... and TIMP2 were small influenced by siTGF-β2, the extracellular matrix and transcription factor expressions showed to be significantly reduced in double knockdown palate compared to single knockdown palates. Conclusions: This study demonstrates that double siRNAs targeting TGF-β2 and -β3 results...

...Cecibel M. León-Félix; Andrea Q. Maranhão; Christiani A. Amorim; Carolina M. Lucci Introduction: The decellularized extracellular matrix (dECM) from ovarian tissue could be the best scaffold for the development of a transplantable artificial ovary. Typically, dECM from ovarian tissue has been...

... the cell type of interest for a specific application. One particularly interesting and powerful signaling cue is the extracellular matrix (ECM) surrounding stem cells, a network of biopolymers that, along with cells, makes up what we define as a tissue. The composition, structure, biochemical features...

...Rebecca Louisthelmy; Brycen M. Burke; R. Chase Cornelison Cell-derived matrices are useful tools for studying the extracellular matrix (ECM) of different cell types and testing the effects on cell migration or wound repair. These matrices typically are generated using extended culture with ascorbic...

..., and lipids. A novel extracellular vesicle, the matrix-bound nanovesicle (MBV), has been recently discovered. The MBV is similar to the microvesicle, however, it is attached to the extracellular matrix, instead of being secreted. This review compares MBVs to other types of extracellular vesicles to try...

... by showing that it was feasible to rebuild an organ using a decellularized extracellular matrix scaffold. Toward the goal of clinical translation of bioengineered tissues and organs, there is increasing recognition of the underlying need to better integrate basic science domains and industry. From...

...Thai Thanh Hoang Thi; Diem Huong Tran Nguyen; Dinh Tien Dung Nguyen; Dai Hai Nguyen; Minh-Dung Truong The aim of this study was to develop a porcine epiphyseal plate-derived extracellular matrix powder (PEPEP) for epiphyseal plate regeneration. PEPEP was characterized by chemical assay to determine...

... hydrolysis, can modulate diverse cellular actions via adenosine receptors. The objectives of this study were to investigate the effects of exogenous adenosine on the production of extracellular matrix (ECM; i.e., collagen type II and aggrecan) and ATP of IVD cells and explore the underlying mechanism...

... is increasing with the advancement of technologies and new treatments in human and animal regenerative medicine. Thus, decellularized placental extracellular matrix (ECM) has emerged as a new tool for the production of biological scaffolds for subsequent recellularization and implantation for recovery...

...Marko Jović; Ivan Nikolić; Vera Todorović; Aleksandar Petrović; Vladimir Petrović; Marijola Mojsilović; Tijana Denčić In the absence of systematized data on the extracellular matrix components during prenatal liver development, the present study aimed to investigate the time of appearance...

... concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. In the current review, we...

... are expressed as the mean ± SEM from at least 3 independent experiments. Paired t tests were performed using GraphPad Prism. Vascularized cardiac spheroids Cardiac fibrosis 3D in vitro model Transforming growth factor beta 1 Doxorubicin Extracellular matrix Apoptosis Cardiomyocytes...

...Gili Kaufman; Laiz Nunes; Alex Eftimiades; Wojtek Tutak Tissue engineering-based therapies rely on the delivery of monolayered fibroblasts on two-dimensional polystyrene-coated and extracellular matrix (ECM) surfaces to regenerate connective tissues. However, this approach may fail to mimic...

... concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. In the current review, we...

...Amit Aurora; Benjamin T. Corona; Thomas J. Walters Volumetric muscle loss (VML) results in irrecoverable loss of muscle tissue making its repair challenging. VML repair with acellular extracellular matrix (ECM) scaffolds devoid of exogenous cells has shown improved muscle function, but limited de...

... bone marrow-derived mesenchymal stem cell (BM-MSC) behaviors. We generated tissue microenvironments using conditioned medium (CM) and extracellular matrix (ECM) samples obtained from human heart and umbilical cord tissue explant cultures and by tissue decellularization. Mass spectrometry...

...Zhao Huang; Benjamin Kohl; Maria Kokozidou; Stephan Arens; Gundula Schulze-Tanzil Tissue-engineered intervertebral discs (IVDs) utilizing decellularized extracellular matrix (ECM) could be an option for the reconstruction of impaired IVDs due to degeneration or injury. The objective of this study...

..., instructions or products referred to in the content or advertisements. Automatic decellularisation Enzymatic digestion Extracellular matrix Porcine aorta As the number of people affected by organ failure and cardiovascular disease continues to grow around the world, tissue engineering...

... birth, they are a high-yield source for the isolation of stem/progenitor cells and are rich in extracellular matrix (ECM), representing an important resource for regenerative medicine purposes. Interactions among cells, ECM and bioactive molecules regulate tissue and organ generation and comprise...

... Scaffold Endothelial cell Extracellular matrix Artery Decellularization Tissue engineering The tissue-native extracellular matrix (ECM) represents the best possible environment for a cell because of its biochemical signaling and its structure, promoting cell adhesion, proliferation...