Background: The function of a reconstructed bladder depends on the contracting bladder wall. This can be obtained with sufficient angiogenesis. Polyglycolic acid (PGA) is used as a cell vehicle for bladder reconstruction. Chitosan supports adhesion and differentiation of endothelial cells. The aim of the study was to compare PGA with PGA/chitosan‘sandwich’ grafts for bladder wall regeneration. Materials and Methods: 3T3 fibroblasts were seeded on 6 PGA and 3 chitosan scaffolds and incubated 3 days at 37ºC and 5% CO2 before implantation. Three rats underwent bladder reconstruction with PGA cell seeded grafts and 3 with PGA grafts covered with chitosan cell seeded grafts (‘sandwich’ graft). Three rats as controls were not operated on. After 6 months reconstructed tissue was stained with H&E. Vessels were identified by immunohistochemistry by CD31 and CD34 staining. A 4-level scale was used, (+++) referred to evident marker presence (high density of vessels) and (-) as lack of a marker (no vessels). Results: No complications during the 6-month period were noted. PGA/chitosan grafts were evaluated as (+) while PGA grafts were evaluated as (+) and (-) by the use of the CD31 antibody. The control group was evaluated as (+). PGA/chitosan grafts were evaluated as (++) and(+) while PGA grafts were evaluated as (++) and (-) by CD34 staining. The control group was evaluated as (+) and (-). Conclusion:Chitosan improved PGA abilities as a cell matrix and enhanced angiogenesis within the in vitro reconstructed graft.

This content is only available via PDF.
2008
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.