The feasibility of colorimetric parameters for the discrimination of the stages of dental caries based on a light-induced autofluorescence spectrum at a 405-nm excitation wavelength was investigated. The fluorescence spectra of 4 groups of tooth samples (10 sound, 10 early-stage decay, 14 established decay, and 10 severe decay), which were classified by the International Caries Detection and Assessment System, were experimentally measured in vitro. The carious lesion samples had an additional fluorescence peak at around 627 nm. The mathematical relation of the fluorescence spectrum and human color perception was established and computed. With increasing severity, the fluorescence color changed from green to yellow according to the colorimetric parameters of the CIE 1931 (x, y) chromaticity coordinates and dominant wavelengths. The results from a one-way ANOVA of the dominant wavelength showed a statistically significant difference among the 4 classified groups. The colorimetric parameters of the light-induced fluorescence spectrum can potentially be applied to evaluate the various carious levels.

1.
Alammari MR, Smith PW, de Josselin de Jong E, Higham SM: Quantitative light-induced fluorescence (QLF): a tool for early occlusal dental caries detection and supporting decision making in vivo. J Dent 2013;41:127-132.
2.
Betrisey E, Rizcalla N, Krejci I, Ardu S: Caries diagnosis using light fluorescence devices: VistaProof and DIAGNOdent. Odontology 2014;102:330-335.
3.
Borisova E, Uzunov T, Avramov L: Laser-induced autofluorescence study of caries model in vitro. Lasers Med Sci 2006;21:34-41.
4.
De Benedetto MS, Morais CC, Novaes TF, de Almeida Rodrigues J, Braga MM, Mendes FM: Comparing the reliability of a new fluorescence camera with conventional laser fluorescence devices in detecting caries lesions in occlusal and smooth surfaces of primary teeth. Lasers Med Sci 2011;26:157-162.
5.
Ferreira ZA, Santiago E, Eckert G, Fontana M, Ando M, Zero DT: Use of ICDAS combined with quantitative light-induced fluorescence as a caries detection method. Caries Res 2010;44:317-322.
6.
Fried D, Xie J, Shafi S, Featherstone JD, Breunig TM, Le C: Imaging caries lesions and lesion progression with polarization sensitive optical coherence tomography. J Biomed Opt 2002;7:618-627.
7.
Gomez J, Zakian C, Salsone S, Pinto SC, Taylor A, Pretty IA, Ellwood R: In vitro performance of different methods in detecting occlusal caries lesions. J Dent 2013;41:180-186.
8.
Gómez-Polo C, Gómez-Polo M, Celemin-Viñuela A, Martínez Vázquez De Parga JA: Differences between the human eye and the spectrophotometer in the shade matching of tooth colour. J Dent 2014;42:742-745.
9.
Hintze H, Wenzel A, Danielsen B, Nyvad B: Reliability of visual examination, fibre-optic transillumination, and bite-wing radiography, and reproducibility of direct visual examination following tooth separation for the identification of cavitated carious lesions in contacting approximal surfaces. Caries Res 1998;32:204-209.
10.
Jablonski-Momeni A, Stucke J, Steinberg T, Heinzel-Gutenbrunner M: Use of ICDAS-II, fluorescence-based methods, and radiography in detection and treatment decision of occlusal caries lesions: an in vitro study. Int J Dent 2012;2012:371595.
11.
Johnston WM: Color measurement in dentistry. J Dent 2009;37(suppl 1):e2-e6.
12.
Ko AC, Choo-Smith LP, Hewko M, Sowa MG, Dong CC, Cleghorn B: Detection of early dental caries using polarized Raman spectroscopy. Opt Express 2006;14:203-215.
13.
Lussi A, Hibst R, Paulus R: DIAGNOdent: an optical method for caries detection. J Dent Res 2004;83:C80-C83.
14.
Panayotov I, Terrer E, Salehi H, Tassery H, Yachouh J, Cuisinier FJ, Levallois B: In vitro investigation of fluorescence of carious dentin observed with a Soprolife® camera. Clin Oral Investig 2013;17:757-763.
15.
Pitts N: ICDAS - an international system for caries detection and assessment being developed to facilitate caries epidemiology, research and appropriate clinical management. Community Dent Health 2004;21:193-198.
16.
Sufyan G, Lippo L, Marwa H, Muneim S, Lugane B, Ziad S, Pekka V: Influence of staining solutions and whitening procedures on discoloration of hybrid composite resins. Acta Odontol Scand 2013;71:144-150.
17.
Thomas SS, Jayanthi JL, Subhash N, Thomas J, Mallia RJ, Aparna GN: Characterization of dental caries by LIF spectroscopy with 404-nm excitation. Lasers Med Sci 2011;26:299-305.
18.
Thomas SS, Mohanty S, Jayanthi JL, Varughese JM, Balan A, Subhash N: Clinical trial for detection of dental caries using laser-induced fluorescence ratio reference standard. J Biomed Opt 2010;15:027001.
19.
Walsh LJ, Shakibaie F: Ultraviolet-induced fluorescence: shedding new light on dental biofilms and dental caries. Australas Dent Pract 2007;18:56-60.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.