..., instructions or products referred to in the content or advertisements. A disintegrin and metalloprotease 10 Calcium influx Cyclopiazonic acid Epigallocatechin-3-gallate Thapsigargin Tumor necrosis factor receptor 1 Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that has...

... mitochondria to cytosol and the activation of Caspase 9 and Caspase 12. The downregulation of ERS signaling by CHOP siRNA desensitized esophageal cancer cells to PTE treatment, whereas upregulation of ERS signaling by thapsigargin (THA) had the opposite effect. N-Acetylcysteine (NAC), a ROS scavenger, also...

...: In this study, CD4 + T cells were isolated from the thymus, and the calcium content of CD4 + thymocytes was measured using fura-2 AM and a TILL imaging system. Pyrazole-3 (Pyr3), thapsigargin (TG), and caffeine were used to assess the effects of chloroquine on the intracellular Ca 2+ content of CD4 + T cells...

... cells with ER stress-inducing agents, thapsigargin and/or tunicamycin. Effects of overexpression or siRNA-mediated knockdown of Klotho on UPR signaling was investigated by immunoblotting and Real-time PCR. Results: Elevated Klotho levels in HK-2 cells decreased expression of ER stress markers phospho...

... whether the [Ca 2+ ]i decreases were due to activation of Ca 2+ -ATPase by glucose, we used Ca 2+ -ATPase inhibitors: thapsigargin (TG), cyclopiazonic acid (CPA) and 2,5-di-(tert-butyl)-l,4-benzo-hydroquinone (BHQ). Pretreatment with either TG, CPA, or BHQ abolished the glucose-induced [Ca 2+ ]i and pHj...

...Yeong-Hau H. Lien; Xiaonan Wang; Raul Martínez-Zaguilán Endoplasmic reticulum (ER) Ca 2+ -ATPases regulate intracellular free calcium ([Ca 2+ ] in ) in renal epithelial cells. Interestingly, treatment of certain cell types with ER Ca 2+ -ATPase inhibitors such as thapsigargin (TG), cyclopiazonic...

... Mitochondria Mutants Patch-clamp fura-2 Thapsigargin Cellular P h y sio lo g y and B ioch em istry Cell Physiol Biochem 1997;7:203-218 Christopher M. Fanger Adam Zweifach Ricardo E. Dolmetsch Markus Hoth Richard S. Lewis Department of Molecular and Cellular Physiology. Stanford University School o f...

... on the oscillations. On the other hand, thapsigargin (1 µmol/l), an inhibitor of endoplasmatic reticulum Ca 2++ -ATPase, abolished the oscillations in 7 out of 10 cells. Furthermore, phorbolester TPA (10 µmol/l) acutely inhibited the oscillations. In cells not expressing the ras oncogene but pretreated for 24 h...

...Ana M. Rodríguez-López; Carlos Martínez-Salgado; Nélida Eleno; Miguel Arévalo; José M. López-Novoa The purpose of this study was to examine the mechanisms of thapsigargin-induced apoptosis in rat glomerular mesangial cells and the possible involvement of nitric oxide (NO) in this process...

..., PKA, and PKC and stimulated by PDGF after acute exposition to these agonists. Mesangial cells Intracellular pH Na + /H + exchange Natriuretic peptides Diadenosine polyphosphates Adenosine Platelet-derived growth factor Thapsigargin 2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein...

...′-disulfonic acid (an anionic antiport inhibitor). No effect was observed when the Na + /K + -ATPase or the Na + /Ca 2+ exchanger were inhibited. The suppression of intracellular and extracellular calcium did not induced any change in pHi. The addition of thapsigargin, an activator of capacitative calcium...