The scapholunate interosseous ligament (SLIL) is biomechanically important in maintaining wrist motion and grip strength in the hand, but its possible sensory role in the dynamic muscular stability of the wrist joint has not been examined. The aim of this study was to use immunohistochemical methods to analyze the general innervation and the possible existence of sensory corpuscles in the SLIL. The ligament was excised in its entirety from 9 patients. Antibodies against the low-affinity p75 neurotrophic receptor (p75) were used to reveal sensory corpuscles as well as general innervation. Furthermore, antibodies against the general nerve marker protein gene product 9.5 (PGP 9.5) and the glial marker S-100 were used to additionally depict innervation and corpuscular structures. Blood vessels occurred in areas interspersed throughout the homogenous collagenous structure. In these vascularized areas, the SLIL was found to be supplied with nerve fascicles and sensory corpuscles of both the Ruffini and lamellated type. p75 immunoreactivity (IR) was detected in association with the nerve fascicles and the corpuscles, particularly in their capsule. S-100 IR was found in the Schwann cells in the central regions of the corpuscle, and PGP 9.5 IR marked the axonal structures in the corpuscles. New information on neurotrophin receptor distribution in ligaments has been obtained here. The presence of nerve fascicles and particularly sensory corpuscles in the SLIL suggests that the ligament has a proprioceptive role in the stability of the wrist. The marked p75 IR further indicates that neurotrophins play a part in a proprioceptive system in the ligament, given the importance of neurotrophins in maintaining sensory function.

Keywords:
Scapholunate, Mechanoreceptors, Innervation, ligament, p75, Ligament, scapholunate interosseous
1.
Albuerne, M., S. Lopez, F.J. Naves, A. Martinez-Almagro, J. Represa, J.A. Vega (1998) S100alpha and S100beta proteins in human cutaneous sensory corpuscles: Effects of nerve and spinal cord injury. Anat Rec 251: 351–359.
2.
Ashton, I.K., B.A. Ashton, S.J. Gibson, J.M. Polak, D.C. Jaffray, S.M. Eisenstein (1992) Morphological basis for back pain: The demonstration of nerve fibers and neuropeptides in the lumbar facet joint capsule but not in ligamentum flavum. J Orthop Res 10: 72–78.
3.
Barrack, R.L., H.B. Skinner (1990) The sensory function of knee ligaments; in Daniel, D.M., W.H. Akeson, J.J. O’Connor (eds): Knee Ligaments: Structure, Function, Injury and Repair. New York, Raven, pp 95–114.
4.
Berger, R.A. (2001) The anatomy of the ligaments of the wrist and distal radioulnar joints. Clin Orthop 383: 32–40.
5.
Berger, R.A., T. Imeada, L. Berglund, K.N. An (1999) Constraint and material properties of the subregions of the scapholunate interosseous ligament. J Hand Surg [Am] 24: 953–962.
6.
Berger, R.A., J.M. Kauer, J.M. Landsmeer (1991) Radioscapholunate ligament: A gross anatomic and histologic study of fetal and adult wrists. J Hand Surg [Am] 16: 350–355.
7.
Byers, M.R. (1990) Segregation of NGF receptor in sensory receptors, nerves and local cells of teeth and periodontium demonstrated by EM immunocytochemistry. J Neurocytol 19: 765–775.
8.
Curtis, R., J.R. Tonra, J.L. Stark, K.M. Adryan, J.S. Park, K.D. Cliffer, R.M. Lindsay, P.S. DiStefano (1998) Neuronal injury increases retrograde axonal transport of the neurotrophins to spinal sensory neurons and motor neurons via multiple receptor mechanisms. Mol Cell Neurosci 12: 105–118.
9.
Del Valle, M.E., S.F. Harwin, A. Maestro, A. Murcia, J.A. Vega (1998) Immunohistochemical analysis of mechanoreceptors in the human posterior cruciate ligament: A demonstration of its proprioceptive role and clinical relevance. J Arthroplasty 13: 916–922.
10.
Denti, M., M. Monteleone, A. Berardi, A.S. Panni (1994) Anterior cruciate ligament mechanoreceptors. Histologic studies on lesions and reconstruction. Clin Orthop 308: 29–32.
11.
Ernfors, P. (2001) Local and target-derived actions of neurotrophins during peripheral nervous system development. Cell Mol Life Sci 58:1036–1044.
12.
Fundin, B.T., I. Silos-Santiago, P. Ernfors, A.M. Fagan, H. Aldskogius, T.M. DeChiara, H.S. Phillips, M. Barbacid, G.D. Yancopoulos, F.L. Rice (1997) Differential dependency of cutaneous mechanoreceptors on neurotrophins, trk receptors, and P75 LNGFR. Dev Biol 190: 94–116.
13.
Garcia-Elias, M. (1999) Carpal instabilites and dislocations; in Green, D.P., R.N. Hotchkiss, W.C. Pederson (eds): Green’s Operative Hand Surgery. Philadelphia, Churchill Livingstone, vol 1, pp 865–928.
14.
Gonzalez-Martinez, T., P. Perez-Pinera, B. Diaz-Esnal, J.A. Vega (2003) S-100 proteins in the human peripheral nervous system. Microsc Res Tech 60: 633–638.
15.
Halata, Z., C. Wagner, K.I. Baumann (1999) Sensory nerve endings in the anterior cruciate ligament (Lig. cruciatum anterius) of sheep. Anat Rec 254: 13–21.
16.
Hansson, M., S. Forsgren (1995) Immunoreactive atrial and brain natriuretic peptides are co-localized in Purkinje fibres but not in the innervation of the bovine heart conduction system. Histochem J 27: 222–230.
17.
Ibanez, C.F. (1998) Emerging themes in structural biology of neurotrophic factors. Trends Neurosci 21: 438–444.
18.
Johansson, H., P. Sjolander, P. Sojka (1991a) Receptors in the knee joint ligaments and their role in the biomechanics of the joint. Crit Rev Biomed Eng 18: 341–368.
19.
Johansson, H., P. Sjolander, P. Sojka (1991b) A sensory role for the cruciate ligaments. Clin Orthop (268): 161–178.
20.
Konttinen, Y.T., S. Santavirta, P. Paavolainen, I. Antti-Poika, T. Tiainen, D. Nordstrom, M. Hukkanen (1992) Immunoreactive neuropeptide nerves in ligamentous tissue in chronic shoulder pain. Arch Orthop Trauma Surg 111: 341–344.
21.
Kopp, D.M., J.T. Trachtenberg, W.J. Thompson (1997) Glial growth factor rescues Schwann cells of mechanoreceptors from denervation-induced apoptosis. J Neurosci 17: 6697–6706.
22.
Koshima, I., T. Moriguchi (1999) Denervation of Pacinian corpuscles: Electron microscopic observations in the rat following nerve transection. J Reconstr Microsurg 15: 273–279.
23.
Lieber, R.L., B.O. Ljung, J. Friden (1997) Intraoperative sarcomere length measurements reveal differential design of human wrist extensor muscles. J Exp Biol 200: 19–25.
24.
Linscheid, R.L., J.H. Dobyns (1992) Treatment of scapholunate dissociation. Rotatory subluxation of the scaphoid. Hand Clin 8: 645–652.
25.
Linscheid, R.L., J.H. Dobyns (2002) Dynamic carpal stability. Keio J Med 51: 140–147.
26.
Lopez, S.M., M. Perez-Perez, J.M. Marquez, F.J. Naves, J. Represa, J.A. Vega (1998) p75 and TrkA neurotrophin receptors in human skin after spinal cord and peripheral nerve injury, with special reference to sensory corpuscles. Anat Rec 251: 371–383.
27.
Michelson, J.D., C. Hutchins (1995) Mechanoreceptors in human ankle ligaments. J Bone Joint Surg Br 77: 219–224.
28.
Morisawa, Y. (1998) Morphological study of mechanoreceptors on the coracoacromial ligament. J Orthop Sci 3: 102–110.
29.
O’Meeghan, C.J., W. Stuart, V. Mamo, J.K. Stanley, I.A. Trail (2003) The natural history of an untreated isolated scapholunate interosseus ligament injury. J Hand Surg [Br] 28: 307–310.
30.
Ribeiro-da-Silva, A., R.L. Kenigsberg, A.C. Cuello (1991) Light and electron microscopic distribution of nerve growth factor receptor-like immunoreactivity in the skin of the rat lower lip. Neuroscience 43: 631–646.
31.
Short, W.H., F.W. Werner, J.K. Green, S. Masaoka (2002) Biomechanical evaluation of ligamentous stabilizers of the scaphoid and lunate. J Hand Surg [Am] 27: 991–1002.
32.
Sjolander, P., H. Johansson, M. Djupsjobacka (2002) Spinal and supraspinal effects of activity in ligament afferents. J Electromyogr Kinesiol 12: 167–176.
33.
Solomonow, M., M. Krogsgaard (2001) Sensorimotor control of knee stability. A review. Scand J Med Sci Sports 11: 64–80.
34.
Stark, B., M. Risling, T. Carlstedt (2001) Distribution of the neurotrophin receptors p75 and trkB in peripheral mechanoreceptors; observations on changes after injury. Exp Brain Res 136: 101–107.
35.
Strasmann, T., Z. Halata, S.K. Loo (1987) Topography and ultrastructure of sensory nerve endings in the joint capsules of the Kowari (Dasyuroides byrnei), an Australian marsupial. Anat Embryol (Berl) 176: 1–12.
36.
Tang, J.B., J. Ryu, S. Omokawa, S. Wearden (2002) Wrist kinetics after scapholunate dissociation: The effect of scapholunate interosseous ligament injury and persistent scapholunate gaps. J Orthop Res 20: 215–221.
37.
Ulfhake, B., E. Bergman, B.T. Fundin (2002) Impairment of peripheral sensory innervation in senescence. Auton Neurosci 96: 43–49.
38.
Vandenabeele, F., J. Creemers, I. Lambrichts, W. Robberechts (1995) Fine structure of vesiculated nerve profiles in the human lumbar facet joint. J Anat 187: 681–692.
39.
Vangsness, C.T., Jr., M. Ennis, J.G. Taylor, R. Atkinson (1995) Neural anatomy of the glenohumeral ligaments, labrum, and subacromial bursa. Arthroscopy 11: 180–184.
40.
Vega, J.A., M.E. Del Valle, J.J. Haro, B. Calzada, S. Suarez-Garnacho, L. Malinovsky (1993) Nerve growth factor receptor immunoreactivity in Meissner and Pacinian corpuscles of the human digital skin. Anat Rec 236: 730–736.
41.
Zelena, J. (1984) The effect of long-term denervation on the ultrastructure of Pacinian corpuscles in the cat. Cell Tissue Res 238: 387–394.
42.
Zimny, M.L. (1988) Mechanoreceptors in articular tissues. Am J Anat 182: 16–32.
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2004
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