In this study we investigated the effect of fluoride on plaque acid tolerance. The test group consumed 200 ml of milk supplemented with 5 mg F/l as NaF once a day, the milk control group drank 200 ml of unsupplemented milk, and the no-milk control group did not consume milk in this manner. Plaque samples were taken at baseline and after 15 months. The proportion of acid-tolerant bacteria in plaque was estimated using LIVE/DEAD® BacLight™ staining after exposure to pH 3.5 for 2 h. The fluoride group showed a statistically significant decrease in plaque acid tolerance compared to baseline. This study shows that daily intake of fluoride in milk reduces plaque acid tolerance.

Beighton D, Lynch E, Heath MR: A microbiological study of primary root-caries lesions with different treatment needs. J Dent Res 1993;72:623–629.
Beighton D, Russell RR, Whiley RA: A simple biochemical scheme for the differentiation of Streptococcus mutans and Streptococcus sobrinus. Caries Res 1991;25:174–178.
Brailsford SR, Shah B, Simons D, Gilbert S, Clark D, Ines I, Adams SE, Allison C, Beighton D: The predominant aciduric microflora of root-caries lesions. J Dent Res 2001;80:1828–1833.
Hamilton IR: Effects of fluoride on enzymatic regulation of bacterial carbohydrate metabolism. Caries Res 1977;11(suppl 1):262–291.
Hamilton IR, Buckley ND: Adaptation by Streptococcus mutans to acid tolerance. Oral Microbiol Immunol 1991;6:65–71.
Hamilton IR, Svensater G: Acid-regulated proteins induced by Streptococcus mutans and other oral bacteria during acid shock. Oral Microbiol Immunol 1998;13:292–300.
Holdeman LV, Cato EP, Moore WEC (eds): Anaerobe Laboratory Manual, ed 4. Blacksburg, Virginia Polytechnic Institute and State University, 1977.
Kanapka JA, Hamilton IR: Fluoride inhibition of enolase activity in vivo and its relationship to the inhibition of glucose-6-P formation in Streptococcus salivarius. Arch Biochem Biophys 1971;146:167–174.
Lemos JA, Abranches J, Burne RA: Responses of cariogenic streptococci to environmental stresses. Curr Issues Mol Biol 2005;7:95–107.
Mantzourani M, Fenlon M, Beighton D: Association between Bifidobacteriaceae and the clinical severity of root caries lesions. Oral Microbiol Immunol 2009;24:32–37.
Marquis RE: Inhibition of streptococcal adenosine triphosphatase by fluoride. J Dent Res 1977;56:704.
Marsh PD: Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 1994;8:263–271.
Marsh PD: Are dental diseases examples of ecological catastrophes? Microbiology 2003;149:279–294.
McNeill K, Hamilton IR: Effect of acid stress on the physiology of biofilm cells of Streptococcus mutans. Microbiology 2004;150:735–742.
Pratten J, Bedi R, Wilson M: An in vitro study of the effect of fluoridated milk on oral bacterial biofilms. Appl Environ Microbiol 2000;66:1720–1723.
Rundegren J, Hvid EB, Johansson M, Astrom M: Effect of 4 days of mouth rinsing with delmopinol or chlorhexidine on the vitality of plaque bacteria. J Clin Periodontol 1992;19:322–325.
Sutton SV, Bender GR, Marquis RE: Fluoride inhibition of proton-translocating ATPases of oral bacteria. Infect Immun 1987;55:2597–2603.
Svensater G, Borgstrom M, Bowden GH, Edwardsson S: The acid-tolerant microbiota associated with plaque from initial caries and healthy tooth surfaces. Caries Res 2003;37:395–403.
Svensater G, Larsson UB, Greif EC, Cvitkovitch DG, Hamilton IR: Acid tolerance response and survival by oral bacteria. Oral Microbiol Immunol 1997;12:266–273.
Svensater G, Sjogreen B, Hamilton IR: Multiple stress responses in Streptococcus mutans and the induction of general and stress-specific proteins. Microbiology 2000;146:107–117.
Takahashi N, Nyvad B: Caries ecology revisited: Microbial dynamics and the caries process. Caries Res 2008;42:409–418.
van Houte J, Lopman J, Kent R: The predominant cultivable flora of sound and carious human root surfaces. J Dent Res 1994;73:1727–1734.
van Houte J, Lopman J, Kent R: The final pH of bacteria comprising the predominant flora on sound and carious human root and enamel surfaces. J Dent Res 1996;75:1008–1014.
van Houte J, Sansone C, Joshipura K, Kent R: Mutans streptococci and non-mutans streptococci acidogenic at low pH, and in vitro acidogenic potential of dental plaque in two different areas of the human dentition. J Dent Res 1991;70:1503–1507.
Welin-Neilands J, Svensater G: Acid tolerance of biofilm cells of Streptococcus mutans. Appl Environ Microbiol 2007;73:5633–5638.
Welin J, Wilkins JC, Beighton D, Wrzesinski K, Fey SJ, Mose-Larsen P, Hamilton IR, Svensater G: Effect of acid shock on protein expression by biofilm cells of Streptococcus mutans. FEMS Microbiol Lett 2003;227:287–293.
Whiley RA, Beighton D: Current classification of the oral streptococci. Oral Microbiol Immunol 1998;13:195–216.
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