Adipose tissue precursor cells (pre-adipocytes) are part of a stromal vascular fraction that can be easily isolated from fat tissue. Adipose tissue can be harvested by 2 methods: aspiration and excision. We analyzed whether the pre-adipocyte yield, growth characteristics and ability to differentiate into mature adipose tissue are influenced by the type of harvesting procedure. Adipose tissue was simultaneously harvested from the abdomen by surgical excision or aspiration according to the Coleman procedure in 10 individuals. This permitted inter- and intra-individual comparisons. Cell viability and yield were determined directly after isolation of pre-adipocytes. The growth kinetics were investigated in culture. Furthermore, pre-adipocytes were cultured under adipogenic conditions to compare their differentiation potential. The number of viable pre-adipocytes was significantly higher after excision of adipose tissue compared to aspiration. The proliferation kinetic was not influenced by the type of harvesting. No differences were observed in the differentiation potential of the pre-adipocytes between both groups. Compared to excision, aspiration of adipose tissue negatively affects the yield of pre-adipocytes. However, growth characteristics and differentiation potential of viable cultured cells are not influenced by the type of surgical harvesting. Due to its reduced donor site morbidity, we conclude that aspiration of adipose tissue is a valid harvesting method for isolation of pre-adipocytes.

1.
Allen, R.J., P. Treece (1994) Deep inferior epigastric perforator flap for breast reconstruction. Ann Plast Surg 32: 32–38.
2.
Bjorntorp, P., M. Karlsson, P. Pettersson, G. Sypniewska (1980) Differentiation and function of rat adipocyte precursor cells in primary culture. J Lipid Res 21: 714–723.
3.
Coleman, S.R. (2006) Structural fat grafting: more than a permanent filler. Plast Reconstr Surg 118: 108S–120S.
4.
Eppley, B.L., R.A. Sidner, J.M. Platis, A.M. Sadove (1992) Bioactivation of free-fat transfers: a potential new approach to improving graft survival. Plast Reconstr Surg 90: 1022–1030.
5.
Ersek, R.A. (1991) Transplantation of purified autologous fat: a 3-year follow-up is disappointing. Plast Reconstr Surg 87: 219–227; discussion 228.
6.
Futter, C.M., M.H. Webster, S. Hagen, S.L. Mitchell (2000) A retrospective comparison of abdominal muscle strength following breast reconstruction with a free TRAM or DIEP flap. Br J Plast Surg 53: 578–583.
7.
Gorjup, E., L. Peter, S. Wien, H. von Briesen, D. Schmitt (2009) Automated microscopic quantification of adipogenic differentiation of human gland stem cells. Ann Anat 191: 13–22.
8.
Hamdi, M., K. Van Landuyt, J.B. Hijjawi, N. Roche, P. Blondeel, S. Monstrey (2008) Surgical technique in pedicled thoracodorsal artery perforator flaps: a clinical experience with 99 patients. Plast Reconstr Surg 121: 1632–1641.
9.
Har-Shai, Y., E. Lindenbaum, O. Ben-Itzhak, B. Hirshowitz (1996) Large liponecrotic pseudocyst formation following cheek augmentation by fat injection. Aesthetic Plast Surg 20: 417–419.
10.
Hemmrich, K., D. von Heimburg, R. Rendchen, C. Di Bartolo, E. Milella, N. Pallua (2005) Implantation of preadipocyte-loaded hyaluronic acid-based scaffolds into nude mice to evaluate potential for soft tissue engineering. Biomaterials 26: 7025–7037.
11.
Huss, F.R., G. Kratz (2002) Adipose tissue processed for lipoinjection shows increased cellular survival in vitro when tissue engineering principles are applied. Scand J Plast Reconstr Surg Hand Surg 36: 166–171.
12.
Lalikos, J.F., Y.Q. Li, T.P. Roth, J.W. Doyle, W.E. Matory, W.T. Lawrence (1997) Biochemical assessment of cellular damage after adipocyte harvest. J Surg Res 70: 95–100.
13.
Mandrekas, A.D., G.J. Zambacos, C. Kittas (1998) Cyst formation after fat injection. Plast Reconstr Surg 102: 1708–1709.
14.
Nguyen, A., K.A. Pasyk, T.N. Bouvier, C.A. Hassett, L.C. Argenta (1990) Comparative study of survival of autologous adipose tissue taken and transplanted by different techniques. Plast Reconstr Surg 85: 378–386; discussion 387–389.
15.
Oedayrajsingh-Varma, M.J., S.M. van Ham, M. Knippenberg, M.N. Helder, J. Klein-Nulend, T.E. Schouten, M.J. Ritt, F.J. van Milligen (2006) Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy 8: 166–177.
16.
Patrick, C.W. Jr., P.B. Chauvin, J. Hobley, G.P. Reece (1999) Preadipocyte seeded PLGA scaffolds for adipose tissue engineering. Tissue Eng 5: 139–151.
17.
Peer, L.A. (1956) The neglected free fat graft, its behavior and clinical use. Am J Surg 92: 40–47.
18.
Ramirez-Zacarias, J.L., F. Castro-Munozledo, W. Kuri-Harcuch (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97: 493–497.
19.
Schoeller, T., S. Lille, G. Wechselberger, A. Otto, A. Mowlavi, H. Piza-Katzer (2001) Histomorphologic and volumetric analysis of implanted autologous preadipocyte cultures suspended in fibrin glue: a potential new source for tissue augmentation. Aesthetic Plast Surg 25: 57–63.
20.
Scudiero, D.A., R.H. Shoemaker, K.D. Paull, A. Monks, S. Tierney, T.H. Nofziger, M.J. Currens, D. Seniff, M.R. Boyd (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48: 4827–4833.
21.
Sommer, B., G. Sattler (2000) Current concepts of fat graft survival: histology of aspirated adipose tissue and review of the literature. Dermatol Surg 26: 1159–1166.
22.
Torio-Padron, N., N. Baerlecken, A. Momeni, G.B. Stark, J. Borges (2007) Engineering of adipose tissue by injection of human preadipocytes in fibrin. Aesthetic Plast Surg 31: 285–293.
23.
Ullmann, Y., M. Hyams, Y. Ramon, D. Beach, I.J. Peled, E.S. Lindenbaum (1998) Enhancing the survival of aspirated human fat injected into nude mice. Plast Reconstr Surg 101: 1940–1944.
24.
van Harmelen, V., T. Skurk, K. Rohrig, Y.M. Lee, M. Halbleib, I. Aprath-Husmann, H. Hauner (2003) Effect of BMI and age on adipose tissue cellularity and differentiation capacity in women. Int J Obes Relat Metab Disord 27: 889–895.
25.
von Heimburg, D., K. Hemmrich, S. Haydarlioglu, H. Staiger, N. Pallua (2004) Comparison of viable cell yield from excised versus aspirated adipose tissue. Cells Tissues Organs 178: 87–92.
26.
von Heimburg, D., S. Zachariah, A. Low, N. Pallua (2001) Influence of different biodegradable carriers on the in vivo behavior of human adipose precursor cells. Plast Reconstr Surg 108: 411–420; discussion 421–422.
27.
Wechselberger, G., R.C. Russell, M.W. Neumeister, T. Schoeller, H. Piza-Katzer, C. Rainer (2002) Successful transplantation of three tissue-engineered cell types using capsule induction technique and fibrin glue as a delivery vehicle. Plast Reconstr Surg 110: 123–129.
28.
Wu, L.C., A. Bajaj, D.W. Chang, P.M. Chevray (2008) Comparison of donor-site morbidity of SIEA, DIEP, and muscle-sparing TRAM flaps for breast reconstruction. Plast Reconstr Surg 122: 702–709.
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