Profilometry is established in erosion research. However, in the case of dentine, factors such as the demineralised organic matrix, desiccation effects, or type of measuring device may have an impact on the measurement results, which were investigated in the present study. Dentine specimens were eroded with citric acid (1%, pH 2.6) for 5, 10, 15, 20, 30, 60, 90, and 120 min (n = 15 each). For each specimen, tissue loss was determined under various conditions - before/after enzymatic matrix removal, under standardised wet and desiccated (2/10 min) conditions - with non-contact and contact profilometry. In the presence of matrix, under wet conditions, non-contact profilometry revealed almost no tissue loss. Values (mean ± SD) ranged between 0.3 ± 0.7 µm (5 min) and 3.4 ± 1.5 µm (120 min). Contact profilometry increased values significantly (range: 2.9 ± 1.1 to 30.6 ± 5.8 µm). Desiccation (2 min) significantly increased values, except for 5 min of demineralisation, for non-contact profilometry (range: 0.8 ± 1.3 to 22.1 ± 5.5 µm), and decreased values for contact profilometry up to 15 min and increased them as from 90 min (range: 0.9 ± 1.2 to 33.0 ± 5.5 µm); results after 10 min of desiccation were comparable. After the removal of matrix, under wet conditions, values were distinctly higher (non-contact: 3.5 ± 0.8-55.5 ± 7.4 µm; contact: 4.2 ± 1.3-57.8 ± 8.1 µm). Desiccation (10 min) lowered values by about 2-5 µm due to specimen deformation. Bland-Altman comparisons of various outcomes revealed distinct significant proportional and relative biases. Loss of mineralised tissue cannot be adequately quantified in the presence of matrix. Desiccation leads to matrix shrinkage and specimen deformation. Most importantly, tissue loss values obtained in the presence or absence of matrix are not proportional. Therefore, if mineral status is the target criterion, matrix removal and moisture control are prerequisites.

Attin T, Becker K, Roos M, Attin R, Paqué F: Impact of storage conditions on profilometry of eroded dental hard tissue. Clin Oral Invest 2009;13:473-478.
Attin T, Wegehaupt FJ: Methods for assessment of dental erosion. Monogr Oral Sci 2014;25:123-142.
Barbour ME, Rees JS: The laboratory assessment of enamel erosion: a review. J Dent 2004;32:591-602.
Bland JM, Altman DG: Applying the right statistics: analyses of measurement studies. Ultrasound Obstet Gynecol 2003;22:85-93.
Flury S, Koch T, Peutzfeldt A, Lussi A, Ganss C: The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin. Clin Oral Invest 2013;17:217-225.
Ganss C, Hardt M, Blazek D, Klimek J, Schlueter N: Effects of toothbrushing force on the mineral content and demineralized organic matrix of eroded dentine. Eur J Oral Sci 2009a;117:255-260.
Ganss C, Hardt M, Lussi A, Cocks A-K, Klimek J, Schlueter N: Mechanism of action of tin-containing fluoride solutions as anti-erosive agents in dentine - an in vitro tin-uptake, tissue loss and SEM-study. Eur J Oral Sci 2010;118:376-384.
Ganss C, Klimek J, Starck C: Quantitative analysis of the impact of the organic matrix on the fluoride effect on erosion progression in human dentine using longitudinal microradiography. Arch Oral Biol 2004;49:931-935.
Ganss C, Lussi A, Scharmann I, Weigelt T, Hardt M, Klimek J, et al: Comparison of calcium analysis, longitudinal microradiography and profilometry for the quantitative assessment of erosion in dentine. Caries Res 2009b;43:422-429.
Ganss C, Lussi A, Schlueter N: The histological features and physical properties of eroded dental hard tissues. Monogr Oral Sci 2014;25:99-107.
Ganss C, Schlueter N, Hardt M, von Hinckeldey J, Klimek J: Effects of toothbrushing on eroded dentine. Eur J Oral Sci 2007;115:390-396.
Gerrard WA, Winter PJ: Evaluation of toothpastes by their ability to assist rehardening of enamel in vitro. Caries Res 1986;20:209-216.
Hara AT, Karlinsey RL, Zero DT: Dentine remineralization by simulated saliva formulations with different Ca and Pi contents. Caries Res 2008;42:51-56.
Kato MT, Leite AL, Hannas AR, Calabria MP, Magalhães AC, Pereira JC, et al: Impact of protease inhibitors on dentin matrix degradation by collagenase. J Dent Res 2012;91:1119-1123.
Kleter GA, Damen JJ, Everts V, Niehof J, ten Cate JM: The influence of the organic matrix on demineralization of bovine root dentin in vitro. J Dent Res 1994;73:1523-1529.
Ludbrook J: Statistical techniques for comparing measurers and methods of measurement: a critical review. Clin Exp Pharmacol Physiol 2002;29:527-536.
Lussi A, Schlueter N, Rakhmatullina E, Ganss C: Dental erosion - an overview with emphasis on chemical and histopathological aspects. Caries Res 2011;45(suppl 1):2-12.
Ruben J, Arends J: Shrinkage of sound and demineralized human coronal dentine slabs. Caries Res 1993;27:266-270.
Schlueter N, Glatzki J, Klimek J, Ganss C: Erosive-abrasive tissue loss in dentine under simulated bulimic conditions. Arch Oral Biol 2012;57:1176-1182.
Schlueter N, Hara AT, Shellis RP, Ganss C: Methods for measurement and characterization of erosion in enamel and dentine. Caries Res 2011;45(suppl 1):13-23.
Schlueter N, Hardt M, Klimek J, Ganss C: Influence of the digestive enzymes trypsin and pepsin in vitro on the progression of erosion in dentine. Arch Oral Biol 2010;55:294-299.
Schwendicke F, Felstehausen G, Carey C, Dörfer C: Comparison of four methods to assess erosive substance loss of dentin. PLoS One 2014;9:e108064.
Steiner-Oliveira C, Nobre-dos-Santos M, Zero DT, Eckert G, Hara AT: Effect of a pulsed CO2 laser and fluoride on the prevention of enamel and dentine erosion. Arch Oral Biol 2010;55:127-133.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.