Saphenous vein (SV) and internal mammary artery (IMA) are used for aorto-coronary bypass grafting. IMA is considered to be the graft of choice for coronary revascularization having a long-term patency compared to SV. The aim of this study is to investigate the structure of vascular wall using a new technical approach. We analysed the chemical composition of vessel wall layers (total lipid, lipid ester and protein) of 25 vascular segments (19 SV and 6 IMA) using Fourier transform infrared spectroscopy (FTIR). FTIR analysis showed that in intima layer lipid ester and protein concentration (expressed as arbitrary units) was significantly higher in SV (lipid ester = 0.020 ± 0.002; protein = 0.449 ± 0.022) than in IMA (lipid ester = 0.014 ± 0.002; protein = 0.342 ± 0.032). Moreover, the percentage of lipid ester on total lipid was significantly higher in SV (intima = 54.7 ± 2.9%; media = 78.4 ± 4.9%; adventitia = 83.9 ± 8.3%) wall layers compared to IMA ones (intima = 37.3 ± 4.9%; media = 45.4 ± 3.8; adventitia = 57.1 ± 4.8). These data suggest that a different chemical composition of wall layers could also be responsible for the morphological modifications observed in SV after grafting.

Genter, J.M., E. Wentrup-Byrne, P.J. Walker, M.D. Walsh (1998) Comparison of fresh and post-mortem human arterial tissue: An analysis using FT-IR microspectroscopy and chemometrics. Cell Mol Biol 44: 251–259.
Goldman, S., K. Zadina, T. Moritz, T. Ovitt, G. Sethi, J. Copeland, W. Henderson (2001) Prospective ten-year patency of saphenous vein and left internal mammary artery grafts after coronary artery bypass surgery J Am Coll Cardiol 37: 1A–64A.
Hsu, H.H.T., N.C. Camacho, O. Tawfik, F. Sun (2002) Induction of calcification in rabbit aortas by high cholesterol diets: Roles of calcifiable vesicles in dystrophic calcification. Atherosclerosis 161: 85–94.
Kodaly, D.R., D.M. Small, J. Powell, K. Krishnan (1991) Infrared micro-imaging of atherosclerotic arteries. Appl Spectrosc 45: 1310–1317.
Manoharan, R., J.J. Baraga, R.P. Rava, R.R. Dasari, M. Fitzmaurice, M.S. Feld (1993) Biochemical analysis and mapping of atherosclerotic human artery using FT-IR microspectroscopy. Atherosclerosis 103: 181–193.
Mantovani, D., P. Vermette, M. Fiset, R. Guidoin, G. Laroche (2000) Modeling lipid uptake in expanded polyfluoroethylene vascular prosthesis and its effects on mechanical properties. Artif Organs 24: 334–345.
Mantovani, D., P. Vermette, R. Guidoin, G. Laroche (1999) Lipid uptake in synthetic vascular prostheses explanted from humans. Biomaterials 20: 1023–1032.
Melin, A.M., A. Perromat, G. Deleris (2001) Sensitivity of Deinococcus radiodurans to gamma-irradiation: A novel approach by Fourier transform infrared spectroscopy. Arch Biochem Biophys 394: 265–274.
Mulvihill, N.T., J. Marco (2002) Percutaneous intervention for atherosclerotic disease in saphenous vein grafts. Int J Cardiol 83: 103–110.
Paschalis, E.P., F. Betts, E. DiCarlo, R. Mendelsohn, A.L. Boskey (1997) FTIR microspectroscopic analysis of normal human cortical and trabecular bone. Calcif Tissue Int 61: 480–486.
Petruzzo P., A. Cappai, G. Brotzu, B. Batetta, M. Putzolu, M.F. Mulas, R.R. Bonatesta, F. Sanna, S. Dessi (2001) Lipid metabolism and molecular changes in normal and atherosclerotic vessels. Eur J Vasc Endovasc Surg 22: 31–36.
Schultz, C.P., K.Z. Liu, P.D. Kerr, H.H. Mantsch (1998) In situ infrared histopathology of keratinization in human oral/oropharyngeal squamous cell carcinoma. Oncol Res 10: 277–286.
Shi Y., S. Patel, K.L. Davenpeck, R. Niculescu, E. Rodriguez, M.G. Magno, M.L. Ormont, J.D. Mannion, A. Zalewski (2001) Oxidative stress and lipid retention in vascular grafts: Comparison between venous and arterial conduits. Circulation 103: 2408–2413.
Stamou, S.C., S.W. Boyce, H.C. Cooke, B.D. Carlos, L.C. Sweet, P.J. Corso (2002) One-year outcome after combined coronary artery bypass grafting and transmyocardial laser revascularization for refractory angina pectoris. Am J Cardiol 89: 1365–1368.
Thiene, G., P. Miazzi, M. Valsecchi, M. Valente, U. Bortolotti (1980) Histological survey of the saphenous vein before its use as autologous aortocoronary by pass graft. Thorax 35: X519–522.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.