... by ameloblast cells in the tooth germ, prior to eruption of the tooth into the oral cavity. Secreted enamel proteins direct mineralization patterns during the maturation stage of amelogenesis as the tooth prepares to erupt. The amelogenins are the most abundant enamel proteins and are required for normal enamel...

...) in either a three-dimensional culture system, or in the renal capsules of SCID mice. When co-cultured with FDMCs in vitro, ES-ECs polarized and expressed amelogenin. Tooth organ-like structures assembled with epithelium and encased mesenchyme and developing enamel-like structures could be detected...

...K. Tanimoto; Y.C. Huang; Y. Tanne; R. Kunimatsu; M. Michida; M. Yoshioka; N. Ozaki; T. Sasamoto; Y. Yoshimi; Y. Kato; K. Tanne Amelogenins are the major constituent of developing extracellular enamel matrix proteins and are understood to have an exclusively epithelial origin. Recent studies have...

...Yulei Huang; Michel Goldberg; Thuan Le; Ran Qiang; Douglas Warner; Halina Ewa Witkowska; Haichuan Liu; Li Zhu; Pamela DenBesten; Wu Li Amelogenins containing exons 8 and 9 are alternatively spliced variants of amelogenin. Some amelogenin spliced variants have been found to promote pulp regeneration...

...Ping-An Fang; Henry C. Margolis; James F. Conway; James P. Simmer; Gary H. Dickinson; Elia Beniash Cryogenic transmission electron microscopy (cryo-EM) was used to explore the self-assembly of recombinant murine amelogenin (rM179) in vitro.Our cryo-EM data showed that amelogenin self-assembly...

...E. Le Norcy; S.-Y. Kwak; F.B. Wiedemann-Bidlack; E. Beniash; Y. Yamakoshi; J.P. Simmer; H.C. Margolis N-terminal and C-terminal (CT) domains of amelogenin have been shown to be essential for proper enamel formation. Recent studies have also suggested that although the C-terminus plays an apparent...

...Keith M. Bromley; Rajamani Lakshminarayanan; Ya-Ping Lei; Malcolm L. Snead; Janet Moradian-Oldak Two point mutations (T21I and P40T) within amelogenin have been identified from human DNA sequences in 2 instances of amelogenesis imperfecta. We studied the folding and self-assembly of recombinant...

... expression, distribution of amelogenin, enamelin and DSP proteins, cell proliferation localization and dedifferentiation of secretory ameloblasts. Importantly, our data indicate that specific levels of Amelx and Dspp gene expression define whether mouse CMV induces enamel agenesis or hypoplasia. We used...

...Li Zhu; Kotaro Tanimoto; Thuan Le; Pamela K. DenBesten; Wu Li Amelogenins, the chief proteins in enamel matrix, undergo progressive degradation by matrix metalloproteinase-20 (MMP-20) to facilitate crystal growth. Proline is the most abundant residue in amelogenin and is located upstream to all MMP...

...Carolyn W. Gibson; Yong Li; Bill Daly; Cynthia Suggs; Zhi-an Yuan; Hanson Fong; Darrin Simmons; Melissa Aragon; Ashok B. Kulkarni; J. Timothy Wright Introduction: The amelogenin proteins secreted by ameloblasts during dental enamel development are required for normal enamel structure. Amelx null...

... of amelogenins on apatite crystallites in developing porcine enamel. Cross-sections of developing enamel tissue from freeze-fractured pig third molar were treated with antiserum against recombinant mouse amelogenin and immunoreactivity confirmed by Western blot analysis. The samples were then treated...

...Mineyo Iwase; Satoko Kaneko; Hielim Kim; Yoko Satta; Naoyuki Takahata Amelogenin (AMEL) arose prior to the emergence of tetrapods and transposed into an intron of the Rho GTPase-activating protein 6 gene. In the mammalian lineage leading to eutherians, a pair of homologous autosomes...

... to in the content or advertisements. Amelogenesis imperfecta Amelogenin Enamel Enamelin Enamelysin Kallikrein 4 Cells Tissues Organs 2007;186:78 85 DOI: 10.1159/000102683 Enamel Formation and Amelogenesis Imperfecta Jan C.-C. Hu Yong-Hee P. Chun Turki Al Hazzazzi James P. Simmer University of Michigan...

... proteins – amelogenin (AMELX), enamelin (ENAM), and ameloblastin (AMBN) – and a matrix metalloproteinase, enamelysin (MMP20). Enamel formation proceeds with the steady elongation of the enamel crystals at a mineralization front just beneath the ameloblast distal membrane, where these proteins are secreted...

...Carolyn W. Gibson; Ashok B. Kulkarni; J. Timothy Wright Amelogenin proteins are secreted by ameloblast cells during tooth development. Because of extensive alternative splicing of the amelogenin primary RNA transcript, and because systematic proteolysis results in many additional peptides during...

...Janet Moradian-Oldak; Michel Goldberg Tooth enamel is formed in the extracellular space within an organic matrix enriched in amelogenin proteins. Amelogenin nanosphere assembly is a key factor in controlling the oriented and organized growth of enamel apatite crystals. Recently, we have reported...

... aspects of preameloblast phenotypes. In addition, the expression of amelogenin, another component of the enamel matrix, was specifically reduced in mutant ameloblasts. More than 20% of amelobastin-null mice developed odontogenic tumors. We also found that recombinant ameloblastin specifically bound...

... kD. Using fluorescent-tagged tuftelin and amelogenin plasmid constructs we saw little evidence that these two enamel proteins colocalize in ameloblast-like LS-8 cells. Tuftelin is primarily localized to distinct ‘speckled’ domains within the cell cytoplasm. In an attempt to better define...

...Michael L. Paine; Dan-Hong Zhu; Wen Luo; Malcolm L. Snead The secreted, full-length amelogenin is the dominant protein of the forming enamel organ. As enamel mineralization progresses, amelogenin is quickly subjected to proteolytic activity, and eliminated from the enamel environment. Mature enamel...