...Beth Blake; Whitney Ann Ponwith; Klaus Rischka; Martin Wiesing; Tugba Ozdemir Introduction: Fibroblasts are central to a variety of homeostatic events such as wound healing and tissue regeneration. However, their pathologic activation is thought to play roles in a variety of diseases not only...

...-19). We cultured fibroblasts and nevus cells from the tissue specimens and then evaluated their inactivation after HHP at 200 MPa by confirming the attachment of the suspensions and by the live/dead staining of the suspensions, through the dissociation of the cells on chamber slides and by the live...

... efforts were undertaken to develop a TCSA that consisted of a dermal part and a differentiated multilayered epidermal part. Normal fibroblasts were expanded for 7 days and keratinocytes for 9 days in monolayer cultures. After this initial expansion phase the isolated cells were seeded into the scaffold...

...Wendy M.W. Tra; Johan W. van Neck; Steven E.R. Hovius; Gerjo J.V.M. van Osch; Soledad Perez-Amodio The aim of this study was to create and characterize a tissue-engineered mucosal equivalent (TEM) that closely resembles native mucosa. TEM consists of human primary keratinocytes and fibroblasts...

... of such mineral-inducing conditions on differentiation of human periodontal ligament (PDL) fibroblasts into osteoblasts/cementoblasts was analyzed in three-dimensional collagen gels. Mineral-inducing conditions decreased collagen type I gene expression and induced dentin matrix protein 1 (DMP1; a marker of late...

...Shuichi Mizuno; Julie Glowacki Endochondral bone formation is induced by demineralized bone powder (DBP) when DBP is implanted subcutaneously in rodents. Previously, we developed an in vitro model of this process, wherein human dermal fibroblasts (hDFs) differentiate to chondrocytes when cultured...

... study two well-characterised model systems, involving dermal fibroblasts seeded onto silicone membranes and chondrocytes seeded in agarose constructs, have been used to study the effects of mechanical loading on cellular activity. The data presented demonstrate that both the tensile conditioning...

... on collagen-coated surfaces and when defects were filled with collagen gels, but there was no cell growth on surfaces with poly- L -lysine or laminin coating. Immunocytochemistry identified the migrating cells mainly as fibroblasts with some intermingled epithelial cells. Scanning electron microscopy proved...