The present study was designed to examine the distribution of interglobular dentine in human tooth roots. The material comprised 17 teeth, of which 3 were premolars extracted for orthodontic reasons from children 10–12 years of age and the other teeth (4 incisors, 3 canines and 7 molars) were extracted for periodontitis from individuals aged 32–63 years. All teeth were free of caries and cervical dentine defects. Ground sections of the teeth cut longitudinally were stained with basic fuchsin and observed by fluorescence and confocal microscopy as well as transmitted light microscopy. Basic fuchsin stained the dentinal tubules, interglobular dentine and the granular layer of Tomes. These structures appeared intense blue to faint violet with transmitted light microscopy, whereas their staining displayed intense fluorescence with fluorescence microscopy. Therefore, the interglobular dentine could be detected more sensitively with fluorescence and confocal microscopy than with transmitted light microscopy. Typical interglobular dentine was present in coronal dentine in most of the teeth. In the radicular dentin, position and size of the interglobular dentine was different among the teeth examined. Most of the teeth had the interglobular dentine in the cervical part of the roots (type A). Two premolars displayed the interglobular dentine in the coronal half of the root (type B). The types A and B contained large interglobular areas. A small amount of interglobular dentine was restricted to the apical half of the roots of two canines and one molar (type C). In contrast to types A and B which were seen at both labial or buccal and lingual sides of roots, the interglobular dentine of type C was seen only at one side, labial or lingual. Some of the tooth roots did not show any interglobular dentine (type D). Most of the incisors, canines and premolar were types A, B, and C, respectively, and the molars were mixed types A, C, and D. These results suggest that the factors affecting dentinogenesis during root formation are unique for each tooth.

Keywords:
Interglobular dentine, Tooth roots, human, Fuchsin, basic, Fluorescence microscopy
1.
Appleton, J. (1991) The effect of lead acetate on dentine formation in the rat. Arch Oral Biol 36: 377–382.
2.
Appleton J. (1993) The structure of dentine after the injection of strontium chloride by backscattered electron imaging in the scanning electron microscope. Arch Oral Biol 38: 1–4.
3.
Appleton, J. (1994) Formation and structure of dentine in the rat incisor after chronic exposure to sodium fluoride. Scanning Microsc 8: 711–719.
4.
Avery, J.K. (1980) Dentine; in Bhaskar SN (ed): Orban’s Oral Histology and Embryology, ed 9. London, Mosby, pp 107–140.
5.
Bronckers, A.L., D.M. Lyaruu, J.H. Woltens (1989) Immunohistochemistry of extracellular matrix proteins during various stages of dentinogenesis. Connect Tissue Res 22: 65–70.
6.
Butler, W.T. (1995) Dentin matrix proteins and dentinogenesis. Connect Tissue Res 33: 59–65.
7.
Butler W.T. (1998) Dentin matrix proteins. Eur J Oral Sci 106(suppl 1):204–210.
8.
Butler W.T., H. Richie (1995) The nature and functional significance of dentin extracellular matrix protiens. Int J Dev Biol 39: 169–179.
9.
Dahl, J.E. (1984) Effects of methylmercury chloride on rat incisor odontoblasts and dentinogenesis. Acta Odontol Scand 42: 251–255.
10.
Fong, C.D., I. Slaby, L. Hammarstrom (1996) Amelin: An enamel-related protein, transcribed in the cells of epithelial root sheath. J Bone Miner Res 11: 892–898.
11.
Fujita H., T. Fujita (1992) Tooth; in Textbook of Histology Part 2, ed 3. Tokyo, Igaku-Shoin pp 83–103.
12.
Gente, M., E. Matthaei, P. Mayr, V. Schwarzmann (1989) Enamel and dentin under the ultrasonic microscope. Dtsch Zahnärztl Z 44: 56–58.
13.
Hietala, E.L., M.A. Larmas (1991) Mineral content of different areas of human dentin in hypophosphataemic vitamin D-resistant rickets. J Biol Buccale 19: 129–134.
14.
Kagayama, M., J.X. Zhu, Y. Sasano, H. Sato, H. Mayanagi (1997) Development of interglobular dentine in rat molars and its relation to maturation of enamel. Anat Embryol 196: 477–483.
15.
Kline, L.W., N.R. Thomas (1977) The role of calcitonin in the calcification of dental matrix. J Dent Res 56: 862–865.
16.
Limeback, H., C. Schlumbohm, A. Sen, G. Nikiforuk (1992) The effects of hypocalcemia/hypophosphatemia on porcine bone and dental hard tissues in an inherited form of type 1 pseudo-vitamin D deficiency rickets. J Dent Res 71: 346–352.
17.
Linde, A. (1989) Dentin matrix protiens: Composition and possible functions in calcification. Anat Rec 224: 154–166.
18.
Masatomi, Y., Y. Nakagawa, Y. Kanamoto, S. Sobue, T. Ooshima (1996) Effects of serum phosphate level on formation of incisor dentine in hypophosphatemic mice. J. Oral Pathol Med 25: 182–187.
19.
Matthiessen, M.E., B. Sogaard-Pedersen, P. Romert (1985) Electron microscopic demonstration of non-mineralized and hypomineralized areas in dentin and cementum by silver methenamine staining of collagen. Scand J Dent Res 93: 385–395.
20.
Mjör, I.A. (1966) Microradiography of human coronal dentine. Arch Oral Biol 11: 225–234.
21.
Piesco, N.P. (1994) Histology of dentin; in Avery JK (ed): Oral Development and Histology. New York Thieme Medical Publishers, pp 242–261.
22.
Pihlman K., E. Linder (1983) Fluorescence microscopical visualization of elastic fibres using basic fuchsin. Histochemistry 79: 157–165.
23.
Robey P.G. (1996) Vertebrate mineralized matrix proteins: Structure and function. Connect Tissue Res 35: 131–136.
24.
Shackleford, J.M. (1971) The structure of Tomes’ granular layer in dog premolar teeth. Anat Rec 170: 357–363.
25.
Symons, N.B.B. (1965) Interglobular dentine and the calcospherite pattern. Arch Oral Biol 10: 1009–1010.
26.
Zaazou, A.M., N. el-Masry (1973) The interglobular spaces in the dentin in the root portion and its relation to the granular layer of tomes. Egypt Dent J 19: 173–180.
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2000
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