Homogenization of salivary gland tissue from the Macaca irus monkey showed an α-amylase content/g wet weight of parotid tissue about five times that of submandibular tissue. The sublingual gland showed little or no amylase activity. When total size of the glands were considered, 93–95 % of the salivary amylase seemed to be produced in the parotid glands. The results after homogenization of human autopsy material and glandular tissue extirpated after parotid surgery indicated a similar distribution of salivary α-amylase. The Cercopithecus aethiops monkey showed similar concentrations of parotid amylase, but an inverse relationship was found between the content of amylase in the submandibular and the sublingual gland, as compared with that of the Macaca monkey.