Introduction: Tempeh consumption has been linked to the improvement of cognitive function in older people. However, to what extent the amount of microorganism or the size of tempeh serving consumed per day influences the benefit to cognitive functions has not yet been studied. Methods: This experimental study involved a total of 90 respondents, who were divided into 3 groups: group A (consuming 100 g of Tempeh A/day), group B (consuming 100 g of Tempeh B/day), and group C (control). Intervention was given for 6 months. Cognitive assessments were done before and after the intervention. Blood uric acid level was checked at the end of intervention to examine the effect of tempeh consumption on this. The inclusion criteria were respondents aged 60 years or over with mild cognitive impairment (MCI) who agreed not to consume other fermented food during the study period. Respondents with diabetes were excluded. Results: There were 84 subjects at the end of the study, majority being female (71.4%) and aged over 65 years (72.6%). An increase in global cognitive scores was found in both groups A and B. The increase in language domain scores was found only in group A. Conclusion: Both Tempeh A or Tempeh B consumption for 6 months appeared to be beneficial in improving global cognitive function of older people with MCI. Consuming Tempeh A, which had a lower number of microorganisms, was also associated with an improvement in the language domain.

1.
Pessoa
RMP
,
Bomfim
AJL
,
Ferreira
BLC
,
Chagas
MHN
.
Diagnostic criteria and prevalence of mild cognitive impairment in older adults living in the community: a systematic review and meta-analysis
.
Arch. Clin. Psychiatry
.
2019
;
46
(
3
):
72
9
. .
2.
Alzheimers Diseases International
.
World Alzheimer report 2015 the global impact of dementia: an analysis of prevalence, incidence, cost and trends
.
2015
.
3.
Dominguez
LJ
,
Barbagallo
M
.
Nutritional prevention of cognitive decline and dementia
.
Acta Biomed
.
2018
;
89
(
2
):
276
90
. .
4.
Radisavljevic
N
,
Cirstea
M
,
Brett Finlay
B
.
Bottoms up: the role of gut microbiota in brain health
.
Environ Microbiol
.
2019
;
21
(
9
):
3197
211
. .
5.
Kowalski
K
,
Mulak
A
.
Brain-gut-microbiota axis in alzheimer’s disease
.
J Neurogastroenterol Motil
.
2019
;
25
(
1
):
48
60
. .
6.
Stephanie
S
,
Ratih
NK
,
Ratih
NK
,
Soka
S
,
Suwanto
A
.
Effect of tempeh supplementation on the profiles of human intestinal immune system and gut microbiota
.
Microbiol Indones
.
2017
;
11
(
1
):
11
7
. .
7.
Stephanie
S
,
Kartawidjajaputra
F
,
Silo
W
,
Yogiara
Y
,
Suwanto
A
.
Tempeh consumption enhanced beneficial bacteria in the human gut
.
Food Res
.
2019
;
3
:
57
63
. .
8.
Ahmad
A
,
Ramasamy
K
,
Jaafar
SM
,
Majeed
AB
,
Mani
V
.
Total isoflavones from soybean and tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain
.
Food Chem Toxicol
.
2014
;
65
:
120
8
. .
9.
Kridawati
A
,
Hardinsyah
H
,
Sulaeman
A
,
Rahardjo
TBW
,
Hogervorst
E
.
Tempe, Tofu, and Amyloid-β 1-40 serum levels in ovariectomized rats
.
J Alzheimers Dis
.
2020
;
76
(
1
):
159
63
. .
10.
Hogervorst
E
,
Mursjid
F
,
Priandini
D
,
Setyawan
H
,
Ismael
RI
,
Bandelow
S
, et al
Borobudur revisited: soy consumption may be associated with better recall in younger, but not in older, rural Indonesian elderly
.
Brain Res
.
2011
;
1379
:
206
12
. .
11.
Hamad
A
,
Mani
V
,
Ramasamy
K
,
Lim
SM
,
Majeed
ABA
.
Memory enhancement in rats by soybean and tempeh extracts is associated with improved cholinergic and reduced neuroinflammatory activities
.
Sains Malaysiana
.
2016
;
45
:
1299
310
.
12.
Auld
DS
,
Kornecook
TJ
,
Bastianetto
S
,
Quirion
R
.
Alzheimer’s disease and the basal forebrain cholinergic system: relations to beta-amyloid peptides, cognition, and treatment strategies
.
Prog Neurobiol
.
2002
;
68
(
3
):
209
45
. .
13.
Chan
Y-C
,
Lee
I-T
,
Wang
M-F
,
Yeh
W-C
,
Liang
B-C
.
Tempeh attenuates cognitive deficit, antioxidant imbalance, and amyloid β of senescence-accelerated mice by modulating Nrf2 expression via MAPK pathway
.
J Funct Foods
.
2018
;
50
:
112
9
. .
14.
Ayuningtyas
A
,
Murbawani
EA
,
Nuryanto
N
.
The effect of tempeh intake on spatial memory in prediabetic rats
.
Nutr Food Sci
.
2019
;
49
(
4
):
592
9
. .
15.
Tanuwidjaja
L
,
Hariyadi
HR
,
Mulyati
Y
.
Faktor-faktor yang mempengaruhi kualitas tempe di indonesia
.
JKTI
.
2000
;
1
:
31
5
.
16.
Welsh
KA
,
Butters
N
,
Mohs
RC
,
Beekly
D
,
Edland
S
,
Fillenbaum
G
, et al
The consortium to establish a registry for alzheimer’s disease (CERAD). Part V. A normative study of the neuropsychological battery
.
Neurology
.
1994
;
44
:
609
14
.
17.
Fjell
AM
,
Amlien
IK
,
Westlye
LT
,
Walhovd
KB
.
Mini-mental state examination is sensitive to brain atrophy in alzheimer’s disease
.
Dement Geriatr Cogn Disord
.
2009
;
28
(
3
):
252
8
. .
18.
Becker
JT
,
Mintun
MA
,
Diehl
DJ
,
Dobkin
J
,
Martidis
A
,
Madoff
DC
, et al
Functional neuroanatomy of verbal free recall: a replication study
.
Hum Brain Mapp
.
1994
;
1
(
4
):
284
92
. .
19.
Biesbroek
JM
,
van Zandvoort
MJ
,
Kuijf
HJ
,
Weaver
NA
,
Kappelle
LJ
,
Vos
PC
, et al
The anatomy of visuospatial construction revealed by lesion-symptom mapping
.
Neuropsychologia
.
2014
;
62
:
68
76
. .
20.
Baldo
JV
,
Arévalo
A
,
Patterson
JP
,
Dronkers
NF
.
Grey and white matter correlates of picture naming: evidence from a voxel-based lesion analysis of the boston naming test
.
Cortex
.
2013
;
49
(
3
):
658
67
. .
21.
Kim
B
,
Hong
VM
,
Yang
J
,
Hyun
H
,
Im
JJ
,
Hwang
J
, et al
A review of fermented foods with beneficial effects on brain and cognitive function
.
Prev Nutr Food Sci
.
2016
;
21
(
4
):
297
309
. .
22.
Zhou
L
.
Psychobiotics and the gut-brain axis: in the pursuit of happiness
.
Neuropsychiatr Dis Treat
.
2015
;
11
:
715
23
.
23.
Seumahu
CA
,
Suwanto
A
,
Rusmana
I
,
Solihin
DD
.
Bacterial and fungal communities in tempeh as reveal by amplified ribosomal intergenic sequence analysis
.
HAYATI J Biosci
.
2013
;
20
(
2
):
65
71
. .
24.
Hwang
YH
,
Park
S
,
Paik
JW
,
Chae
SW
,
Kim
DH
,
Jeong
DG
, et al
Efficacy and safety of lactobacillus plantarum C29-fermented soybean (DW2009) in individuals with mild cognitive impairment: a 12-week, multi-center, randomized, double-blind, placebo-controlled clinical trial
.
Nutrients
.
2019
;
11
(
1
):
305
.
25.
Dinan
TG
,
Stanton
C
,
Cryan
JF
.
Psychobiotics: a novel class of psychotropic
.
Biol Psychiatry
.
2013
;
74
(
10
):
720
6
. .
26.
Huang
SY
,
Chen
LH
,
Wang
MF
,
Hsu
CC
,
Chan
CH
,
Li
JX
, et al
Lactobacillus paracasei PS23 delays progression of age-related cognitive decline in senescence accelerated mouse prone 8 (SAMP8) mice
.
Nutrients
.
2018
;
10
(
7
):
1
13
. .
27.
Radita
R
,
Suwanto
A
,
Kurosawa
N
,
Wahyudi
AT
,
Rusmana
I
.
Firmicutes is the predominant bacteria in tempeh
.
Int Food Res J
.
2018
;
25
:
2313
20
.
28.
Barus
T
,
Suwanto
A
,
Tri Wahyudi
A
,
Wijaya
H
.
Role of bacteria in tempe bitter taste formation: microbiological and molecular biological analysis based on 16S rRNA gene
.
Microbiol Indones
.
2008
;
2
(
1
):
17
21
. .
29.
Sirilun
S
,
Sivamaruthi
BS
,
Kesika
P
,
Peerajan
S
,
Chaiyasut
C
.
Lactic acid bacteria mediated fermented soybean as a potent nutraceutical candidate
.
Asian Pac J Trop Biomed
.
2017
;
7
(
10
):
930
6
. .
30.
Lu
C
,
Wang
Y
,
Wang
D
,
Zhang
L
,
Lv
J
,
Jiang
N
, et al
Neuroprotective effects of soy isoflavones on scopolamine-induced amnesia in mice
.
Nutrients
.
2018
;
10
:
1
13
.
31.
Lalkovičová
M
,
Danielisová
VD
.
Neuroprotection and antioxidants
.
Neural Regen Res
.
2016 Jun
;
11
(
6
):
865
74
.
32.
Matsunaga
S
,
Fujishiro
H
,
Takechi
H
.
Efficacy and safety of cholinesterase inhibitors for mild cognitive impairment: a systematic review and meta-analysis
.
J Alzheimers Dis
.
2019
;
71
(
2
):
513
23
. .
33.
Tom C Russ
A
,
Morling
JR
.
Cholinesterase inhibitors for mild cognitive impairment
.
Cochrane Database Syst Rev
.
2012
;
2012
(
9
):
CD009132
.
34.
Messina
M
,
Messina
VL
,
Chan
P
.
Soyfoods, hyperuricemia and gout: a review of the epidemiologic and clinical data
.
Asia Pac J Clin Nutr
.
2011
;
20
(
3
):
347
58
.
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