A new acid etch enamel microsampling technique was employed to study the effect of artificially produced acquired pellicle on fluoride uptake. The results showed less variance of parts per million fluoride and shallower depth than previously reported acid etch methods. The results of 120 enamel microsamplings showed that all enamel blocks receiving an acidulated fluoride treatment gained significant amounts of fluoride, with the highest concentration found in the groups not exposed to artificial pellicle. No significant difference in fluoride uptake was found between the group brushed with water after pellicle formation and the group which received a pumice prophylaxis after pellicle formation. Fluoride binding to salivary proteins was investigated to determine whether it could be a factor in fluoride measurements of surface enamel and whether the binding might decrease the amount of fluoride reaching the enamel. Salivary residues deposited on Millipore filters showed no measurable binding of fluoride at NaF range from 2 × 10––6 to 2 × 10––3 molar.

Keywords:
Enamel, Fluoride uptake, Pellicle
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© 1975 S. Karger AG, Basel
1975
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