Dentin organic matrix, with type I collagen as the main component, is exposed after demineralization in dentinal caries, erosion or acidic conditioning during adhesive composite restorative treatment. This exposed matrix is prone to slow hydrolytic degradation by host collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins. Here we review the recent findings demonstrating that inhibition of salivary or dentin endogenous collagenolytic enzymes may provide preventive means against progression of caries or erosion, just as they have been shown to retain the integrity and improve the longevity of resin composite filling bonding to dentin. This paper also presents the case that the organic matrix in caries-affected dentin may not be preserved as intact as previously considered. In partially demineralized dentin, MMPs and cysteine cathepsins with the ability to cleave off the terminal non-helical ends of collagen molecules (telopeptides) may lead to the gradual loss of intramolecular gap areas. This would seriously compromise the matrix ability for intrafibrillar remineralization, which is considered essential in restoring the dentin's mechanical properties. More detailed data of the enzymes responsible and their detailed function in dentin-destructive conditions may not only help to find new and better preventive means, but better preservation of demineralized dentin collagenous matrix may also facilitate true biological remineralization for the better restoration of tooth structural and mechanical integrity and mechanical properties.

1.
Abbott PV: Assessing restored teeth with pulp and periapical diseases for the presence of cracks, caries and marginal breakdown. Aust Dent J 2004;49:33-39.
2.
Abdalla AI: Effect of long-term water aging on microtensile bond strength of self-etch adhesives to dentin. Am J Dent 2010;23:29-33.
3.
Arends J, Ruben J, Jongebloed WL: Dentine caries in vivo. Combined scanning electron microscopic and microradiographic investigation. Caries Res 1989;23:36-41.
4.
Armstrong SR, Vargas MA, Chung I, Pashley DH, Campbell JA, Laffoon JE, Qian F: Resin-dentin interfacial ultrastructure and microtensile dentin bond strength after five-year water storage. Oper Dent 2004;29:705-712.
5.
Balooch M, Habelitz S, Kinney JH, Marshall SJ, Marshall GW: Mechanical properties of mineralized collagen fibrils as influenced by demineralization. J Struct Biol 2008;162:404-410.
6.
Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ, Marshall GW Jr: Biomechanical perspective on the remineralization of dentin. Caries Res 2009;43:70-77.
7.
Bertassoni LE, Habelitz S, Marshall SJ, Marshall GW: Mechanical recovery of dentin following remineralization in vitro - an indentation study. J Biomech 2011;44:176-181.
8.
Bertassoni LE, Marshall GW: Papain-gel degrades intact nonmineralized type I collagen fibrils. Scanning 2009;31:253-258.
9.
Boukpessi T, Menashi S, Camoin L, ten Cate JM, Goldberg M, Chaussain-Miller C: The effect of stromelysin-1 (MMP-3) on non-collagenous extracellular matrix proteins of demineralized dentin and the adhesive properties of restorative resins. Biomaterials 2008;29:4367-4373.
10.
Boushell LW, Kaku M, Mochida Y, Bagnell R, Yamauchi M: Immunohistochemical localization of matrixmetalloproteinase-2 in human coronal dentin. Arch Oral Biol 2008;53:109-116.
11.
Boushell LW, Nagaoka H, Nagaoka H, Yamauchi M: Increased matrix metalloproteinase-2 and bone sialoprotein response to human coronal caries. Caries Res 2011;45:453-459.
12.
Brackett MG, Tay FR, Brackett WW, Dib A, Dipp FA, Mai S, Pashley DH: In vivo chlorhexidine stabilization of hybrid layers of an acetone-based dentin adhesive. Oper Dent 2009;34:379-383.
13.
Brackett WW, Tay FR, Brackett MG, Dib A, Sword RJ, Pashley DH: The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent 2007;32:107-111.
14.
Breschi L, Cammelli F, Visintini E, Mazzoni A, Vita F, Carrilho M, Cadenaro M, Foulger S, Tay FR, Pashley DH: Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-month in vitro study. J Adhes Dent 2009;11:191-198.
15.
Breschi L, Mazzoni A, Nato F, Carrilho M, Visintini E, Tjäderhane L, Ruggeri A Jr, Tay FR, Dorigo Ede S, Pashley DH: Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study. Dent Mater 2010;26:320-325.
16.
Buzalaf MA, Kato MT, Hannas AR: The role of matrix metalloproteinases in dental erosion. Adv Dent Res 2012;24:72-76.
17.
Buzalaf MA, Pessan JP, Honório HM, ten Cate JM: Mechanisms of action of fluoride for caries control. Monogr Oral Sci 2011;22:97-114.
18.
Carrilho MR: Can exogenous protease inhibitors control dentin matrix degradation? J Dent Res 2012;91:1099-1102.
19.
Carrilho MR, Carvalho RM, De Goes MF, Hipolito V, Geraldeli S, Tay FR, Pashley D, Tjäderhane L: Chlorhexidine preserves dentin bond in vitro. J Dent Res 2007a;86:90-94.
20.
Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjäderhane L, Reis AF, Hebling J, Mazzoni A, Breschi L, Pashley D: In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res 2007b;86:529-533.
21.
Carrilho MR, Tay FR, Donnelly AM, Agee KA, Tjäderhane L, Mazzoni A, Breschi L, Foulger S, Pashley DH: Host-derived loss of dentin matrix stiffness associated with solubilization of collagen. J Biomed Mater Res B Appl Biomater 2009;90:373-380.
22.
Charadram N, Farahani RM, Harty D, Rathsam C, Swain MV, Hunter N: Regulation of reactionary dentin formation by odontoblasts in response to polymicrobial invasion of dentin matrix. Bone 2012;50:265-275.
23.
Chaussain C, Boukpessi T, Khaddam M, Tjäderhane L, George A, Menashi S: Dentin matrix degradation by host matrix metalloproteinases: inhibition and clinical perspectives toward regeneration. Front Physiol 2013;4:308.
24.
Cooper PR, McLachlan JL, Simon S, Graham LW, Smith AJ: Mediators of inflammation and regeneration. Adv Dent Res 2011;23:290-295.
25.
Dayan D, Binderman I, Mechanic GL: A preliminary study of activation of collagenase in carious human dentine matrix. Arch Oral Biol 1983;28:185-187.
26.
Dechichi P, Biffi JC, Moura CC, de Ameida AW: A model of the early mineralization process of mantle dentin. Micron 2007;38:486-491.
27.
Demeule M, Brossard M, Pagé M, Gingras D, Béliveau R: Matrix metalloproteinase inhibition by green tea catechins. Biochim Biophys Acta 2000;1478:51-60.
28.
De Munck J, van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, Lambrechts P, Vanherle G: Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res 2003;82:136-140.
29.
Deyhle H, Bunk O, Müller B: Nanostructure of healthy and caries-affected human teeth. Nanomedicine 2011;7:694-701.
30.
Dickinson DP: Cysteine peptidases of mammals: their biological roles and potential effects in the oral cavity and other tissues in health and disease. Crit Rev Oral Biol Med 2002;13:238-275.
31.
Findeisen P, Neumaier M: Functional protease profiling for diagnosis of malignant disease. Proteomics Clin Appl 2012;6:60-78.
32.
Ganss C, Klimek J, Schäffer U, Spall T: Effectiveness of two fluoridation measures on erosion progression in human enamel and dentine in vitro. Caries Res 2001;35:325-330.
33.
Ganss C, Klimek J, Starck C: Quantitative analysis of the impact of the organic matrix on the fluoride effect on erosion progression in human dentine using longitudinal microradiography. Arch Oral Biol 2004;49:931-935.
34.
Garcia-Godoy F, Kramer N, Feilzer AJ, Frankenberger R: Long-term degradation of enamel and dentin bonds: 6-year results in vitro vs. in vivo. Dent Mater 2010;26:1113-1118.
35.
Garnero P, Borel O, Byrjalsen I, Ferreras M, Drake FH, McQueney MS, Foged NT, Delmas PD, Delaissé JM: The collagenolytic activity of cathepsin K is unique among mammalian proteinases. J Biol Chem 1998;273:32347-32352.
36.
Garnero P, Ferreras M, Karsdal MA, Nicamhlaoibh R, Risteli J, Borel O, Qvist P, Delmas PD, Foged NT, Delaissé JM: The type I collagen fragments ICTP and CTX reveal distinct enzymatic pathways of bone collagen degradation. J Bone Miner Res 2003;18:859-867.
37.
Gendron R, Grenier D, Sorsa T, Mayrand D: Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagn Lab Immunol 1999;6:437-439.
38.
Golub EE: Role of matrix vesicles in biomineralization. Biochim Biophys Acta 2009;1790:1592-1598.
39.
Hannas AR, Pereira JC, Granjeiro JM, Tjäderhane L: The role of matrix metalloproteinases in oral environment. Acta Odontol Scand 2007;65:1-13.
40.
Hashimoto M, Fujita S, Nagano F, Ohno H, Endo K: Ten-years degradation of resin-dentin bonds. Eur J Oral Sci 2010;118:404-410.
41.
Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H: In vivo degradation of resin-dentin bonds in humans over 1-3 years. J Dent Res 2000;79:1385-1391.
42.
Hebling J, Pashley DH, Tjäderhane L, Tay FR: Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005;84:741-746.
43.
Hedenbjörk-Lager A, Bjørndal L, Gustafsson A, Sorsa T, Tjäderhane L, Åkerman S, Ericson D: Caries correlates strongly to salivary levels of MMP-8. Caries Res 2015;49:1-8.
44.
Heintze SD, Ruffieux C, Rousson V: Clinical performance of cervical restorations - a meta-analysis. Dent Mater 2010;26:993-1000.
45.
Hickel R, Kaaden C, Paschos E, Buerkle V, García-Godoy F, Manhart J: Longevity of occlusally-stressed restorations in posterior primary teeth. Am J Dent 2005;18:198-211.
46.
Hietala EL, Tjäderhane L, Larmas M: Dentin caries recording with Schiff's reagent, fluorescence and back scattered electron image. J Dent Res 1993;72:1588-1592.
47.
Ito S, Saito T, Tay FR, Carvalho RM, Yoshiyama M, Pashley DH: Water content and apparent stiffness of non-caries versus caries-affected human dentin. J Biomed Mater Res B Appl Biomater 2005;72:109-116.
48.
Kafienah W, Brömme D, Buttle DJ, Croucher LJ, Hollander AP: Human cathepsin K cleaves native type I and II collagens at the N-terminal end of the triple helix. Biochem J 1998;331:727-732.
49.
Kato MT, Bolanho A, Zarella BL, Salo T, Tjäderhane L, Buzalaf MA: Sodium fluoride inhibits matrix MMP-2 and MMP-9. J Dent Res 2014;93:74-77.
50.
Kato MT, Leite AL, Hannas AR, Buzalaf MA: Gels containing MMP inhibitors prevent dental erosion in situ. J Dent Res 2010;89:468-472.
51.
Kato MT, Leite AL, Hannas AR, Calabria MP, Magalhães AC, Pereira JC, Buzalaf MA: Impact of protease inhibitors on dentin matrix degradation by collagenase. J Dent Res 2012;91:1119-1123.
52.
Kato MT, Magalhães AC, Rios D, Hannas AR, Attin T, Buzalaf MA: Protective effect of green tea on dentin erosion and abrasion. J Appl Oral Sci 2009;17:560-564.
53.
Katunuma N, Ohashi A, Sano E, Ishimaru N, Hayashi Y, Murata E: Catechin derivatives: specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels. FEBS Lett 2006;580:741-746.
54.
Khaddam M, Salmon B, Le Denmat D, Tjäderhane L, Menashi S, Chaussain C, Rochefort GY, Boukpessi T: Grape seed extracts inhibit dentin matrix degradation by MMP-3. Front Physiol 2014;5:425.
55.
Kinney JH, Balooch M, Haupt DL Jr, Marshall SJ, Marshall GW Jr: Mineral distribution and dimensional changes in human dentin during demineralization. J Dent Res 1995;74:1179-1184.
56.
Kinney JH, Habelitz S, Marshall SJ, Marshall GW: The importance of intrafibrillar mineralization of collagen on the mechanical properties of dentin. J Dent Res 2003;82:957-961.
57.
Kinney JH, Pople JA, Driessen CH, Breunig TM, Marshall GW, Marshall SJ: Intrafibrillar mineral may be absent in dentinogenesis imperfecta type II (DI-II). J Dent Res 2001;80:1555-1559.
58.
Kleter GA, Damen JJ, Everts V, Niehof J, ten Cate JM: The influence of the organic matrix on demineralization of bovine root dentin in vitro. J Dent Res 1994;73:1523-1529.
59.
Kuboki Y, Ohgushi K, Fusayama T: Collagen biochemistry of the two layers of carious dentin. J Dent Res 1977;56:1233-1237.
60.
Lauhio A, Salo T, Tjäderhane L, Lähdevirta J, Golub LM, Sorsa T: Tetracyclines in treatment of rheumatoid arthritis. Lancet 1995;346:645-646.
61.
López-Otín C, Overall CM: Protease degradomics: a new challenge for proteomics. Nat Rev Mol Cell Biol 2002;3:509-519.
62.
Magalhães AC, Wiegand A, Rios D, Hannas A, Attin T, Buzalaf MA: Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. J Dent 2009;37:994-998.
63.
Manfro AR, Reis A, Loguercio AD, Imparato JC, Raggio DP: Effect of different concentrations of chlorhexidine on bond strength of primary dentin. Pediatr Dent 2012;34:e11-e15.
64.
Marshall GW, Habelitz S, Gallagher R, Balooch M, Balooch G, Marshall SJ: Nanomechanical properties of hydrated carious human dentin. J Dent Res 2001;80:1768-1771.
65.
Martin-De Las Heras S, Valenzuela A, Overall CM: The matrix metalloproteinase gelatinase A in human dentine. Arch Oral Biol 2000;45:757-765.
66.
Mazzoni A, Breschi L, Carrilho M, Nascimento FD, Orsini G, Ruggeri A, Gobbi P, Mazzotti G, Tay FR, Pashley DH, Tjäderhane L: A review on nature, role and functions of dentin non-collagenous proteins. Part II: enzymes, serum proteins and growth factors. Endod Topics 2012a;21:19-40.
67.
Mazzoni A, Mannello F, Tay FR, Tonti GA, Papa S, Mazzotti G, Di Lenarda R, Pashley DH, Breschi L: Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. J Dent Res 2007;86:436-440.
68.
Mazzoni A, Nascimento FD, Carrilho M, Tersariol I, Papa V, Tjäderhane L, Di Lenarda R, Tay FR, Pashley DH, Breschi L: MMP activity in the hybrid layer detected with in situ zymography. J Dent Res 2012b;91:467-472.
69.
Mazzoni A, Papa V, Nato F, Carrilho M, Tjäderhane L, Ruggeri A Jr, Gobbi P, Mazzotti G, Tay FR, Pashley DH, Breschi L: Immunohistochemical and biochemical assay of MMP-3 in human dentine. J Dent 2011;39:231-237.
70.
Mazzoni A, Pashley DH, Nishitani Y, Breschi L, Mannello F, Tjäderhane L, Toledano M, Pashley EL, Tay FR: Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials 2006;27:4470-4476.
71.
Mazzoni A, Pashley DH, Tay FR, Gobbi P, Orsini G, Ruggeri A Jr, Carrilho M, Tjäderhane L, Di Lenarda R, Breschi L: Immunohistochemical identification of MMP-2 and MMP-9 in human dentin: correlative FEI-SEM/TEM analysis. J Biomed Mater Res A 2009;88:697-703.
72.
Mazzoni A, Scaffa P, Carrilho M, Tjäderhane L, Di Lenarda R, Polimeni A, Tezvergil-Mutluay A, Tay FR, Pashley DH, Breschi L: Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. J Dent Res 2013;92:82-86.
73.
Mirigian LS, Makareeva E, Koistinen H, Itkonen O, Sorsa T, Stenman UH, Salo T, Leikin S: Collagen degradation by tumor-associated trypsins. Arch Biochem Biophys 2013;535:111-114.
74.
Mjör IA, Dahl JE, Moorhead JE: Age of restorations at replacement in permanent teeth in general dental practice. Acta Odontol Scand 2000;58:97-101.
75.
Muromachi K, Kamio N, Narita T, Annen-Kamio M, Sugiya H, Matsushima K: MMP-3 provokes CTGF/CCN2 production independently of protease activity and dependently on dynamin-related endocytosis, which contributes to human dental pulp cell migration. J Cell Biochem 2012;113:1348-1358.
76.
Nagase H: Activation mechanisms of matrix metalloproteinases. Biol Chem 1997;378:151-160.
77.
Nakornchai S, Atsawasuwan P, Kitamura E, Surarit R, Yamauchi M: Partial biochemical characterisation of collagen in carious dentin of human primary teeth. Arch Oral Biol 2004;49:267-273.
78.
Nascimento FD, Minciotti CL, Geraldeli S, Carrilho MR, Pashley DH, Tay FR, Nader HB, Salo T, Tjäderhane L, Tersariol IL: Cysteine cathepsins in human carious dentin. J Dent Res 2011;90:506-511.
79.
Nishitani Y, Yoshiyama M, Wadgaonkar B, Breschi L, Mannello F, Mazzoni A, Carvalho RM, Tjäderhane L, Tay FR, Pashley DH: Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. Eur J Oral Sci 2006;114:160-166.
80.
Niu LN, Zhang L, Jiao K, Li F, Ding YX, Wang DY, Wang MQ, Tay FR, Chen JH: Localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 in human coronal dentine. J Dent 2011;39:536-542.
81.
Ohgushi K, Fusayama T: Electron microscopic structure of the two layers of carious dentin. J Dent Res 1975;54:1019-1026.
82.
Okada Y, Naka K, Kawamura K, Matsumoto T, Nakanishi I, Fujimoto N, Sato H, Seiki M: Localization of matrix metalloproteinase 9 (92-kilodalton gelatinase/type IV collagenase = gelatinase B) in osteoclasts: implications for bone resorption. Lab Invest 1995;72:311-322.
83.
Orgel JP, Wess TJ, Miller A: The in situ conformation and axial location of the intermolecular cross-linked non-helical telopeptides of type I collagen. Structure 2000;8:137-142.
84.
Orsini G, Ruggeri A Jr, Mazzoni A, Nato F, Mazzotti G, Putignano A, Tjäderhane L, Breschi L: A review on nature, role and functions of dentin non-collagenous proteins. Part I: proteoglycans and glycoproteins. Endod Topics 2012;21:1-18.
85.
Osorio R, Yamauti M, Osorio E, Ruiz-Requena ME, Pashley DH, Tay FR, Toledano M: Zinc reduces collagen degradation in demineralized human dentin explants. J Dent 2011;39:148-153.
86.
Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S: Collagen degradation by host-derived enzymes during aging. J Dent Res 2004;83:216-221.
87.
Perumal S, Antipova O, Orgel JP: Collagen fibril architecture, domain organization, and triple-helical conformation govern its proteolysis. Proc Natl Acad Sci U S A 2008;105:2824-2829.
88.
Pessoa JI, Guimarães GN, Viola NV, da Silva WJ, de Souza AP, Tjäderhane L, Line SR, Marques MR: In situ study of the gelatinase activity in demineralized dentin from rat molar teeth. Acta Histochem 2013;115:245-251.
89.
Peumans M, Kanumilli P, De Munck J, van Landuyt K, Lambrechts P, van Meerbeek B: Clinical effectiveness of contemporary adhesives: a systematic review of current clinical trials. Dent Mater 2005;21:864-881.
90.
Reis A, Carrilho M, Breschi L, Loguercio AD: Overview of clinical alternatives to minimize the degradation of the resin-dentin bonds. Oper Dent 2013;38:E1-E25.
91.
Saito T, Arsenault AL, Yamauchi M, Kuboki Y, Crenshaw MA: Mineral induction by immobilized phosphoproteins. Bone 1997;21:305-311.
92.
Saito T, Yamauchi M, Abiko Y, Matsuda K, Crenshaw MA: In vitro apatite induction by phosphophoryn immobilized on modified collagen fibrils. J Bone Miner Res 2000;15:1615-1619.
93.
Sano H, Yoshikawa T, Pereira PN, Kanemura N, Morigami M, Tagami J, Pashley DH: Long-term durability of dentin bonds made with a self-etching primer, in vivo. J Dent Res 1999;78:906-911.
94.
Santos J, Carrilho MR, Tervahartiala T, Sorsa T, Breschi L, Mazzoni A, Pashley D, Tay F, Ferraz C, Tjäderhane L: Determination of matrix metalloproteinases in human radicular dentin. J Endod 2009;35:686-689.
95.
Scaffa PM, Vidal CM, Barros N, Gesteira TF, Carmona AK, Breschi L, Pashley DH, Tjäderhane L, Tersariol IL, Nascimento FD, Carrilho MR: Chlorhexidine inhibits the activity of dental cysteine cathepsins. J Dent Res 2012;91:420-425.
96.
Schlueter N, Ganss C, Pötschke S, Klimek J, Hannig C: Enzyme activities in the oral fluids of patients suffering from bulimia: a controlled clinical trial. Caries Res 2012a;46:130-139.
97.
Schlueter N, Glatzki J, Klimek J, Ganss C: Erosive-abrasive tissue loss in dentine under simulated bulimic conditions. Arch Oral Biol 2012b;57:1176-1182.
98.
Schlueter N, Hardt M, Klimek J, Ganss C: Influence of the digestive enzymes trypsin and pepsin in vitro on the progression of erosion in dentine. Arch Oral Biol 2010;55:294-299.
99.
Shimada Y, Ichinose S, Sadr A, Burrow MF, Tagami J: Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine. Aust Dent J 2009;54:347-354.
100.
Sorsa T, Tjäderhane L, Konttinen YT, Lauhio A, Salo T, Lee HM, Golub LM, Brown DL, Mäntylä P: Matrix metalloproteinases: contribution to pathogenesis, diagnosis and treatment of periodontal inflammation. Ann Med 2006;38:306-321.
101.
Stanislawczuk R, Amaral RC, Zander-Grande C, Gagler D, Reis A, Loguercio AD: Chlorhexidine-containing acid conditioner preserves the longevity of resin-dentin bonds. Oper Dent 2009;34:481-490.
102.
Steffensen B, Wallon UM, Overall CM: Extracellular matrix binding properties of recombinant fibronectin type II-like modules of human 72-kDa gelatinase/type IV collagenase. High affinity binding to native type I collagen but not native type IV collagen. J Biol Chem 1995;270:11555-11566.
103.
Sulkala M, Larmas M, Sorsa T, Salo T, Tjäderhane L: The localization of matrix metalloproteinase-20 (MMP-20, enamelysin) in mature human teeth. J Dent Res 2002;81:603-607.
104.
Sulkala M, Tervahartiala T, Sorsa T, Larmas M, Salo T, Tjäderhane L: Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol 2007;52:121-127.
105.
Sulkala M, Wahlgren J, Larmas M, Sorsa T, Teronen M, Salo T, Tjäderhane L: The effect of MMP inhibitors on human salivary MMP activity and caries progression in rats. J Dent Res 2001;80:1545-1549.
106.
Sunnegårdh-Grönberg K, van Dijken JW, Funegård U, Lindberg A, Nilsson M: Selection of dental materials and longevity of replaced restorations in Public Dental Health clinics in northern Sweden. J Dent 2009;37:673-678.
107.
Suppa P, Ruggeri A Jr, Tay FR, Prati C, Biasotto M, Falconi M, Pashley DH, Breschi L: Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. J Dent Res 2006;85:133-137.
108.
Tannure PN, Küchler EC, Falagan-Lotsch P, Amorim LM, Raggio Luiz R, Costa MC, Vieira AR, Granjeiro JM: MMP13 polymorphism decreases risk for dental caries. Caries Res 2012a;46:401-407.
109.
Tannure PN, Küchler EC, Lips A, Costa Mde C, Luiz RR, Granjeiro JM, Vieira AR: Genetic variation in MMP20 contributes to higher caries experience. J Dent 2012b;40:381-386.
110.
Tay FR, Pashley DH: Have dentin adhesives become too hydrophilic? J Can Dent Assoc 2003;69:726-731.
111.
Tersariol IL, Geraldeli S, Minciotti CL, Nascimento FD, Pääkkönen V, Martins MT, Carrilho MR, Pashley DH, Tay FR, Salo T, Tjäderhane L: Cysteine cathepsins in human dentin-pulp complex. J Endod 2010;36:475-481.
112.
Tezvergil-Mutluay A, Agee KA, Hoshika T, Carrilho M, Breschi L, Tjäderhane L, Nishitani Y, Carvalho RM, Looney S, Tay FR, Pashley DH: The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices. Dent Mater 2010;26:1059-1067.
113.
Tezvergil-Mutluay A, Mutluay M, Seseogullari-Dirihan R, Agee KA, Key WO, Scheffel DL, Breschi L, Mazzoni A, Tjäderhane L, Nishitani Y, Tay FR, Pashley DH: Effect of phosphoric acid on the degradation of human dentin matrix. J Dent Res 2013;92:87-91.
114.
Thompson JM, Agee K, Sidow SJ, McNally K, Lindsey K, Borke J, Elsalanty M, Tay FR, Pashley DH: Inhibition of endogenous dentin matrix metalloproteinases by ethylenediaminetetraacetic acid. J Endod 2012;38:62-65.
115.
Tjäderhane L, Carrilho MR, Breschi L, Tay FR, Pashley DH: Dentin basic structure and composition - an overview. Endod Topics 2012;20:3-29.
116.
Tjäderhane L, Haapasalo M: Dentin-pulp border: dynamic interface between hard and soft tissues. Endod Topics 2012;20:52-84.
117.
Tjäderhane L, Hietala EL, Larmas M: Mineral element analyses of carious and intact dentin by electron probe microanalyser combined with back-scattered electron image. J Dent Res 1995;74:1770-1774.
118.
Tjäderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T: The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J Dent Res 1998a;77:1622-1629.
119.
Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol ILS, Geraldeli S, Tezvergil-Mutluay A, Carrilho MR, Carvalho RM, Tay FR, Pashley DH: Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater 2013a;29:116-135.
120.
Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol ILS, Geraldeli S, Tezvergil-Mutluay A, Carrilho MR, Carvalho RM, Tay FR, Pashley DH: Strategies to prevent hydrolytic degradation of the hybrid layer - a review. Dent Mater 2013b;29:999-1011.
121.
Tjäderhane L, Palosaari H, Sulkala M, Wahlgren J, Salo T: The expression of matrix metalloproteinases (MMPs) in human odontoblasts; in Ishikawa T, Takahashi K, Maeda T, Suda H, Shimono M, Inoue T (eds): Proceedings of the International Conference on Dentin/Pulp Complex 2001. Tokyo, Quintessence Publishing, 2002, pp 45-51.
122.
Tjäderhane L, Salo T, Larjava H, Larmas M, Overall CM: A novel organ culture method to study the function of human odontoblasts in vitro: gelatinase expression by odontoblasts is differentially regulated by TGF-beta1. J Dent Res 1998b;77:1486-1496.
123.
Tjäderhane L, Sulkala M, Sorsa T, Teronen O, Larmas M, Salo T: The effect of MMP inhibitor metastat on fissure caries progression in rats. Ann N Y Acad Sci 1999;878:686-688.
124.
Toledano M, Aguilera FS, Yamauti M, Ruiz-Requena ME, Osorio R: In vitro load-induced dentin collagen-stabilization against MMPs degradation. J Mech Behav Biomed Mater 2013;27:10-18.
125.
Toledano M, Nieto-Aguilar R, Osorio R, Campos A, Osorio E, Tay FR, Alaminos M: Differential expression of matrix metalloproteinase-2 in human coronal and radicular sound and carious dentine. J Dent 2010;38:635-640.
126.
Traub W, Jodaikin A, Arad T, Veis A, Sabsay B: Dentin phosphophoryn binding to collagen fibrils. Matrix 1992;12:197-201.
127.
Turk V, Stoka V, Vasiljeva O, Renko M, Sun T, Turk B, Turk D: Cysteine cathepsins: from structure, function and regulation to new frontiers. Biochim Biophys Acta 2012;1824:68-88.
128.
Turk B, Turk D, Turk V: Lysosomal cysteine proteases: more than scavengers. Biochim Biophys Acta 2000;1477:98-111.
129.
Tyas MJ: Placement and replacement of restorations by selected practitioners. Aust Dent J 2005;50:81-89.
130.
Van Nieuwenhuysen JP, D'Hoore W, Carvalho J, Qvist V: Long-term evaluation of extensive restorations in permanent teeth. J Dent 2003;31:395-405.
131.
van Strijp AJ, Jansen DC, DeGroot J, ten Cate JM, Everts V: Host-derived proteinases and degradation of dentine collagen in situ. Caries Res 2003;37:58-65.
132.
Vidal CM, Tjäderhane L, Scaffa PM, Tersariol IL, Pashley D, Nader HB, Nascimento FD, Carrilho MR: Abundance of MMPs and cysteine cathepsins in caries-affected dentin. J Dent Res 2014;93:269-274.
133.
Vitorino R, de Morais Guedes S, Ferreira R, Lobo MJC, Duarte J, Ferrer-Correia AJ, Tomer KB, Domingues PM, Amado FML: Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility. Eur J Oral Sci 2006;114:147-153.
134.
Walton RS, Brand DD, Czernuszka JT: Influence of telopeptides, fibrils and crosslinking on physicochemical properties of type I collagen films. J Mater Sci Mater Med 2010;21:451-461.
135.
Wang Q, Jia P, Cuenco KT, Feingold E, Marazita ML, Wang L, Zhao Z: Multi-dimensional prioritization of dental caries candidate genes and its enriched dense network modules. PLoS One 2013;8:e76666.
136.
Yamakoshi Y, Hu JC, Iwata T, Kobayashi K, Fukae M, Simmer JP: Dentin sialophosphoprotein is processed by MMP-2 and MMP-20 in vitro and in vivo. J Biol Chem 2006;281:38235-38243.
137.
Zehnder M, Wegehaupt FJ, Attin T: A first study on the usefulness of matrix metalloproteinase 9 from dentinal fluid to indicate pulp inflammation. J Endod 2011;37:17-20.
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