Class V cavities were prepared at the cemento-enamel junction on the facial surfaces of 45 extracted human central maxillary incisors. Enamel samples for analysis were obtained by means of perchloric acid etching from circular areas, 1 mm in diameter, positioned 1.5, 3.5, 5.5, and 7.5 mm from the incisal and apical margins of the restorations, respectively. The preparations were filled with Fuji Ionomer Restorative, Type II. The restored teeth were individually suspended in synthetic saliva at 37 °C and the saliva changed at regular intervals. 15 teeth were removed after 1, 3, and 6 months, respectively. Enamel and cementum samples were again obtained from the teeth from sites parallel and adjacent to the previous biopsy sites. The fluoride content of the etching solutions was determined by gas chromatography and the calcium content by atomic absorption spectrophotometry. Fluoride acquired by enamel after 1 month ( ± 2,500 ppm F) was retained after 3 and 6 months. Fluoride acquired by cementum after 1 month ( ± 15,000 ppm F) was significantly reduced after 3 months ( ± 6,000 ppm F) and maintained at this level after 6 months.

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