Ten healthy adult volunteers were recruited to participate in this double-blind randomised 18-leg crossover designed study. The subjects either rinsed their mouth with 10 ml de-ionised distilled water for 10 s or just spat out once after 1-min brushing with one of nine different toothpastes: NaF (500, 1,000 and 1,450 ppm F), SMFP (525, 1,000, 1,450 ppm F), AmF (250, 1,400 ppm F) or fluoride-free dentifrice. Samples of whole mixed unstimulated saliva were collected at different time intervals. The results showed that the use of the AmF toothpaste (1,400 ppm F) resulted in the highest fluoride content of saliva without water rinsing after 120 min (0.52 ppm F, CI 0.23, 0.81). Two hours after brushing with fluoride toothpaste containing AmF and NaF, the salivary fluoride levels were still higher than baseline levels.

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