Abstract
This study evaluated the combination of a sugarcane cystatin (CaneCPI-5) and sodium fluoride (NaF) in acquired pellicle engineering for the prevention of dental erosion in vitro. Seventy-five human enamel specimens were prepared and divided into 5 treatment groups (n = 15/group): Deionized water (Control); Elmex™ (SnCl2/NaF/AmF); 0.1 mg/mL CaneCPI-5; 500 ppm NaF; and CaneCPI-5+NaF (Combination). The specimens were individually treated (200 μL; 2 min; 37°C), then incubated in human saliva (200 μL; 1 h, at 37°C) for acquired pellicle formation. Afterward, the specimens were submitted to an erosive challenge (1% citric acid [CR], pH 3.6, 10 mL, 2 min, 25 °C). This sequence was conducted 5 times. Percentage of surface microhardness change (%SMC), relative surface reflection intensity (rSRI), and calcium released to the CR were measured and analyzed by one-way ANOVA followed by Tukey’s test (p < 0.05). In general, all the treatments (SnCl2/NaF/AmF, CaneCPI-5, NaF, and Combination) significantly protected the enamel when compared the control group. Regarding %SMC and rSRI, the Combination was the most effective treatment, reducing the %SMC significantly (p < 0.01) when compared to all the other treatments, although this difference was not significant in the CR analysis. All treatments demonstrated a protective effect on enamel against dental erosion; however, the combination of CaneCPI-5 with NaF showed a greater protection.
References
1.
Algarni
AA
, Mussi
MC
, Moffa
EB
, Lippert
F
, Zero
DT
, Siqueira
WL
, The impact of stannous, fluoride ions and its combination on enamel pellicle proteome and dental erosion prevention
. PLoS One
. 2015
;10
(6
):0128196
.2.
Babcock
FD
, King
JC
, Jordan
TH
. The reaction of stannous fluoride and hydroxyapatite
. J Dent Res
. 1978
;57
(9–10
):933
–8
. .3.
Baig
AA
, Faller
RV
, Yan
J
, Ji
N
, Lawless
M
, Eversole
SL
. Protective effects of SnF2: part I. Mineral solubilisation studies on powdered apatite
. Int Dent J
. 2014
;64
(Suppl 1
):4
–10
. .4.
Barrett
AJ
. The cystatins: a diverse superfamily of cysteine peptidase inhibitors
. Biomed Biochim Acta
. 1986
;45
(1112
):1363
–74
.5.
Brevik
SC
, Lussi
A
, Rakhmatullina
E
. A new optical detection method to assess the erosion inhibition by in vitro salivary pellicle layer
. J Dent
. 2013
;41
(5
):428
–35
. .6.
Buzalaf
MA
, Hannas
AR
, Kato
MT
. Saliva and dental erosion
. J Appl Oral Sci
. 2012
;20
(5
):493
–502
. .7.
Carvalho
TS
, Araújo
TT
, Ventura
TMO
, Dionizio
A
, Câmara
JVF
, Moraes
SM
, Acquired pellicle protein-based engineering protects against erosive demineralization
. J Dent
. 2020
;102
:103478
.8.
Carvalho
TS
, Baumann
T
, Lussi
A
. A new hand-held optical reflectometer to measure enamel erosion: correlation with surface hardness and calcium release
. Sci Rep
. 2016a
;6
:25259
. .9.
Carvalho
TS
, Baumann
T
, Lussi
A
. Does erosion progress differently on teeth already presenting clinical signs of erosive tooth wear than on sound teeth? An in vitro pilot trial
. BMC Oral Health
. 2016b
;17
(1
):14
. .10.
Cheaib
Z
, Lussi
A
. Impact of acquired enamel pellicle modification on initial dental erosion
. Caries Res
. 2011
;45
(2
):107
–12
. .11.
Cheaib
Z
, Rakmathulina
E
, Lussi
A
, Eick
S
. Impact of acquired pellicle modification on adhesion of early colonizers
. Caries Res
. 2015
;49
(6
):626
–32
. .12.
Delecrode
TR
, Siqueira
WL
, Zaidan
FC
, Bellini
MR
, Moffa
EB
, Mussi
MC
, Identification of acid-resistant proteins in acquired enamel pellicle
. J Dent
. 2015
;43
(12
):1470
–5
.13.
de Souza
BM
, Santi
LRP
, de Souza Silva
M
, Buzalaf
MAR
, Magalhães
AC
. Effect of an experimental mouth rinse containing NaF and TiF4 on tooth erosion and abrasion in situ
. J Dent
. 2018
;73
:45
–9
. .14.
de Souza
BM
, Santi
LRP
, João-Souza
SH
, Carvalho
TS
, Magalhães
AC
. Effect of titanium tetrafluoride/sodium fluoride solutions containing chitosan at different viscosities on the protection of enamel erosion in vitro
. Arch Oral Biol
. 2020
;120
:104921
. .15.
Emekli-Alturfan
E
, Yarat
A
, Çalışkan-Ak
E
, Pisiriciler
R
, Kuru
B
, Noyan
Ü
. Determination of storage time of saliva samples obtained from patients with and without chronic periodontitis for the comparison of some biochemical and cytological parameters
. J Clin Lab Anal
. 2013
;27
(4
):261
–6
. .16.
Ganss
C
, Klimek
J
, Schwarz
N
. A comparative profilometric in vitro study of the susceptibility of polished and natural human enamel and dentine surfaces to erosive demineralization
. Arch Oral Biol
. 2000
;45
(10
):897
–902
. .17.
Huysmans
MC
, Young
A
, Ganss
C
. The role of fluoride in erosion therapy
. Monogr Oral Sci
. 2014
;25
:230
–43
. .18.
Ionta
FG
, Bassoto
MAG
, Santos
NM
, Campli
FD
, Honório
HM
, Cruvinel
T
, Effect of in situ aspartame mouthwash to prevent intrinsic and extrinsic erosive tooth wear
. J Clin Exp Dent
. 2020
;12
(7
):638
–43
.19.
Jager
DH
, Vieira
AM
, Ligtenberg
AJ
, Bronkhorst
E
, Huysmans
MC
, Vissink
A
. Effect of salivary factors on the susceptibility of hydroxyapatite to early erosion
. Caries Res
. 2011
;45
(6
):532
–7
. .20.
Konradsson
K
, Lingström
P
, Emilson
CG
, Johannsen
G
, Ramberg
P
, Johannsen
A
. Stabilized stannous fluoride dentifrice in relation to dental caries, dental erosion and dentin hypersensitivity: a systematic review
. Am J Dent
. 2020
;33
(2
):95
–105
.21.
Lussi
A
, Bossen
A
, Höschele
C
, Beyeler
B
, Megert
B
, Meier
C
, Effects of enamel abrasion, salivary pellicle, and measurement angle on the optical assessment of dental erosion
. J Biomed Opt
. 2012
;17
(9
):97009
.22.
Lussi
A
, Buzalaf
MAR
, Duangthip
D
, Anttonen
V
, Ganss
C
, João-Souza
SH
, The use of fluoride for the prevention of dental erosion and erosive tooth wear in children and adolescents
. Eur Arch Paediatr Dent
. 2019
;20
(6
):517
–27
.23.
Marinho
VC
, Chong
LY
, Worthington
HV
, Walsh
T
. Fluoride mouthrinses for preventing dental caries in children and adolescents
. Cochrane Database Syst Rev
. 2016
;7
(7
):CD002284
. .24.
Martignon
S
, Bartlett
D
, Manton
DJ
, Martinez-Mier
EA
, Splieth
C
, Avila
V
. Epidemiology of erosive tooth wear, dental fluorosis and molar incisor hypomineralization in the American Continent
. Caries Res
. 2021
;55
(1
):1
–11
. .25.
Niemeyer
SH
, Baumann
T
, Lussi
A
, Meyer-Lueckel
H
, Scaramucci
T
, Carvalho
TS
. Salivary pellicle modification with polyphenol-rich teas and natural extracts to improve protection against dental erosion
. J Dent
. 2021
;105
:103567
. .26.
Pelá
VT
, Buzalaf
MAR
, Niemeyer
SH
, Baumann
T
, Henrique-Silva
F
, Toyama
D
, Acquired pellicle engineering with proteins/peptides: mechanism of action on native human enamel surface
. J Dent
. 2021a
;107
:103612
.27.
Pelá
VT
, Lunardelli
JGQ
, Tokuhara
CK
, Gironda
CC
, da Silva
NDG
, Carvalho
TS
, Safety and in situ antierosive effect of caneCPI-5 on dental enamel
. J Dent Res
. 2021b
. Epub ahead of print.28.
Pelá
VT
, Lunardelli
JGQ
, Ventura
TMO
, Camiloti
GD
, Baumann
T
, Carvalho
TS
, Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ or in vivo
. Eur J Oral Sc
. 2020
;128
(6
):487
–94
.29.
Rehage
M
, Delius
J
, Hofmann
T
, Hannig
M
. Oral astringent stimuli alter the enamel pellicle's ultrastructure as revealed by electron microscopy
. J Dent
. 2017
;63
:21
–9
. .30.
Santiago
AC
, Khan
ZN
, Miguel
MC
, Gironda
CC
, Soares-Costa
A
, Pelá
VT
, A new sugarcane cystatin strongly binds to dental enamel and reduces erosion
. J Dent Res
. 2017
;96
(9
):1051
–7
.31.
Schlueter
N
, Amaechi
BT
, Bartlett
D
, Buzalaf
MAR
, Carvalho
TS
, Ganss
C
, Terminology of erosive tooth wear: consensus report of a workshop organized by the ORCA and the Cariology Research Group of the IADR
. Caries Res
. 2020
;54
(1
):2
–6
.32.
Schlueter
N
, Hara
A
, Shellis
RP
, Ganss
C
. Methods for the measurement and characterization of erosion in enamel and dentine
. Caries Res
. 2011
;45
(Suppl 1
):13
–23
. .33.
Schlueter
N
, Hardt
M
, Lussi
A
, Engelmann
F
, Klimek
J
, Ganss
C
. Tin-containing fluoride solutions as anti-erosive agents in enamel: an in vitro tin-uptake, tissue-loss, and scanning electron micrograph study
. Eur J Oral Sci
. 2009
;117
(4
):427
–34
. .34.
Schlueter
N
, Klimek
J
, Ganss
C
. Effect of stannous and fluoride concentration in a mouth rinse on erosive tissue loss in enamel in vitro
. Arch Oral Biol
. 2009a
;54
(5
):432
–6
. .35.
Schlueter
N
, Klimek
J
, Ganss
C
. In vitro efficacy of experimental tin- and fluoride-containing mouth rinses as anti-erosive agents in enamel
. J Dent
. 2009b
;37
(12
):944
–8
. .36.
Siqueira
WL
, Bakkal
M
, Xiao
Y
, Sutton
JN
, Mendes
FM
. Quantitative proteomic analysis of the effect of fluoride on the acquired enamel pellicle
. PLoS One
. 2012
;7
(8
):e42204
. .37.
Soares-Costa
A
, Beltramini
LM
, Thiemann
OH
, Henrique-Silva
F
. A sugarcane cystatin: recombinant expression, purification, and antifungal activity
. Biochem Biophys Res Commun
. 2002
;296
(5
):1194
–9
. .38.
Ten Cate
JM
, Buzalaf
MAR
. Fluoride mode of action: once there was an observant dentist
. J Dent Res
. 2019
;98
(7
):725
–30
.39.
Wachtel
LW
. In vitro comparison effercts of topical stannic fluoride and stannous fluoride solutions on enamel solubility
. Arch Oral Biol
. 1964 Jul–Aug
;9
:439
–45
. .40.
Weber
MT
, Hannig
M
, Pötschke
S
, Höhne
F
, Hannig
C
. Application of plant extracts for the revention of dental erosion: an in situ/in vitro Study
. Caries Res
. 2015
;49
(5
):477
–87
. .41.
West
X
, Addy
M
, Newcombe
R
, Macdonald
E
, Chapman
A
, Davies
M
, A randomised crossover trial to compare the potential of stannous fluoride and essential oil mouth rinses to induce tooth and tongue staining
. Clin Oral Investig
. 2012
;16
(3
):821
–6
.42.
Zanatta
RF
, Caneppele
TMF
, Scaramucci
T
, Dib
RE
, Maia
LC
, Ferreira
DMTP
, Protective effect of fluorides on erosion and erosion/abrasion in enamel: a systematic review and meta-analysis of randomized in situ trials
. Arch Oral Biol
. 2020
;120
:104945
.43.
Zimmermann
R
, Delius
J
, Friedrichs
J
, Stehl
S
, Hoffnann
T
, Hannig
C
, Impact of oral astringent stimuli on surface charge and morphology of the protein-rich pellicle at the tooth-saliva interphase
. Colloids Surf B
. 2019
;174
:451
–8
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