... remained a challenging issue. Here, we aimed to develop a protocol for electrotransfection of sheep EF cells. First, we optimized the pulsing condition using an OptiMEM-GlutaMAX medium as the electroporation buffer and found 2 pulses of 270 V, each for 10 ms and 10 s interval, is the most efficient...

...Anna Szabelska; Marcin R. Tatara; Witold Krupski This study was performed to determine morphological, densitometric, mechanical, and elemental characteristics of maxillary teeth in 5-month-old Polish Merino sheep. The total tooth volume (Tvol) was determined using quantitative computed tomography...

... of the presence of the solitary chemoreceptor cells (SCCs) in the VNO of Corriedale sheep were assessed using immunofluorescence. A protein gene product 9.5 (PGP 9.5), which is a neuronal marker recognized to be expressed in most neurons of vertebrate species, an olfactory marker protein (OMP) that is precise...

.... Besides the bone marrow, MSCs can be obtained from the dermis, which represents a large stem cell reservoir in the skin. Sheep provide an appropriate large animal model for preclinical studies. In this study, we focused on the isolation and characterization of MSCs from sheep dermis as an alternative...

... of this study was to evaluate the FTIRI changes in a large animal model of osteoporosis (female sheep with dietary induced metabolic acidosis; MA). Previous studies have investigated the relationship between bone material properties and bone strength in humans and smaller animals and have shown that changes...

...Lopeti T. Lavulo; Kitipong Uaesoontrachoon; Michiko Mirams; Jason D. White; Noelle E. Cockett; Eleanor J. Mackie; Charles N. Pagel Back and hind limb muscles of sheep paternally heterozygous for the callipyge single nucleotide polymorphism undergo extensive hypertrophy shortly after birth. We have...

... content is crucial for its metabolic and functional properties. We studied hyaluronan synthase (HAS) expression in knee joints of adult sheep as a novel cellular marker for chondrocyte function. Six house-bred Merino sheep (age 4–6 years) underwent two-stage surgery of their femoral condyles...

...]. In sheep, competition between littermates during pregnancy can reduce body weight by 100 days of gestation (unpubl. observations) which is associated with reduced muscle mass by 140 days of gestation [McCoard et al., 1997]. Muscle growth restriction during gestation is also associated with a reduction...

... Sheep The alginate bead culture system is an established technique for the culture of articular chondrocytes. This method has been shown to maintain correct phenotypic expression and cellular viability of chondrocytes for prolonged culture periods. Indeed, chondrocytes have been shown to regain...