The aim of this study was to evaluate the effect of iron II on the dissolution and precipitation of synthetic hydroxyapatite (HA). HA powder was suspended in solutions of iron (0.84 µg/ml, Fe0.84; 18.0 µg/ml, Fe18; 70.0 µg/ml, Fe70), fluoride (1,100 µg/ml, F1,100), and deionized water and submitted to pH cycling. After pH cycling, the samples were analyzed by infrared spectroscopy and X-ray diffraction. The concentrations of fluoride, calcium, phosphorus, and iron were also analyzed. The data were submitted to ANOVA, and analyzed by Tukey’s test (p < 0.05). The infrared spectrum showed a reduction in all bands corresponding to phosphates and hydroxyls and an increase in the carbonate band in the groups with iron. The intensity of the phosphate bands increased and that of the hydroxyl bands decreased in the group F1,100. It was observed that there was a higher concentration of Ca in the group F1,100, with no significant difference between the groups Fe18 and Fe70 (p > 0.05). There was an increase in Fe concentration in the HA directly related to the Fe concentration of the treatment solutions. Results show that the presence of Fe causes the precipitation of apatite with high solubility.

Keywords:
Calcium, Chemical analysis, Fluoride, Hydroxyapatite, Iron
1.
Alves KMRP, Franco KS, Sassaki KT, Buzalaf MAR, Delbem ACB: Effect of iron on enamel demineralization and remineralization in vitro. Arch Oral Biol 2011;58:1192–1198.
2.
Antonakosa A, Liarokapisa E, Leventouri T: Micro-Raman and FTIR studies of synthetic and natural apatites. Biomaterials 2007;28:3043–3054.
3.
Barinov SM, Rau JV, Cesaro SN, Durisin J, Fadeeva IV, Ferro D, Medvecky L, Trionfetti G: Carbonate release from carbonated hydroxyapatite in the wide temperature rage. J Mater Sci Mater Med 2006;17:597–604.
4.
Buzalaf MAR, De Moraes Italiani F, Kato MT, Martinhon CCR, Magalhaes AC: Effect of iron on inhibition of acid demineralisation of bovine dental enamel in vitro. Arch Oral Biol 2006;51:844–848.
5.
Coates J: Interpretation of Infrared Spectra: A Practical Approach; in Meyers RA (ed): Encyclopedia of Analytical Chemistry. Chichester, Wiley, 2000, pp 10815–10837.
6.
Elliott JC: Hydroxyapatite and nonstoichiometric apatites; in Elliott JC: Structure and Chemistry of the Apatites and Other Calcium Orthophosphates. Amsterdam, Elsevier, 1994, pp 111–186.
7.
Fiske CH, Subarrow Y: The colorimetric determination of phosphorus. J Biol Chem 1925;66:375–400.
8.
Gibson IR, Rehman I, Best SM, Bonfiled W: Characterization of the transformation from calcium-deficient apatite to β-tricalciumphosphate. J Mater Sci Mater Med 2000;12:799–804.
9.
Guggenbuhl P, Filmon R, Mabilleau G, Baslé MF, Chappard D: Iron inhibits hydroxyapatite crystal growth in vitro. Metab Clin Exp 2008;57:903–910.
10.
Gutowska I, Machoy Z, Machalinski B: The role of bivalent metals in hydroxyapatite structures as revealed by molecular modeling with the HyperChem software. J Biomed Mater Res 2005;75A:788–793.
11.
Hidaka S, Okamoto Y, Abe K, Miyazaki K: Effects of indium and iron ions on in vitro calcium phosphate precipitation and crystallinity. J Biomed Mater Res 1996;31A:11–18.
12.
Ishikawa K, Ducheyne P, Radin S: Determination of the Ca/P ratio in calcium deficient hydroxyapatite using X-ray diffraction analysis. J Mater Sci Mater Med 1993;4:165–168.
13.
Jha LJ, Best SM, Knowles JC, Rehman I, Santos JD, Bonfield W: Preparation and characterization of fluoride-substituted apatites. J Mater Sci Mater Med 1997;8:185–191.
14.
Joris SJ, Amberg CH: The nature of deficiency in nonstoichiometric hydroxyapatites. II. Spectroscopic studies of calcium and strontium hydroxyapatites. J Phys Chem 1971;75:3172–3178.
15.
Kato MT, de Moraes Italiani F, de Araújo JJ, Garcia MD, de Carvalho Sales-Peres SH, Buzalaf MA: Preventive effect of an iron varnish on bovine enamel erosion in vitro. J Dent 2009;37:233–236.
16.
Kato MT, Maria AG, Sales-Peres SH, Buzalaf MA: Effect of iron on the dissolution of bovine enamel powder in vitro by carbonated beverages. Arch Oral Biol 2007a;52:614–617.
17.
Kato MT, Maria AG, Vaz LG, Italiani FM, Sales-Peres SH, Buzalaf MA: Effect of iron supplementation on the erosive potential of carbonated or decarbonated beverage. J Appl Oral Sci 2007b;15:61–64.
18.
Koumoulidis GC, Katsoulidis AP, Ladavos AK, Pomonis PJ, Trapalis CC, Sdoukos AT, Vaimakis TC: Preparation of hydroxyapatite via microemulsion route. J Colloid Interface Sci 2003;259:254–260.
19.
Kumar GS, Girija EK, Thamizhavel A, Yokogawa Y, Kalkura SN: Synthesis and characterization of bioactive hydroxyapatite-calcite nanocomposite for biomedical applications. J Colloid Interface Sci 2010;349:56–62.
20.
Lynch SR: The impact of iron fortification on nutritional anaemia. Best Pract Res Clin Haematol 2005;18:333–346.
21.
Martinhon CCR, Italiani FM, Padilha PM, Bijella MFTB, Delbem ACB, Buzalaf MAR: Effect of iron on bovine enamel and on the composition of the dental biofilm formed in situ. Arch Oral Biol 2006;51:471–475.
22.
Miguel JC, Bowen WH, Pearson SK: Effects of frequency of exposure to iron sucrose on the incidence of dental caries in desalivated rats. Caries Res 1997a;31:238–243.
23.
Miguel JC, Bowen WH, Pearson SK: Influence of iron alone or with fluoride on caries development in desalivated and intact rats. Caries Res 1997b;31:244–248.
24.
Oppermann RV, Rölla G: Effect of some polyvalent cations on the acidogenicity of dental plaque in vivo. Caries Res 1980;14:422–427.
25.
Pecharki GD, Cury JA, Paes Leme AF, Tabchoury CP, Del Bel Cury AA, Rosalen PL, Bowen WH: Effect of sucrose containing iron (II) on dental biofilm and enamel demineralization in situ. Caries Res 2005;39:123–129.
26.
Qu H, Wei M: Synthesis and characterization of fluorine-containing hydroxyapatite by a pH-cycling method. J Mater Sci Mater Med 2005;16:129–133.
27.
Rintoul L, Wentrup-Byrne E, Suzuki S, Grondahl L: FT-IR spectroscopy of fluorosubstituted hydroxyapatite: strengths and limitations. J Mater Sci Mater Med 2007;18:1701–1709.
28.
Rodríguez-Lorenzo LM, Hart JN, Gross KA: Influence of fluorine in the synthesis of apatites: synthesis of solid solutions of hydroxy-fluorapatite. Biomaterials 2003;24:3777–3785.
29.
Rosalen PL, Pearson SK, Bowen WH: Effects of copper, iron and fluoride cocrystallized with sugar on caries development and acid formation in desalivated rats. Arch Oral Biol 1996;41:1003–1010.
30.
Sales-Peres SHC, Pessan JP, Buzalaf MAR: Effect of an iron mouthrinse on enamel and dentine erosion subjected or not to abrasion: an in situ/ex vivo study. Arch Oral Biol 2007;52:128–132.
31.
Slósarczyk A, Paluszkiewicz C, Gawlicki M, Paszkiewicz Z: The FTIR spectroscopy and QXRD studies of calcium phosphate based materials produced from the powder precursors with different Ca/P ratios. Ceram Int 1997;23:297–304.
32.
Sundaram CS, Viswanathan N, Meenakshi S: Defluoridation chemistry of synthetic hydroxyapatite at nano scale: equilibrium and kinetic studies. J Hazard Mater 2008;30:206–215.
33.
ten Cate JM: Fluorides in caries prevention and control: empiricism or science. Caries Res 2004;38:254–257.
34.
Torell P: Iron and dental caries. Swed Dent J 1988;12:113–124.
35.
Vogel GL, Zhang Z, Carey CM, Ly A, Chow LC, Proskin HM: Composition of plaque and saliva following use of an α-tricalcium-phosphate-containing chewing gum and a subsequent challenge. J Dent Res 2000;79:58–62.
36.
Wilson RM, Elliott JC, Dowker SEP: Formate incorporation in the structure of Ca-deficient apatite: Rietveld structure refinement. J Solid State Chem 2003;174:132–140.
37.
Wilson RM, Elliott JC, Dowker SEP, Rodriguez-Lorenzo LM: Rietveld refinements and spectroscopic studies of the structure of Ca-deficient apatite. Biomaterials 2005;26:1317–1327.
38.
Wopenka B, Pasteris JD: A mineralogical perspective on the apatite in bone. Mater Sci Eng C 2005;25:131–143.
39.
Xu J, Gilson DFR, Butler IS: FT-Raman and high-pressure FT-infrared spectroscopic investigation of monocalcium phosphate monohydrate, Ca(H2PO4)2·H2O. Spectrochim Acta A Mol Biomol Spectrosc 1998;54:1869–1878.
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2012
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