012
;
3
:
21
. .
10.
Domenici
MR
,
Azad
SC
,
Marsicano
G
,
Schierloh
A
,
Wotjak
CT
,
Dodt
HU
,
Cannabinoid receptor type 1 located on presynaptic terminals of principal neurons in the forebrain controls glutamatergic synaptic transmission
.
J Neurosci
.
2006 May 24
;
26
(
21
):
5794
9
.
11.
Li
H
,
Yang
J
,
Tian
C
,
Diao
M
,
Wang
Q
,
Zhao
S
,
Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging
.
Nature commun
.
2020 Nov 11
;
11
(
1
):
5699
.
12.
Graham
ES
,
Angel
CE
,
Schwarcz
LE
,
Dunbar
PR
,
Glass
M
.
Detailed characterisation of CB2 receptor protein expression in peripheral blood immune cells from healthy human volunteers using flow cytometry
.
Int J Immunopathol Pharmacol
.
2010 Jan
;
23
(
1
):
25
34
. .
13.
Nunez
E
,
Benito
C
,
Pazos
MR
,
Barbachano
A
,
Fajardo
O
,
Gonzalez
S
,
Cannabinoid CB2 receptors are expressed by perivascular microglial cells in the human brain: an immunohistochemical study
.
Synapse
.
2004 Sep 15
;
53
(
4
):
208
13
.
14.
Eisenstein
TK
,
Meissler
JJ
,
Wilson
Q
,
Gaughan
JP
,
Adler
MW
.
Anandamide and Delta9-tetrahydrocannabinol directly inhibit cells of the immune system via CB2 receptors
.
J Neuroimmunol
.
2007 Sep
;
189
(
1–2
):
17
22
. .
15.
Svizenska
I
,
Dubovy
P
,
Sulcova
A
.
Cannabinoid receptors 1 and 2 (CB1 and CB2), their distribution, ligands and functional involvement in nervous system structures: a short review
.
Pharmacol Biochem Behav
.
2008 Oct
;
90
(
4
):
501
11
.
16.
Turcotte
C
,
Blanchet
MR
,
Laviolette
M
,
Flamand
N
.
The CB2 receptor and its role as a regulator of inflammation
.
Cell Mol Life Sci
.
2016 Dec
;
73
(
23
):
4449
70
. .
17.
Onaivi
ES
.
Neuropsychobiological evidence for the functional presence and expression of cannabinoid CB2 receptors in the brain
.
Neuropsychobiology
.
2006
;
54
(
4
):
231
46
. .
18.
Onaivi
ES
,
Ishiguro
H
,
Gong
JP
,
Patel
S
,
Meozzi
PA
,
Myers
L
,
Brain neuronal CB2 cannabinoid receptors in drug abuse and depression: from mice to human subjects
.
PLoS One
.
2008 Feb 20
;
3
(
2
):
e1640
.
19.
Onaivi
ES
,
Ishiguro
H
,
Gong
JP
,
Patel
S
,
Meozzi
PA
,
Myers
L
,
Functional expression of brain neuronal CB2 cannabinoid receptors are involved in the effects of drugs of abuse and in depression
.
Ann N Y Acad Sci
.
2008 Oct
;
1139
:
434
49
.
20.
Liu
QR
,
Pan
CH
,
Hishimoto
A
,
Li
CY
,
Xi
ZX
,
Llorente-Berzal
A
,
Species differences in cannabinoid receptor 2 (CNR2 gene): identification of novel human and rodent CB2 isoforms, differential tissue expression and regulation by cannabinoid receptor ligands
.
Genes Brain Behav
.
2009 Jul
;
8
(
5
):
519
30
.
21.
Stempel
AV
,
Stumpf
A
,
Zhang
HY
,
Ozdogan
T
,
Pannasch
U
,
Theis
AK
,
Cannabinoid type 2 receptors mediate a cell type-specific plasticity in the hippocampus
.
Neuron
.
2016 May 18
;
90
(
4
):
795
809
.
22.
Atwood
BK
,
Mackie
K
.
CB2: a cannabinoid receptor with an identity crisis
.
Br J Pharmacol
.
2010 Jun
;
160
(
3
):
467
79
. .
23.
Lanciego
JL
,
Barroso-Chinea
P
,
Rico
AJ
,
Conte-Perales
L
,
Callen
L
,
Roda
E
,
Expression of the mRNA coding the cannabinoid receptor 2 in the pallidal complex of Macaca fascicularis
.
J Psychopharmacol
.
2011 Jan
;
25
(
1
):
97
104
.
24.
Garcia-Gutierrez
MS
,
Garcia-Bueno
B
,
Zoppi
S
,
Leza
JC
,
Manzanares
J
.
Chronic blockade of cannabinoid CB2 receptors induces anxiolytic-like actions associated with alterations in GABA(A) receptors
.
Br J Pharmacol
.
2012 Feb
;
165
(
4
):
951
64
.
25.
Li
Y
,
Kim
J
.
Neuronal expression of CB2 cannabinoid receptor mRNAs in the mouse hippocampus
.
Neuroscience
.
2015 Dec 17
;
311
:
253
67
. .
26.
Van Sickle
MD
,
Duncan
M
,
Kingsley
PJ
,
Mouihate
A
,
Urbani
P
,
Mackie
K
,
Identification and functional characterization of brainstem cannabinoid CB2 receptors
.
Science
.
2005 Oct 14
;
310
(
5746
):
329
32
.
27.
Muller
C
,
Morales
P
,
Reggio
PH
.
Cannabinoid ligands targeting TRP channels
.
Front Mol Neurosci
.
2018
;
11
:
487
. .
28.
Storozhuk
MV
,
Zholos
AV
.
TRP channels as novel targets for endogenous ligands: focus on endocannabinoids and nociceptive signalling
.
Curr Neuropharmacol
.
2018 Jan 30
;
16
(
2
):
137
50
. .
29.
Kowalski
CW
,
Ragozzino
FJ
,
Lindberg
JEM
,
Peterson
B
,
Lugo
JM
,
McLaughlin
RJ
,
Cannabidiol activation of vagal afferent neurons requires TRPA1
.
J Neurophysiol
.
2020 Nov 1
;
124
(
5
):
1388
98
.
30.
Kano
M
.
Control of synaptic function by endocannabinoid-mediated retrograde signaling
.
Proc Jpn Acad Ser B Phys Biol Sci
.
2014
;
90
(
7
):
235
50
. .
31.
Starkus
J
,
Jansen
C
,
Shimoda
LMN
,
Stokes
AJ
,
Small-Howard
AL
,
Turner
H
.
Diverse TRPV1 responses to cannabinoids
.
Channels
.
2019 Dec
;
13
(
1
):
172
91
. .
32.
Wang
H
,
Siemens
J
.
TRP ion channels in thermosensation, thermoregulation and metabolism
.
Temperature
.
2015 Apr
;
2
(
2
):
178
87
. .
33.
Mickle
AD
,
Shepherd
AJ
,
Mohapatra
DP
.
Nociceptive TRP channels: sensory detectors and transducers in multiple pain pathologies
.
Pharmaceuticals
.
2016 Nov 14
;
9
(
4
):
72
. .
34.
Gau
P
,
Curtright
A
,
Condon
L
,
Raible
DW
,
Dhaka
A
.
An ancient neurotrophin receptor code; a single Runx/Cbfβ complex determines somatosensory neuron fate specification in zebrafish
.
PLoS Genet
.
2017 Jul
;
13
(
7
):
e1006884
. .
35.
Dux
M
,
Rosta
J
,
Messlinger
K
.
TRP channels in the focus of trigeminal nociceptor sensitization contributing to primary headaches
.
Int J Mol Sci
.
2020 Jan 4
;
21
(
1
):
342
. .
36.
Bisogno
T
,
Hanus
L
,
De Petrocellis
L
,
Tchilibon
S
,
Ponde
DE
,
Brandi
I
,
Molecular targets for cannabidiol and its synthetic analogues: effect on vanilloid VR1 receptors and on the cellular uptake and enzymatic hydrolysis of anandamide
.
Br J Pharmacol
.
2001 Oct
;
134
(
4
):
845
52
.
37.
Costa
B
,
Giagnoni
G
,
Franke
C
,
Trovato
AE
,
Colleoni
M
.
Vanilloid TRPV1 receptor mediates the antihyperalgesic effect of the nonpsychoactive cannabinoid, cannabidiol, in a rat model of acute inflammation
.
Br J Pharmacol
.
2004 Sep
;
143
(
2
):
247
50
. .
38.
Iannotti
FA
,
Hill
CL
,
Leo
A
,
Alhusaini
A
,
Soubrane
C
,
Mazzarella
E
,
Nonpsychotropic plant cannabinoids, cannabidivarin (CBDV) and cannabidiol (CBD), activate and desensitize transient receptor potential vanilloid 1 (TRPV1) channels in vitro: potential for the treatment of neuronal hyperexcitability
.
ACS Chem Neurosci
.
2014 Nov 19
;
5
(
11
):
1131
41
.
39.
Sawzdargo
M
,
Nguyen
T
,
Lee
DK
,
Lynch
KR
,
Cheng
R
,
Heng
HH
,
Identification and cloning of three novel human G protein-coupled receptor genes GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain
.
Brain Res Mol Brain Res.
1999 Feb 5
;
64
(
2
):
193
8
.
40.
Baker
D
,
Pryce
G
,
Davies
WL
,
Hiley
CR
.
In silico patent searching reveals a new cannabinoid receptor
.
Trends Pharmacol Sci
.
2006 Jan
;
27
(
1
):
1
4
. .
41.
Lauckner
JE
,
Jensen
JB
,
Chen
HY
,
Lu
HC
,
Hille
B
,
Mackie
K
.
GPR55 is a cannabinoid receptor that increases intracellular calcium and inhibits M current
.
Proc Natl Acad Sci U S A
.
2008 Feb 19
;
105
(
7
):
2699
704
. .
42.
Ryberg
E
,
Larsson
N
,
Sjogren
S
,
Hjorth
S
,
Hermansson
NO
,
Leonova
J
,
The orphan receptor GPR55 is a novel cannabinoid receptor
.
Br J Pharmacol
.
2007 Dec
;
152
(
7
):
1092
101
.
43.
Marichal-Cancino
BA
,
Fajardo-Valdez
A
,
Ruiz-Contreras
AE
,
Mendez-Díaz
M
,
Prospero-García
O
.
Advances in the physiology of GPR55 in the central nervous system
.
Curr Neuropharmacol
.
2017
;
15
(
5
):
771
8
. .
44.
Panula
P
,
Chen
YC
,
Priyadarshini
M
,
Kudo
H
,
Semenova
S
,
Sundvik
M
,
The comparative neuroanatomy and neurochemistry of zebrafish CNS systems of relevance to human neuropsychiatric diseases
.
Neurobiol Dis
.
2010 Oct
;
40
(
1
):
46
57
.
45.
Du
Y
,
Guo
Q
,
Shan
M
,
Wu
Y
,
Huang
S
,
Zhao
H
,
Spatial and temporal distribution of dopaminergic neurons during development in zebrafish
.
Front Neuroanat
.
2016
;
10
:
115
.
46.
Lam
CS
,
Rastegar
S
,
Strähle
U
.
Distribution of cannabinoid receptor 1 in the CNS of zebrafish
.
Neuroscience
.
2006
;
138
(
1
):
83
95
. .
47.
Rodriguez-Martin
I
,
Herrero-Turrion
MJ
,
Marron Fdez de Velasco
E
,
Gonzalez-Sarmiento
R
,
Rodriguez
RE
.
Characterization of two duplicate zebrafish Cb2-like cannabinoid receptors
.
Gene
.
2007 Mar 1
;
389
(
1
):
36
44
. .
48.
Thisse
C
,
Thisse
B
.
High-resolution in situ hybridization to whole-mount zebrafish embryos
.
Nat Protoc
.
2008
;
3
(
1
):
59
69
. .
49.
Liu
LY
,
Alexa
K
,
Cortes
M
,
Schatzman-Bone
S
,
Kim
AJ
,
Mukhopadhyay
B
,
Cannabinoid receptor signaling regulates liver development and metabolism
.
Development
.
2016 Feb 15
;
143
(
4
):
609
22
.
50.
Colon-Cruz
L
,
Rodriguez-Morales
R
,
Santana-Cruz
A
,
Cantres-Velez
J
,
Torrado-Tapias
A
,
Lin
SJ
,
Cnr2 is important for ribbon synapse maturation and function in hair cells and photoreceptors
.
Front Mol Neurosci
.
2021
;
14
:
624265
.
51.
Gau
P
,
Poon
J
,
Ufret-Vincenty
C
,
Snelson
CD
,
Gordon
SE
,
Raible
DW
,
The zebrafish ortholog of TRPV1 is required for heat-induced locomotion
.
J Neurosci
.
2013 Mar 20
;
33
(
12
):
5249
60
.
52.
Reyes
R
,
Haendel
M
,
Grant
D
,
Melancon
E
,
Eisen
JS
.
Slow degeneration of zebrafish Rohon-Beard neurons during programmed cell death
.
Dev Dyn
.
2004 Jan
;
229
(
1
):
30
41
. .
53.
Slatter
CA
,
Kanji
H
,
Coutts
CA
,
Ali
DW
.
Expression of PKC in the developing zebrafish, Danio rerio
.
J Neurobiol
.
2005 Mar
;
62
(
4
):
425
38
. .
54.
Prober
DA
,
Zimmerman
S
,
Myers
BR
,
McDermott
BM
 Jr
,
Kim
SH
,
Caron
S
,
Zebrafish TRPA1 channels are required for chemosensation but not for thermosensation or mechanosensory hair cell function
.
J Neurosci
.
2008 Oct 1
;
28
(
40
):
10102
10
.
55.
Straiker
AJ
,
Maguire
G
,
Mackie
K
,
Lindsey
J
.
Localization of cannabinoid CB1 receptors in the human anterior eye and retina
.
Invest Ophthalmol Vis Sci
.
1999 Sep
;
40
(
10
):
2442
8
.
56.
Porcella
A
,
Maxia
C
,
Gessa
GL
,
Pani
L
.
The human eye expresses high levels of CB1 cannabinoid receptor mRNA and protein
.
Eur J Neurosci
.
2000 Mar
;
12
(
3
):
1123
7
. .
57.
Lalonde
MR
,
Jollimore
CA
,
Stevens
K
,
Barnes
S
,
Kelly
ME
.
Cannabinoid receptor-mediated inhibition of calcium signaling in rat retinal ganglion cells
.
Mol Vis
.
2006 Oct 6
;
12
:
1160
6
.
58.
Zabouri
N
,
Bouchard
JF
,
Casanova
C
.
Cannabinoid receptor type 1 expression during postnatal development of the rat retina
.
J Comp Neurol
.
2011 May 1
;
519
(
7
):
1258
80
. .
59.
Cecyre
B
,
Zabouri
N
,
Huppe-Gourgues
F
,
Bouchard
JF
,
Casanova
C
.
Roles of cannabinoid receptors type 1 and 2 on the retinal function of adult mice
.
Invest Ophthalmol Vis Sci
.
2013 Dec 11
;
54
(
13
):
8079
90
.
60.
Yazulla
S
,
Studholme
KM
,
McIntosh
HH
,
Fan
SF
.
Cannabinoid receptors on goldfish retinal bipolar cells: electron-microscope immunocytochemistry and whole-cell recordings
.
Vis Neurosci
.
2000 May
;
17
(
3
):
391
401
. .
61.
Onaivi
ES
,
Ishiguro
H
,
Gong
JP
,
Patel
S
,
Perchuk
A
,
Meozzi
PA
,
Discovery of the presence and functional expression of cannabinoid CB2 receptors in brain
.
Ann N Y Acad Sci
.
2006
;
1074
:
514
36
.
62.
Zurolo
E
,
Iyer
AM
,
Spliet
WG
,
Van Rijen
PC
,
Troost
D
,
Gorter
JA
,
CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies
.
Neuroscience
.
2010
;
170
(
1
):
28
41
.
63.
Buckley
NE
,
Hansson
S
,
Harta
G
,
Mezey
E
.
Expression of the CB1 and CB2 receptor messenger RNAs during embryonic development in the rat
.
Neuroscience
.
1998 Feb
;
82
(
4
):
1131
49
. .
64.
Acevedo-Canabal
A
,
Colon-Cruz
L
,
Rodriguez-Morales
R
,
Varshney
GK
,
Burgess
S
,
Gonzalez-Sepulveda
L
,
Altered swimming behaviors in zebrafish larvae lacking cannabinoid receptor 2
.
Cannabis Cannabinoid Res
.
2019
;
4
(
2
):
88
101
.
65.
El-Brolosy
MA
,
Kontarakis
Z
,
Rossi
A
,
Kuenne
C
,
Gunther
S
,
Fukuda
N
,
Genetic compensation triggered by mutant mRNA degradation
.
Nature
.
2019 Apr
;
568
(
7751
):
193
7
.
66.
Etzion
T
,
Zmora
N
,
Zohar
Y
,
Levavi-Sivan
B
,
Golan
M
,
Gothilf
Y
.
Ectopic over expression of kiss1 may compensate for the loss of kiss2
.
Gen Comp Endocrinol
.
2020 Sep 1
;
295
:
113523
. .
67.
Watson
S
,
Chambers
D
,
Hobbs
C
,
Doherty
P
,
Graham
A
.
The endocannabinoid receptor, CB1, is required for normal axonal growth and fasciculation
.
Mol Cell Neurosci
.
2008 May
;
38
(
1
):
89
97
. .
68.
Berghuis
P
,
Dobszay
MB
,
Wang
X
,
Spano
S
,
Ledda
F
,
Sousa
KM
,
Endocannabinoids regulate interneuron migration and morphogenesis by transactivating the TrkB receptor
.
Proc Nat Aca Sci U S A
.
2005 Dec 27
;
102
(
52
):
19115
20
.
69.
Berghuis
P
,
Rajnicek
AM
,
Morozov
YM
,
Ross
RA
,
Mulder
J
,
Urban
GM
,
Hardwiring the brain: endocannabinoids shape neuronal connectivity
.
Science
.
2007 May 25
;
316
(
5828
):
1212
6
.
70.
Harkany
T
,
Guzmán
M
,
Galve-Roperh
I
,
Berghuis
P
,
Devi
LA
,
Mackie
K
.
The emerging functions of endocannabinoid signaling during CNS development
.
Trends Pharmacol Sci
.
2007 Feb
;
28
(
2
):
83
92
. .
71.
Sufian
MS
,
Amin
MR
,
Kanyo
R
,
Allison
WT
,
Ali
DW
.
CB1 and CB2 receptors play differential roles in early zebrafish locomotor development
.
J Experimen Bio
.
2019 Jun 28
.
72.
Kanyo
R
,
Amin
MR
,
Locskai
LF
,
Bouvier
DD
,
Olthuis
AM
,
Allison
WT
,
Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Delta(9)-tetrahydrocannabinol
.
Sci Rep
.
2021 Jun 1
;
11
(
1
):
11515
.
73.
Pan
YA
,
Choy
M
,
Prober
DA
,
Schier
AF
.
Robo2 determines subtype-specific axonal projections of trigeminal sensory neurons
.
Development
.
2012 Feb
;
139
(
3
):
591
600
. .
74.
Funakoshi
K
,
Nakano
M
,
Atobe
Y
,
Goris
RC
,
Kadota
T
,
Yazama
F
.
Differential development of TRPV1-expressing sensory nerves in peripheral organs
.
Cell Tissue Res
.
2006 Jan
;
323
(
1
):
27
41
. .
75.
Garami
A
,
Pakai
E
,
Oliveira
DL
,
Steiner
AA
,
Wanner
SP
,
Almeida
MC
,
Thermoregulatory phenotype of the Trpv1 knockout mouse: thermoeffector dysbalance with hyperkinesis
.
J Neurosci
.
2011 Feb 2
;
31
(
5
):
1721
33
.
76.
Corey
DP
,
Garcia-Anoveros
J
,
Holt
JR
,
Kwan
KY
,
Lin
SY
,
Vollrath
MA
,
TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells
.
Nature
.
2004 Dec 9
;
432
(
7018
):
723
30
.
77.
Johnstone
AD
,
de Leon
A
,
Unsain
N
,
Gibon
J
,
Barker
PA
.
Developmental axon degeneration requires TRPV1-dependent Ca(2+) Influx
.
eNeuro
.
2019 Jan–Feb
;
6
(
1
):
ENEURO.0019-19.2019
.
78.
Chechik
G
,
Meilijson
I
,
Ruppin
E
.
Neuronal regulation: a mechanism for synaptic pruning during brain maturation
.
Neural Comput
.
1999 Nov 15
;
11
(
8
):
2061
80
. .
79.
Crucke
J
,
Van de Kelft
A
,
Huysseune
A
.
The innervation of the zebrafish pharyngeal jaws and teeth
.
J Anat
.
2015 Jul
;
227
(
1
):
62
71
. .
80.
Vilceanu
D
,
Stucky
CL
.
TRPA1 mediates mechanical currents in the plasma membrane of mouse sensory neurons
.
PLoS One
.
2010 Aug 16
;
5
(
8
):
e12177
. .
81.
Cherif
H
,
Argaw
A
,
Cecyre
B
,
Bouchard
A
,
Gagnon
J
,
Javadi
P
,
Role of GPR55 during axon growth and target innervation
.
eNeuro
.
2015 Sep
;
2
(
5
):
ENEURO.0011-15.2015
.
82.
Guy
AT
,
Nagatsuka
Y
,
Ooashi
N
,
Inoue
M
,
Nakata
A
,
Greimel
P
,
Neuronal development. Glycerophospholipid regulation of modality-specific sensory axon guidance in the spinal cord
.
Science
.
2015 Aug 28
;
349
(
6251
):
974
7
.
83.
Ahmed
KT
,
Amin
MR
,
Shah
P
,
Ali
DW
.
Motor neuron development in zebrafish is altered by brief (5-h) exposures to THC ([9]-tetrahydrocannabinol) or CBD (cannabidiol) during gastrulation
.
Sci Rep
.
2018 Jul 12
;
8
(
1
):
10518
.
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