Abstract
Washed cells of the FA-1 and K-1 strains of Streptococcus mutans were incubated in buffered solutions, pH 5.9, that were initially either saturated or slightly undersaturated by adding enamel granules. In the presence of enamel in the system, the total cell phosphorus of the nonfermenting cells was higher than in the absence of enamel. This change was reduced by fluoride and a fluoride-propanol combination. When 32P-labelled cells of Strep. mutans were introduced into the above system, a part of the 32P of bacterial origin was found in the enamel. Fluoride alone and in particular fluoride together with propanol or chlorhexidine increased the uptake of bacterial 32P by the enamel. During prolonged incubation of bacteria with softened enamel, the fluoride, especially together with chlorhexidine, caused a marked rehardening of the enamel surface.