Introduction: Amnestic mild cognitive impairment (aMCI) represents an intermediate stage between normal aging and dementia. Hyperbaric oxygen (HBO) therapy has shown promise in enhancing brain oxygenation and promoting neural stem cell proliferation. Methods: Eighty SD male adult rats were randomly divided into control group, amnestic mild cognitive impairment group (aMCI group), and hyperbaric oxygen group (HBO group). The HBO group was divided into 6 subgroups according to different treatment pressures: 1.6 ATA subgroup, 1.8 ATA subgroup, 2.0 ATA subgroup, 2.2 ATA subgroup, 2.5 ATA subgroup, and 2.8 ATA subgroup, with 10 in each group. The HBO group received HBO therapy at the specified pressure for 60 min per day for 5 consecutive days. Results: After HBO treatment, compared with the aMCI group, the escape latency of each HBO subgroup was significantly shortened (p < 0.001). The 2.0 ATA subgroup (p = 0.001), 2.2 ATA subgroup (p = 0.001), and 2.5 ATA subgroup (p = 0.002) significantly increased the number of platform crossings. The levels of superoxide dismutase were significantly increased in 1.6 ATA subgroup (p = 0.019), 1.8 ATA subgroup (p = 0.003), 2.0 ATA subgroup (p = 0.010), and 2.2 ATA group (p = 0.016) and malondialdehyde contents were significantly decreased in the 1.6 ATA subgroup (p = 0.015), 1.8 ATA subgroup (p = 0.012), 2.0 ATA subgroup (p = 0.002), and 2.2 ATA subgroups (p < 0.001), and the levels of endothelial nitric oxide were significantly decreased in the 1.8 ATA subgroup (p = 0.007) and 2.0 ATA subgroup (p = 0.029), and the expression of neuronal nitric oxide were significantly decreased in the 1.8 ATA subgroup (p = 0.006), 2.0 ATA subgroup (p < 0.001), and the 2.2 ATA subgroup (p < 0.001). Conclusion: In aMCI model rats, HBO treatment at a pressure of 2.0 ATA with a stabilization time of 60 min per day for 5 days was the most effective.

1.
Bai
W
,
Chen
P
,
Cai
H
,
Zhang
Q
,
Su
Z
,
Cheung
T
, et al
.
Worldwide prevalence of mild cognitive impairment among community dwellers aged 50 years and older: a meta-analysis and systematic review of epidemiology studies
.
Age Ageing
.
2022
;
51
(
8
):
afac173
.
2.
Tifratene
K
,
Robert
P
,
Metelkina
A
,
Pradier
C
,
Dartigues
JF
.
Progression of mild cognitive impairment to dementia due to AD in clinical settings
.
Neurology
.
2015
;
85
(
4
):
331
8
.
3.
Feldmeier
J
.
Hyperbaric oxygen indications and results the hyperbaric oxygen therapy committee report
.
Kensington (MD)
:
Undersea and Hyperbaric Medical Society
;
2003
.
4.
Benedetti
S
,
Lamorgese
A
,
Piersantelli
M
,
Pagliarani
S
,
Benvenuti
F
,
Canestrari
F
.
Oxidative stress and antioxidant status in patients undergoing prolonged exposure to hyperbaric oxygen
.
Clin Biochem
.
2004
;
37
(
4
):
312
7
.
5.
Zhang
XY
,
Yang
YJ
,
Xu
PR
,
Zheng
XR
,
Wang
QH
,
Chen
CF
, et al
.
The role of beta-catenin signaling pathway on proliferation of rats neural stem cells after hyperbaric oxygen therapy in vitro
.
Cell Mol Neurobiol
.
2011
;
31
(
1
):
101
9
.
6.
Wang
Y
,
Mao
XO
,
Xie
L
,
Banwait
S
,
Marti
HH
,
Greenberg
DA
, et al
.
Vascular endothelial growth factor overexpression delays neurodegeneration and prolongs survival in amyotrophic lateral sclerosis mice
.
J Neurosci
.
2007
;
27
(
2
):
304
7
.
7.
Günther
A
,
Küppers-Tiedt
L
,
Schneider
PM
,
Kunert
I
,
Berrouschot
J
,
Schneider
D
, et al
.
Reduced infarct volume and differential effects on glial cell activation after hyperbaric oxygen treatment in rat permanent focal cerebral ischaemia
.
Eur J Neurosci
.
2005
;
21
(
11
):
3189
94
.
8.
Shwe
T
,
Bo-Htay
C
,
Ongnok
B
,
Chunchai
T
,
Jaiwongkam
T
,
Kerdphoo
S
, et al
.
Hyperbaric oxygen therapy restores cognitive function and hippocampal pathologies in both aging and aging-obese rats
.
Mech Ageing Dev
.
2021
;
195
:
111465
.
9.
Zhou
Z
,
Daugherty
WP
,
Sun
D
,
Levasseur
JE
,
Altememi
N
,
Hamm
RJ
, et al
.
Protection of mitochondrial function and improvement in cognitive recovery in rats treated with hyperbaric oxygen following lateral fluid-percussion injury
.
J Neurosurg
.
2007
;
106
(
4
):
687
94
.
10.
Chen
X
,
Li
Y
,
Chen
W
,
Nong
Z
,
Huang
J
,
Chen
C
.
Protective effect of hyperbaric oxygen on cognitive impairment induced by D-galactose in mice
.
Neurochem Res
.
2016
;
41
(
11
):
3032
41
.
11.
Zhang
X
,
Wang
X
,
Sun
X
,
Sun
X
,
Zhang
Y
,
Zhang
H
.
Differences in cognitive function of rats with traumatic brain injuries following hyperbaric oxygen therapy
.
Med Sci Monit
.
2016
;
22
:
2608
15
.
12.
Chen
C
,
Huang
L
,
Nong
Z
,
Li
Y
,
Chen
W
,
Huang
J
, et al
.
Hyperbaric oxygen prevents cognitive impairments in mice induced by D-galactose by improving cholinergic and anti-apoptotic functions
.
Neurochem Res
.
2017
;
42
(
4
):
1240
53
.
13.
Shapira
R
,
Solomon
B
,
Efrati
S
,
Frenkel
D
,
Ashery
U
.
Hyperbaric oxygen therapy ameliorates pathophysiology of 3xTg-AD mouse model by attenuating neuroinflammation
.
Neurobiol Aging
.
2018
;
62
:
105
19
.
14.
Körpınar
Ş
,
Uzun
H
.
The effects of hyperbaric oxygen at different pressures on oxidative stress and antioxidant status in rats
.
Medicina
.
2019
;
55
(
5
):
205
.
15.
Thomson
L
,
Paton
J
.
Oxygen toxicity
.
Paediatr Respir Rev
.
2014
;
15
(
2
):
120
3
.
16.
Oter
S
,
Korkmaz
A
,
Topal
T
,
Ozcan
O
,
Sadir
S
,
Ozler
M
, et al
.
Correlation between hyperbaric oxygen exposure pressures and oxidative parameters in rat lung, brain, and erythrocytes
.
Clin Biochem
.
2005
;
38
(
8
):
706
11
.
17.
Garcia
AJ
3rd
,
Dean
JB
,
Putnam
RW
.
Hyperbaric hyperoxia and normobaric reoxygenation increase excitability and activate oxygen-induced potentiation in CA1 hippocampal neurons
.
J Appl Physiol
.
2010
;
109
(
3
):
804
19
.
18.
Chen
Y
,
Nadi
NS
,
Chavko
M
,
Auker
CR
,
McCarron
RM
.
Microarray analysis of gene expression in rat cortical neurons exposed to hyperbaric air and oxygen
.
Neurochem Res
.
2009
;
34
(
6
):
1047
56
.
19.
Heyboer
M
3rd
,
Jennings
S
,
Grant
WD
,
Ojevwe
C
,
Byrne
J
,
Wojcik
SM
.
Seizure incidence by treatment pressure in patients undergoing hyperbaric oxygen therapy
.
Undersea Hyperb Med
.
2014
;
41
(
5
):
379
85
.
20.
Arieli
R.
.
Pulmonary oxygen toxicity in saturation dives with PO2 close to the lower end of the toxic range - A quantitative approach
.
Respir Physiol Neurobiol
.
2019
;
268
:
103243
.
21.
Demchenko
IT
,
Atochin
DN
,
Gutsaeva
DR
,
Godfrey
RR
,
Huang
PL
,
Piantadosi
CA
, et al
.
Contributions of nitric oxide synthase isoforms to pulmonary oxygen toxicity, local vs. mediated effects
.
Am J Physiol Lung Cell Mol Physiol
.
2008
;
294
(
5
):
L984
990
.
22.
Demchenko
IT
,
Welty-Wolf
KE
,
Allen
BW
,
Piantadosi
CA
.
Similar but not the same: normobaric and hyperbaric pulmonary oxygen toxicity, the role of nitric oxide
.
Am J Physiol Lung Cell Mol Physiol
.
2007
;
293
(
1
):
L229
38
.
23.
Klemetti
E
,
Rico-Vargas
S
,
Mojon
P
.
Short duration hyperbaric oxygen treatment effects blood flow in rats: pilot observations
.
Lab Anim
.
2005
;
39
(
1
):
116
21
.
24.
Chen
T
,
Zhou
Q
,
Xie
D
,
Ye
C
,
Huang
L
,
Lin
X
, et al
.
The method and effect of rapid establishment of a mild cognitive impairment model
.
Ann Palliat Med
.
2020
;
9
(
6
):
4119
26
.
25.
Zhao
B
,
Pan
Y
,
Wang
Z
,
Xu
H
,
Song
X
.
Hyperbaric oxygen pretreatment improves cognition and reduces hippocampal damage via p38 mitogen-activated protein kinase in a rat model
.
Yonsei Med J
.
2017
;
58
(
1
):
131
8
.
26.
Aguirre Siancas
EE
.
Influence of chewing behaviour on memory and spatial learning in albino BALB/c mice
.
Neurologia
.
2017
;
32
(
4
):
236
40
.
27.
Li
JJ
,
Zhu
Q
,
Lu
YP
,
Zhao
P
,
Feng
ZB
,
Qian
ZM
, et al
.
Ligustilide prevents cognitive impairment and attenuates neurotoxicity in D-galactose induced aging mice brain
.
Brain Res
.
2015
;
1595
:
19
28
.
28.
Hsieh
HM
,
Wu
WM
,
Hu
ML
.
Soy isoflavones attenuate oxidative stress and improve parameters related to aging and Alzheimer’s disease in C57BL/6J mice treated with D-galactose
.
Food Chem Toxicol
.
2009
;
47
(
3
):
625
32
.
29.
Farajdokht
F
,
Sadigh-Eteghad
S
,
Mahmoudi
J
.
Chapter 42: d-galactose-induced aging and brain mitochondria
. In:
Assessments, treatments and modeling in aging and neurological disease
;
2021
. p.
471
80
.
30.
Zhang
B
,
Lian
W
,
Zhao
J
,
Wang
Z
,
Liu
A
,
Du
G
.
DL0410 alleviates memory impairment in D-galactose-induced aging rats by suppressing neuroinflammation via the TLR4/MyD88/NF-κB pathway
.
Oxid Med Cell Longev
.
2021
;
2021
:
6521146
.
31.
Kumari
S
,
Dhapola
R
,
Sharma
P
,
Singh
SK
,
Reddy
DH
.
Implicative role of cytokines in neuroinflammation mediated AD and associated signaling pathways: current Progress in molecular signaling and therapeutics
.
Ageing Res Rev
.
2023
;
92
:
102098
.
32.
Cao
N
,
Liao
T
,
Liu
J
,
Fan
Z
,
Zeng
Q
,
Zhou
J
, et al
.
Clinical-grade human umbilical cord-derived mesenchymal stem cells reverse cognitive aging via improving synaptic plasticity and endogenous neurogenesis
.
Cell Death Dis
.
2017
;
8
(
8
):
e2996
.
33.
Matsunami
T
,
Sato
Y
,
Sato
T
,
Yukawa
M
.
Antioxidant status and lipid peroxidation in diabetic rats under hyperbaric oxygen exposure
.
Physiol Res
.
2010
;
59
(
1
):
97
104
.
34.
Knapp
LT
,
Klann
E
.
Potentiation of hippocampal synaptic transmission by superoxide requires the oxidative activation of protein kinase C
.
J Neurosci
.
2002
;
22
(
3
):
674
83
.
35.
Finley
J
.
Facilitation of hippocampal long-term potentiation and reactivation of latent HIV-1 via AMPK activation: common mechanism of action linking learning, memory, and the potential eradication of HIV-1
.
Med Hypotheses
.
2018
;
116
:
61
73
.
36.
Wang
X
,
Hu
X
,
Yang
Y
,
Takata
T
,
Sakurai
T
.
Systemic pyruvate administration markedly reduces neuronal death and cognitive impairment in a rat model of Alzheimer’s disease
.
Exp Neurol
.
2015
;
271
:
145
54
.
37.
Avshalumov
MV
,
Chen
BT
,
Rice
ME
.
Mechanisms underlying H(2)O(2)-mediated inhibition of synaptic transmission in rat hippocampal slices
.
Brain Res
.
2000
;
882
(
1–2
):
86
94
.
38.
Zhong
SZ
,
Ge
QH
,
Qu
R
,
Li
Q
,
Ma
SP
.
Paeonol attenuates neurotoxicity and ameliorates cognitive impairment induced by d-galactose in ICR mice
.
J Neurol Sci
.
2009
;
277
(
1–2
):
58
64
.
39.
Ren
X
,
Zou
L
,
Zhang
X
,
Branco
V
,
Wang
J
,
Carvalho
C
, et al
.
Redox signaling mediated by thioredoxin and glutathione systems in the central nervous system
.
Antioxid Redox Signal
.
2017
;
27
(
13
):
989
1010
.
40.
Fagan-Murphy
A
,
Hachoumi
L
,
Yeoman
MS
,
Patel
BA
.
Electrochemical sensor for the detection of multiple reactive oxygen and nitrogen species from ageing central nervous system homogenates
.
Mech Ageing Dev
.
2016
;
160
:
28
31
.
41.
Watson
JB
,
Arnold
MM
,
Ho
YS
,
O’Dell
TJ
.
Age-dependent modulation of hippocampal long-term potentiation by antioxidant enzymes
.
J Neurosci Res
.
2006
;
84
(
7
):
1564
74
.
42.
Tepić
S
,
Petković
A
,
Srejović
I
,
Jeremić
N
,
Zivković
V
,
Loncarević
S
, et al
.
Impact of hyperbaric oxygenation on oxidative stress in diabetic patients
.
Undersea Hyperb Med
.
2018
;
45
(
1
):
9
17
.
43.
Godman
CA
,
Joshi
R
,
Giardina
C
,
Perdrizet
G
,
Hightower
LE
.
Hyperbaric oxygen treatment induces antioxidant gene expression
.
Ann N Y Acad Sci
.
2010
;
1197
:
178
83
.
44.
Mihaljević
Z
,
Matić
A
,
Stupin
A
,
Rašić
L
,
Jukić
I
,
Drenjančević
I
.
Acute hyperbaric oxygenation, contrary to intermittent hyperbaric oxygenation, adversely affects vasorelaxation in healthy sprague-dawley rats due to increased oxidative stress
.
Oxid Med Cell Longev
.
2018
;
2018
:
7406027
.
45.
Bader
N
,
Bosy-Westphal
A
,
Koch
A
,
Rimbach
G
,
Weimann
A
,
Poulsen
HE
, et al
.
Effect of hyperbaric oxygen and vitamin C and E supplementation on biomarkers of oxidative stress in healthy men
.
Br J Nutr
.
2007
;
98
(
4
):
826
33
.
46.
Schottlender
N
,
Gottfried
I
,
Ashery
U
.
Hyperbaric oxygen treatment: effects on mitochondrial function and oxidative stress
.
Biomolecules
.
2021
;
11
(
12
):
1827
.
47.
Gubandru
M
,
Margina
D
,
Tsitsimpikou
C
,
Goutzourelas
N
,
Tsarouhas
K
,
Ilie
M
, et al
.
Alzheimer’s disease treated patients showed different patterns for oxidative stress and inflammation markers
.
Food Chem Toxicol
.
2013
;
61
:
209
14
.
48.
Demchenko
IT
,
Boso
AE
,
Whorton
AR
,
Piantadosi
CA
.
Nitric oxide production is enhanced in rat brain before oxygen-induced convulsions
.
Brain Res
.
2001
;
917
(
2
):
253
61
.
49.
Liu
W
,
Li
J
,
Sun
X
,
Liu
K
,
Zhang
JH
,
Xu
W
, et al
.
Repetitive hyperbaric oxygen exposures enhance sensitivity to convulsion by upregulation of eNOS and nNOS
.
Brain Res
.
2008
;
1201
:
128
34
.
50.
Cui
X
,
Zuo
P
,
Zhang
Q
,
Li
X
,
Hu
Y
,
Long
J
, et al
.
Chronic systemic D-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice: protective effects of R-alpha-lipoic acid
.
J Neurosci Res
.
2006
;
84
(
3
):
647
54
.
51.
Benari
O
,
Ravona‐Springer
R
,
Sano
M
,
Bendlin
BB
,
Livny
A
,
Almog
G
, et al
.
Hyperbaric oxygen therapy for older adults with T2D and mild cognitive impairment: a baseline characteristics
.
Alzheimer’s Dement
.
2024
;
20
(
S4
):
e092679
.
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