... (Isopropyl β-d-1-thiogalactopyranoside), centrifuged, and sonicated. The supernatant, containing the soluble form of the protein, was subjected to immobilized metal affinity chromatography. The purified protein was quantified by the bicinchoninic acid assay and kept at −20°C before use. The saliva...

... components representing mineral content, protein content, porphyrin fluorescence (PF), putative infected dentine signal (IDS) and affected dentine signal (ADS). The distributions of the 5 Raman components and the AF signal were matched across all samples and their average values were calculated for each...

...A.J. White; L.H. Gracia; M.E. Barbour The application of milk-derived proteins such as casein as anti-erosion agents in oral healthcare products is of current interest. The aim of this study was to investigate the potential of 3 commercially available, milk-derived proteins as agents to inhibit...

... collected from the permanent dentition were directly related to concentrations in stimulated whole saliva of 45 males aged 12–13 years. The calcium, inorganic phosphorus, water-soluble carbohydrate and protein concentrations of free smooth surface plaque were related to both the time since the last reported...

...C. Robinson; J. Kirkham; J.A. Weatherell; A. Richards; K. Josephsen; O. Fejerskov Calcium, phosphorus and protein analyses have been performed on developing permanent enamel from the mandibular dentition of the domestic pig. The pattern of mineralization and protein loss was similar from tooth...

...W. Distler; A. Petschelt; A. Kröncke A very sensitive method for protein determination by means of o -phthalaldehyde was developed. Plaque wet weight is not suitable for standardizing small plaque samples, but protein content of plaque can be used to measure plaque quantity precisely. 18 11...

...J.C. Voegel; A. Belcourt; S. Gillmeth Dissolution in an acidic buffer of synthetic hydroxyapatites covered with various amounts of proteins (0–482.5 μg/ml·m 2 ) and treated with different quantities of fluoride (0–10 ppm of F –– ) was studied. Analyses before and after protein coating were made...

... profile of the matrix proteins of dental plaque showed distinct patterns when it was formed in the absence or presence of the different carbohydrates. Although the results suggest that the high cariogenicity of dental plaque formed in the presence of sucrose can be mainly explained by the high...