Abrasives may counteract the efficacy of anti-erosion toothpastes either due to physical effects or due to interaction with active agents. This study aimed to investigate whether the amount of abrasives is a determinant for the efficacy of Sn2+-containing toothpastes with or without chitosan additive. Enamel samples were eroded (0.50 wt% citric acid, pH 2.5; 6 × 2 min/day) on a shaking desk - 30/min in experiment 1 (E1) and 35/min in experiments 2 (E2) and 3 (E3) - and immersed in toothpaste slurries (2 × 2 min). Half of the samples were additionally brushed (15 s, load 200 g) within the immersion time. The toothpastes contained 0, 5, 10, 15, and 20% silica. In E1 and E2 the active ingredients were F- (700 ppm as amine fluoride, 700 ppm as NaF) and Sn2+ (3,500 ppm as SnCl2); in E3 chitosan (0.5%) was additionally added. The placebo contained 20% silica. Tissue loss was determined profilometrically. In E1, slurries completely inhibited tissue loss; distinct surface deposits occurred. With brushing, tissue loss significantly increased up to an abrasive content of 10%, but decreased significantly with higher amounts; 20% silica revealed similar values as the abrasive-free formulation. In E2, all slurries inhibited tissue loss distinctly irrespective of the amounts of abrasives. With brushing, a similar trend as in E1 was observed but with much less efficacy. The chitosan-containing formulations in E3 were much more effective; similar results as in E1 were found. In conclusion, the amount of abrasives had no effect when toothpastes were applied as slurries, but played an important role with brushing.

Azadi-Schossig P, Becker K, Attin T: Chelating effect of citric acid is negligible for development of enamel erosions. Clin Oral Investig 2016;20:1577-1587.
Claesson PM, Ninham BW: pH-dependent interactions between adsorbed chitosan layers. Langmuir 1992;8:1406-1412.
Comar LP, Gomes MF, Ito N, Salomao PA, Grizzo LT, Magalhaes AC: Effect of NaF, SnF2, and TiF4 toothpastes on bovine enamel and dentin erosion-abrasion in vitro. Int Dent J 2012;2012:134350.
Cong WL, Zhang PF, Pei ZJ: Experimental investigations on material removal rate and surface roughness in lapping of substrate wafers: a literature review. Key Eng Mater 2009;404:23-31.
Dedinaite A, Lundin M, Macakova L, Auletta T: Mucin-chitosan complexes at the solid-liquid interface: multilayer formation and stability in surfactant solutions. Langmuir 2005;21:9502-9509.
Faller RV, Eversole SL, Tzeghai GE: Enamel protection: a comparison of marketed dentifrice performance against dental erosion. Am J Dent 2011;24:205-210.
Ganss C, Lussi A, Grunau O, Klimek J, Schlueter N: Conventional and anti-erosion fluoride toothpastes: effect on enamel erosion and erosion-abrasion. Caries Res 2011;45:581-589.
Ganss C, Lussi A, Schlueter N: Dental erosion as oral disease: insights in etiological factors and pathomechanisms, and current strategies for prevention and therapy. Am J Dent 2012a;25:251-264.
Ganss C, Schulze K, Schlueter N: Toothpaste and erosion. Monogr Oral Sci 2013;23:88-99.
Ganss C, von Hinckeldey J, Tolle A, Schulze K, Klimek J, Schlueter N: Efficacy of the stannous ion and a biopolymer in toothpastes on enamel erosion/abrasion. J Dent 2012b;40:1036-1043.
Gerrard WA, Winter PJ: Evaluation of toothpastes by their ability to assist rehardening of enamel in vitro. Caries Res 1986;20:209-216.
Hara AT, Gonzales-Cabezas C, Creeth J, Parmar M, Eckert GJ, Zero DT: Interplay between fluoride and abrasivity of dentifrices on dental erosion-abrasion. J Dent 2009;37:781-785.
Hooper SM, Newcombe RG, Faller R, Eversole S, Addy M, West NX: The protective effects of toothpaste against erosion by orange juice: studies in situ and in vitro. J Dent 2007;35:476-481.
Kampf N, Raviv U, Klein J: Normal and shear forces between adsorbed and gelled layers of Chitosan, a naturally occurring cationic polyelectrolyte. Macromolecules 2004;37:1134-1142.
Lundin M, Macakova L, Dedinaite A, Claesson P: Interactions between chitosan and SDS at a low-charged silica substrate compared to interactions in the bulk - the effect of ionic strength. Langmuir 2008;24:3814-3827.
Marinho VC, Higgins JP, Logan S, Sheiham A: Fluoride varnishes for preventing dental caries in children and adolescents. Cochrane Database Syst Rev 2013;7:CD002279.
Raviv U, Giasson S, Kampf N, Gohy J-F, Jerome R, Klein R: Lubrication by charged polymers. Nature 2003;425:163-165.
Schlueter N, Hardt M, Lussi A, Engelmann F, Klimek J, Ganss C: Tin-containing fluoride solutions as anti-erosive agents in enamel: an in vitro tin-uptake, tissue-loss and scanning electron micrograph study. Eur J Oral Sci 2009;117:427-434.
Schlueter N, Klimek J, Ganss C: Effect of a chitosan additive to a Sn2+-containing toothpaste on its anti-erosive/anti-abrasive efficacy - a controlled randomised in situ trial. Clin Oral Investig 2014;18:107-115.
Shellis RP, Finke M, Eisenburger M, Parker DM, Addy M: Relationship between enamel erosion and liquid flow rate. Eur J Oral Sci 2005;113:232-238.
Svensson O, Lingh L, Cardenas M, Arnebrant T: Layer-by-layer assembly of mucin and chitosan - influence of surface properties, concentration and type of mucin. J Colloid Interface Sci 2006;299:608-616.
van der Mei HC, Engels E, de Vries J, Dijkstra RJ, Busscher HJ: Chitosan adsorption to salivary pellicles. Eur J Oral Sci 2007;115:303-307.
Voronets J, Lussi A: Thickness of softened human enamel removed by toothbrush abrasion: an in vitro study. Clin Oral Invest 2010;14:251-256.
Wiegand A, Attin T: Design of erosion/abrasion studies - insights and rational concepts. Caries Res 2011;45:53-59.
Wiegand A, Magalhaes AC, Attin T: Is titanium tetrafluoride (TiF4) effective to prevent carious and erosive lesions? A review of the literature. Oral Health Prev Dent 2010;8:159-164.
Wiegand A, Schwerzmann M, Sener B, Magalhaes AC, Roos M, Ziebolz D, Imfeld T, Attin T: Impact of toothpaste slurry abrasivity and toothbrush filament stiffness on abrasion of eroded enamel - an in vitro study. Acta Odontol Scand 2008;66:231-235.
Young A, Thrane PS, Saxegaard E, Jonski G, Rölla G: Effect of stannous fluoride toothpaste on erosion-like lesions: an in vivo study. Eur J Oral Sci 2006;114:180-183.
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