The effect of changing the pH on the steady-state e.m.f.s. of 0.10/enamel/0.01M KCl concentration cells has been examined at 25 °C over the range pH 4.0–5.7 with potassium acetate-acetic acid buffers. Measurements were carried out with the potassium acetate concentration held at 0.002 m, at 0.0002 m, and a third series with an undissociated acetic acid concentration of 0.001 M. When the KAc concentration was constant the membrane potentials diminished as the pH was lowered and approached zero with 0.002 M KAc between pH 4.0 and 4.3. A smaller change was observed with the undissociated HAc concentration held at 0.001 M. It is inferred that, contrary to an earlier interpretation by Klein, the observed effect is mainly due to the concentration of undissociated acetic acid in solution. The possibility that the results may be explained in terms of the absorption of calcium ions produced by dissolution at the enamel and dentine interfaces is discussed.

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