Abstract
Millions of people suffer from damage or disease to the nervous system that results in a loss of myelin, such as through a spinal cord injury or multiple sclerosis. Diminished myelin levels lead to further cell death in which unmyelinated neurons die. In the central nervous system, a loss of myelin is especially detrimental because of its poor ability to regenerate. Cell therapies such as stem or precursor cell injection have been investigated as stem cells are able to grow and differentiate into the damaged cells; however, stem cell injection alone has been unsuccessful in many areas of neural regeneration. Therefore, researchers have begun exploring combined therapies with biomaterials that promote cell growth and differentiation while localizing cells in the injured area. The regrowth of myelinating oligodendrocytes from neural stem cells through a biomaterials approach may prove to be a beneficial strategy following the onset of demyelination. This article reviews recent advancements in biomaterial strategies for the differentiation of neural stem cells into oligodendrocytes, and presents new data indicating appropriate properties for oligodendrocyte precursor cell growth. In some cases, an increase in oligodendrocyte differentiation alongside neurons is further highlighted for functional improvements where the biomaterial was then tested for increased myelination both in vitro and in vivo.
References
1.
Aboody, K., A. Capela, N. Niazi, J.H. Stern, S. Temple (2011) Translating stem cell studies to the clinic for CNS repair: current state of the art and the need for a Rosetta stone. Neuron 70: 597-613.
2.
Aguado, B.A., W. Mulyasasmita, J. Su, K.J. Lampe, S.C. Heilshorn (2012) Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. Tissue Eng Part A 18: 806-815.
3.
Aizawa, Y., N.D. Leipzig, T. Zahira, M.S. Shoichet (2008) The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels. Biomaterials 29: 4676-4683.
4.
Andersen, S.L. (2003) Trajectories of brain development: point of vulnerability or window of opportunity? Neurosci Biobehav Rev 27: 3-18.
5.
Asmani, M.N., J. Ai, G. Amoabediny, A. Noroozi, M. Azami, S. Ebrahimi-Barough, M. Navaei-Nigjeh, A. Ai, M. Jafarabadi (2013) Three-dimensional culture of differentiated endometrial stromal cells to oligodendrocyte progenitor cells (OPCs) in fibrin hydrogel. Cell Biol Int 37: 1340-1349.
6.
Aurand, E.R., K.J. Lampe, K.B. Bjugstad (2012) Defining and designing polymers and hydrogels for neural tissue engineering. Neurosci Res 72: 199-213.
7.
Bakshi, A., C.A. Keck, V.S. Koshkin, D.G. LeBold, R. Siman, E.Y. Snyder, T.K. McIntosh (2005) Caspase-mediated cell death predominates following engraftment of neural progenitor cells into traumatically injured rat brain. Brain Res 1065: 8-19.
8.
Bible, E., D.Y. Chau, M.R. Alexander, J. Price, K.M. Shakesheff, M. Modo (2009) The support of neural stem cells transplanted into stroke-induced brain cavities by PLGA particles. Biomaterials 30: 2985-2994.
9.
Blurton-Jones, M., M. Kitazawa, H. Martinez-Coria, N.A. Castello, F.J. Muller, J.F. Loring, T.R. Yamasaki, W.W. Poon, K.N. Green, F.M. LaFerla (2009) Neural stem cells improve cognition via BDNF in a transgenic model of Alzheimer disease. Proc Natl Acad Sci USA 106: 13594-13599.
10.
Bradbury, E.J., L.D.F. Moon, R.J. Popat, V.R. King, G.S. Bennet, P.N. Patel, J.W. Fawcett, S.B. McMahon (2002) Chondroitinase ABC promotes functional recovery after spinal cord injury. Nature 416: 636-640.
11.
Bradl, M., H. Lassmann (2010) Oligodendrocytes: biology and pathology. Acta Neuropathol 119: 37-53.
12.
Burdick, J.A., M. Ward, E. Liang, M.J. Young, R. Langer (2006) Stimulation of neurite outgrowth by neurotrophins delivered from degradable hydrogels. Biomaterials 27: 452-459.
13.
Burns, A.J., N. Thapar (2014) Neural stem cell therapies for enteric nervous system disorders. Nat Rev Gastroenterol Hepatol 11: 317-328.
14.
Cao, Q., R.L. Benton, S.R. Whittemore (2002) Stem cell repair of central nervous system injury. J Neurosci Res 68: 501-510.
15.
Carenini, S., D. Montag, M. Schachner, R. Martini (1998) MAG-deficient Schwann cells myelinate dorsal root ganglion neurons in culture. Glia 22: 213-220.
16.
Chew, S.Y., R. Mi, A. Hoke, K.W. Leong (2008) The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation. Biomaterials 29: 653-661.
17.
Cholas, R.H., H.P. Hsu, M. Spector (2012) The reparative response to cross-linked collagen-based scaffolds in a rat spinal cord gap model. Biomaterials 33: 2050-2059.
18.
Cooke, M.J., K. Vulic, M.S. Shoichet (2010) Design of biomaterials to enhance stem cell survival when transplanted into the damaged central nervous system. Soft Matter 6: 4988.
19.
Diao, H.J., W.C. Low, U. Milbreta, Q.R. Lu, S.Y. Chew (2015) Nanofiber-mediated microRNA delivery to enhance differentiation and maturation of oligodendroglial precursor cells. J Control Release 208: 85-92.
20.
Elias, P.Z., M. Spector (2012) Implantation of a collagen scaffold seeded with adult rat hippocampal progenitors in a rat model of penetrating brain injury. J Neurosci Methods 209: 199-211.
21.
Fawcett, J.W., R.A. Asher (1999) The glial scar and central nervous system repair. Brain Res Bull 49: 337-391.
22.
Gelain, F., A. Lomander, A.L. Vescovi, S. Zhang (2007) Systematic studies of a self-assembling peptide nanofiber scaffold with other scaffolds. J Nanosci Nanotechnol 7: 424-434.
23.
Gingras, M., M.M. Beaulieu, V. Gagnon, H.D. Durham, F. Berthod (2008) In vitro study of axonal migration and myelination of motor neurons in a three-dimensional tissue-engineered model. Glia 56: 354-364.
24.
Goddaard, D.R., M. Berry, A.M. Butt (1999) In vivo actions of fibroblast growth factor-2 and insulin-like growth factor-I on oligodendrocyte development and myelination in the central nervous system. J Neurosci Res 57: 74-85.
25.
Gunn, J.W., S.D. Turner, B.K. Mann (2005) Adhesive and mechanical properties of hydrogels influence neurite extension. J Biomed Mater Res A 72: 91-97.
26.
Hammang, J.P., D.R. Archer, I.D. Duncan (1997) Myelination following transplantation of EGF-responsive neural stem cells into a myelin-deficient environment. Exp Neurol 147: 84-95.
27.
Horne, M.K., D.R. Nisbet, J.S. Forsythe, C.L. Parish (2010) Three-dimensional nanofibrous scaffolds incorporating immobilized BDNF promote proliferation and differentiation of cortical neural stem cells. Stem Cells Dev 19: 843-852.
28.
Hsieh, A., T. Zahir, Y. Lapitsky, B. Amsden, W. Wan, M.S. Shoichet (2010) Hydrogel/electrospun fiber composites influence neural stem/progenitor cell fate. Soft Matter 6: 2227.
29.
Jeong, S.W., K. Chu, K.H. Jung, S.U. Kim, M. Kim, J.K. Roh (2003) Human neural stem cell transplantation promotes functional recovery in rats with experimental intracerebral hemorrhage. Stroke 34: 2258-2263.
30.
Johnson, P.J., A. Tatara, D.A. McCreedy, A. Shiu, S.E. Sakiyama-Elbert (2010) Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI. Soft Matter 6: 5127-5137.
31.
Kandel, E.R., J.H. Schwartz, T.M. Jessell (2000) Principles of Neural Science, ed 4. New York, McGraw-Hill.
32.
Karandikar, N.J., M.P. Crawford, X. Yan, R.B. Ratts, J.M. Brenchley, D.R. Ambrozak, A.E. Lovett-Racke, E.M. Frohman, P. Stastny, D.C. Douek, R.A. Koup, M.K. Racke (2002) Glatiramer acetate (Copaxone) therapy induces CD8+ T cell responses in patients with multiple sclerosis. J Clin Invest 109: 641-649.
33.
Ke, Y., L. Chi, R. Xu, C. Luo, D. Gozal, R. Liu (2006) Early response of endogenous adult neural progenitor cells to acute spinal cord injury in mice. Stem Cells 24: 1011-1019.
34.
Kirby, B.B., N. Takada, A.J. Latimer, J. Shin, T.J. Carney, R.N. Kelsh, B. Appel (2006) In vivo time-lapse imaging shows dynamic oligodendrocyte progenitor behavior during zebrafish development. Nat Neurosci 9: 1506-1511.
35.
Kuhlmann, T., V. Miron, Q. Cui, C. Wegner, J. Antel, W. Bruck (2008) Differentiation block of oligodendroglial progenitor cells as a cause for remyelination failure in chronic multiple sclerosis. Brain 131: 1749-1758.
36.
Lampe, K.J., A.L. Antaris, S.C. Heilshorn (2013) Design of three-dimensional engineered protein hydrogels for tailored control of neurite growth. Acta Biomater 9: 5590-5599.
37.
Lampe, K.J., K.B. Bjugstad, M.J. Mahoney (2010a) Impact of degradable macromer content in a PEG hydrogel on neural cell metabolic activity, redox state, proliferation, and differentiation. Tissue Eng Part A 16: 1857-1866.
38.
Lampe, K.J., S.C. Heilshorn (2012) Building stem cell niches from the molecule up through engineered peptide materials. Neurosci Lett 519: 138-146.
39.
Lampe, K.J., R.G. Mooney, K.B. Bjugstad, M.J. Mahoney (2010b) Effect of macromer weight percent on neural cell growth in 2D and 3D nondegradable PEG hydrogel culture. J Biomed Mater Res A 94: 1162-1171.
40.
Lampe, K.J., R. Namba, T. Silverman, K. Bjugstad, M. Mahoney (2009) Impact of lactic acid on cell proliferation and free radical-induced cell death in monolayer cultures of neural precursor cells. Biotechnol Bioeng 103: 1214-1223.
41.
Lee, S., S.Y.C. Chong, S.J. Tuck, J.M. Corey, J.R. Chan (2013) A rapid and reproducible assay for modeling myelination by oligodendrocytes using engineered nanofibers. Nat Protoc 8: 771-782.
42.
Lee, S., M.K. Leach, S.A. Redmond, S.Y. Chong, S.H. Mellon, S.J. Tuck, Z.Q. Feng, J.M. Corey, J.R. Chan (2012) A culture system to study oligodendrocyte myelination processes using engineered nanofibers. Nat Methods 9: 917-922.
43.
Leipzig, N.D., M.S. Shoichet (2009) The effect of substrate stiffness on adult neural stem cell behavior. Biomaterials 30: 6867-6878.
44.
Leipzig, N.D., R.G. Wylie, H. Kim, M.S. Shoichet (2011) Differentiation of neural stem cells in three-dimensional growth factor-immobilized chitosan hydrogel scaffolds. Biomaterials 32: 57-64.
45.
Li, H., A.M. Koenig, P. Sloan, N.D. Leipzig (2014) In vivo assessment of guided neural stem cell differentiation in growth factor immobilized chitosan-based hydrogel scaffolds. Biomaterials 35: 9049-9057.
46.
Li, H., A. Wijekoon, N.D. Leipzig (2012) 3D differentiation of neural stem cells in macroporous photopolymerizable hydrogel scaffolds. PLoS One 7: e48824.
47.
Li, X., X. Liu, L. Cui, C. Brunson, W. Zhao, N.R. Bhat, N. Zhang, X. Wen (2013) Engineering an in situ crosslinkable hydrogel for enhanced remyelination. FASEB J 27: 1127-1136.
48.
Liu, C., J.C. Sage, M.R. Miller, R.G. Verhaak, S. Hippenmeyer, H. Vogel, O. Foreman, R.T. Bronson, A. Nishiyama, L. Luo, H. Zong (2011) Mosaic analysis with double markers reveals tumor cell of origin in glioma. Cell 146: 209-221.
49.
Lodish, H., A. Berk, S.L. Zipursky, P. Matsudaira, D. Balitimore, J. Darnell (2000) Molecular Cell Biology. New York, Freeman.
50.
Martens, P.J., S.J. Bryant, K.S. Anseth (2003) Tailoring the degradation of hydrogels formed from multivinyl poly(ethylene glycol) and poly(vinyl alcohol) macromers for cartilage tissue engineering. Biomacromolecules 4: 283-292.
51.
Martin, B.C., E.J. Minner, S.L. Wiseman, R.L. Klank, R.J. Gilbert (2008) Agarose and methylcellulose hydrogel blends for nerve regeneration applications. J Neural Eng 5: 221-231.
52.
McDonald, J.W., M.J. Howard (2002) Repairing the damaged spinal cord: a summary of our early success with embryonic stem cell transplantation and remyelination. Prog Brain Res 137: 299-309.
53.
Mekhail, M., G. Almazan, M. Tabrizian (2012) Oligodendrocyte-protection and remyelination post-spinal cord injuries: a review. Prog Neurobiol 96: 322-339.
54.
Morell, P., R.H. Quarles (1999) Basic Neurochemistry: Molecular, Cellular and Medical Aspects. Philadelphia, Lippincott-Raven.
55.
Murphy, W.L., T.C. McDevitt, A.J. Engler (2014) Materials as stem cell regulators. Nat Mater 13: 547-557.
56.
Najm, F.J., A.M. Lager, A. Zaremba, K. Wyatt, A.V. Caprariello, D.C. Factor, R.T. Karl, T. Maeda, R.H. Miller, P.J. Tesar (2013) Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol 31: 426-433.
57.
Nakamura, M., H. Okano (2013) Cell transplantation therapies for spinal cord injury focusing on induced pluripotent stem cells. Cell Res 23: 70-80.
58.
Nisbet, D.R., K.E. Crompton, M.K. Horne, D.I. Finkelstein, J.S. Forsythe (2008) Neural tissue engineering of the CNS using hydrogels. J Biomed Mater Res Part B Appl Biomater 87: 251-263.
59.
Noble, M., M. Mayer-Proschel, C. Proschel (2013) iOPs: a new tool for studying myelin pathologies? Cell Stem Cell 12: 503-504.
60.
Paintlia, A.S., M.K. Paintlia, A.K. Singh, I. Singh (2008) Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis. Mol Pharmacol 73: 1381-1393.
61.
Pathan, S.A., Z. Iqbal, S.M.A. Zaidi, S. Talegaonkar, D. Vohra, G.K. Jain, A. Azeem, N. Jain, J.R. Lalani, R.K. Khar, F.J. Ahmad (2009) CNS drug delivery systems: novel approaches. Recent Pat Drug Deliv Formul 3: 71-89.
62.
Pfeiffer, S.E., A.E. Warrington, R. Bansal (1993) The oligodendrocyte and its many cellular processes. Trends Cell Biol 3: 191-197.
63.
Pittier, R., F. Sauthier, J.A. Hubbell, H. Hall (2005) Neurite extension and in vitro myelination within three-dimensional modified fibrin matrices. J Neurobiol 63: 1-14.
64.
Pohl, H.B., C. Porcheri, T. Mueggler, L.C. Bachmann, G. Martino, D. Riethmacher, R.J. Franklin, M. Rudin, U. Suter (2011) Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage. J Neurosci 31: 1069-1080.
65.
Rafalski, V.A., P.P. Ho, J.O. Brett, D. Ucar, J.C. Dugas, E.A. Pollina, L.M. Chow, A. Ibrahim, S.J. Baker, B.A. Barres, L. Steinman, A. Brunet (2013) Expansion of oligodendrocyte progenitor cells following SIRT1 inactivation in the adult brain. Nat Cell Biol 15: 614-624.
66.
Riess, P., C. Zhang, K.E. Saatman, H.L. Laurer, L.G. Longhi, R. Raghupathi, P.M. Lenzlinger, J. Lifshitz, J. Boockvar, E. Neugebauer, E.Y. Snyder, T.K. McIntosh (2002) Transplanted neural stem cells survive, differentiate, and improve neurological motor function after experimental traumatic brain injury. Neurosurgery 51: 1043-1052.
67.
Sack, I., K.J. Streitberger, D. Krefting, F. Paul, J. Braun (2011) The influence of physiological aging and atrophy on brain viscoelastic properties in humans. PLoS One 6: e23451.
68.
Saha, K., A.J. Keung, E.F. Irwin, Y. Li, L. Little, D.V. Schaffer, K.E. Healy (2008) Substrate modulus directs neural stem cell behavior. Biophys J 95: 4426-4438.
69.
Sakiyama-Elbert, S.E., J.A. Hubbell (2001) Functional biomaterials: design of novel biomaterials. Annu Rev Mater Res 31: 183-201.
70.
Schmidt, C.E., J.B. Leach (2003) Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng 5: 293-347.
71.
Schnell, E., K. Klinkhammer, S. Balzer, G. Brook, D. Klee, P. Dalton, J. Mey (2007) Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend. Biomaterials 28: 3012-3025.
72.
Schouten, J.W., C.T. Fulp, N.C. Royo, K.E. Saatman, D.J. Watson, E.Y. Snyder, J.Q. Trojanowski, D.J. Prockop, A.I.R. Maas, T.K. McIntosh (2004) A review and rationale for the use of cellular transplantation as a therapeutic strategy for traumatic brain injury. J Neurotrauma 21: 1501-1538.
73.
Shreiber, D.I., H. Hao, R.A. Elias (2009) Probing the influence of myelin and glia on the tensile properties of the spinal cord. Biomech Model Mechanobiol 8: 311-321.
74.
Silva, G.A., C. Czeisler, K.L. Niece, E. Beniash, D.A. Harrington, J.A. Kessler, S.I. Stupp (2004) Selective differentiation of neural progenitor cells by high-epitope density nanofibers. Science 303: 1352-1355.
75.
Silva, N.A., A.J. Salgado, R.A. Sousa, J.T. Joliveira, A.J. Pedro, H. Leite-Almeida, R. Cerqueira, A. Almeida, F. Mastronardi, J.F. Mano, N.M. Neves, N. Sousa, R.L. Reis (2010) Development and characterization of a novel hybrid tissue engineering-based scaffold for spinal cord injury repair. Tissue Eng Part A 16: 45-54.
76.
Sindou, M.P., P. Mertens, L. Garcia-Larrea (2001) Surgical procedures for neuropathic pain. Neurosurg Quart 11: 45-65.
77.
Soderquist, R.G., E.M. Sloane, L.C. Loram, J.A. Harrison, E.C. Dengler, S.M. Johnson, L.D. Amer, C.S. Young, M.T. Lewis, S. Poole, M.G. Frank, L.R. Watkins, E.D. Milligan, M.J. Mahoney (2010) Release of plasmid DNA-encoding IL-10 from PLGA microparticles facilitates long-term reversal of neuropathic pain following a single intrathecal administration. Pharm Res 27: 841-854.
78.
Stabenfeldt, S.E., H.R. Irons, M.C. LaPlaca (2011) Stem cells and bioactive scaffolds as a treatment for tramatic brain injury. Curr Stem Cell Res Ther 6: 208-220.
79.
Stabenfeldt, S.E., M.C. LaPlaca (2011) Variations in rigidity and ligand density influence neuronal response in methylcellulose-laminin hydrogels. Acta Biomater 7: 4102-4108.
80.
Stabenfeldt, S.E., G. Munglani, A.J. Garcia, M.C. LaPlaca (2010) Biomimetic microenvironment modulates neural stem cell survival, migration, and differentiation. Tissue Eng Part A 16: 3747-3758.
81.
Subramanian, A., U.M. Krishnan, S. Sethuraman (2009) Development of biomaterial scaffold for nerve tissue engineering: biomaterial mediated neural regeneration. J Biomed Sci 16: 108.
82.
Syed, Y.A., A.S. Baer, G. Lubec, H. Hoeger, G. Widhalm, M.R. Kotter (2008) Inhibition of oligodendrocyte precursor cell differentiation by myelin-associated proteins. Neurosurg Focus 24: E5.
83.
Tam, R.Y., M.J. Cooke, M.S. Shoichet (2012) A covalently modified hydrogel blend of hyaluronan-methyl cellulose with peptides and growth factors influences neural stem/progenitor cell fate. J Mater Chem 22: 19402.
84.
Taylor, Z., K. Miller (2004) Reassessment of brain elasticity for analysis of biomechanisms of hydrocephalus. J Biomech 37: 1263-1269.
85.
Thomas, A.M., S.K. Seidlits, A.G. Goodman, T.V. Kukushliev, D.M. Hassani, B.J. Cummings, A.J. Anderson, L.D. Shea (2014) Sonic hedgehog and neurotrophin-3 increase oligodendrocyte numbers and myelination after spinal cord injury. Integr Biol 6: 694-705.
86.
Trapp, B.D., K.A. Nave (2008) Multiple sclerosis: an immune or neurodegenerative disorder? Annu Rev Neurosci 31: 247-269.
87.
van Lith, R., E.K. Gregory, J. Yang, M.R. Kibbe, G.A. Ameer (2014) Engineering biodegradable polyester elastomers with antioxidant properties to attenuate oxidative stress in tissues. Biomaterials 35: 8113-8122.
88.
Wang, H.B., M.E. Mullins, J.M. Cregg, A. Hurtado, M. Oudega, M.T. Trombley, R.J. Gilbert (2009) Creation of highly aligned electrospun poly-L-lactic acid fibers for nerve regeneration applications. J Neural Eng 6: 016001.
89.
Wilkinson, A.E., L.J. Kobelt, N.D. Leipzig (2014) Immobilized ECM molecules and the effects of concentration and surface type on the control of NSC differentiation. J Biomed Mater Res A 102: 3419-3428.
90.
Willerth, S.M., T.E. Faxel, D.I. Gottlieb, S.E. Sakiyama-Elbert (2007) The effects of soluble growth factors on embryonic stem cell differentiation inside of fibrin scaffolds. Stem Cells 25: 2235-2244.
91.
Willerth, S.M., A. Rader, S.E. Sakiyama-Elbert (2008) The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds. Stem Cell Res 1: 205-218.
92.
Woerly, S., V.D. Doan, N. Sosa, J. de Vellis, A. Espinosa-Jeffrey (2004) Prevention of gliotic scar formation by NeuroGel allows partial endogenous repair of transected cat spinal cord. J Neurosci Res 75: 262-272.
93.
Woerly, S., P. Petrov, E. Sykova, T. Roitbak, Z. Simonova, A.R. Harvey (1999) Neural tissue formation within porous hydrogels implanted in brain and spinal cord lesions: ultrastructural, immunohistochemical, and diffusion studies. Tissue Eng 5: 467-488.
94.
Woerly, S., E. Pinet, L. De Robertis, M. Bousmina, G. Laroche, T. Roitback, L. Vargova, E. Sykova (1998) Heterogeneous PHPMA hydrogels for tissue repair and axonal regeneration in the injured spinal cord. J Biomater Sci Polym Ed 9: 681-711.
95.
Xiong, Y., J.X. Zhu, Z.Y. Fang, C.G. Zeng, C. Zhang, G.L. Qi, M.H. Li, W. Zhang, D.P. Quan, J. Wan (2012) Coseeded Schwann cells myelinate neurites from differentiated neural stem cells in neurotrophin-3-loaded PLGA carriers. Int J Nanomed 7: 1977-1989.
96.
Yang, N., J.B. Zuchero, H. Ahlenius, S. Marro, Y.H. Ng, T. Vierbuchen, J.S. Hawkins, R. Geissler, B.A. Barres, M. Wernig (2013) Generation of oligodendroglial cells by direct lineage conversion. Nat Biotechnol 31: 434-439.
97.
Ylä-Outinen, L., T. Joki, M. Varjola, H. Skottman, S. Narkilahti (2014) Three-dimensional growth matrix for human embryonic stem cell-derived neuronal cells. J Tissue Eng Regen Med 8: 186-194.
98.
Yu, D., G.A. Silva (2008) Stem cell sources and therapeutic approaches for central nervous system and neural retinal disorders. Neurosurg Focus 24: E11.
99.
Zhang, M., D. Methot, V. Poppa, Y. Fujio, K. Walsh, C.E. Murry (2001) Cardiomyocyte grafting for cardiac repair: graft cell death and anti-death strategies. J Mol Cell Cardiol 33: 907-921.
100.
Zhong, Y., R.V. Bellamkonda (2008) Biomaterials for the central nervous system. J R Soc Interface 5: 957-975.
101.
Zuchero, J.B., B.A. Barres (2015) Glia in mammalian development and disease. Development 142: 3805-3809.
102.
Zustiak, S.P., S. Pubill, A. Ribeiro, J.B. Leach (2013) Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds as a cell delivery vehicle: characterization of PC12 cell response. Biotechnol Prog 29: 1255-1264.
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