...Shenglong Zhu; Ninghan Feng; Guangxiao Lin; Yuelin Tong; Xuan Jiang; Qin Yang; Shunhe Wang; Wei Chen; Zhao He; Yong Q Chen Background/Aims: Rapamycin (Rp), the main mammalian target of rapamycin complex inhibitor, is a promising therapeutic agent for breast cancer. However, metabolic disorders...

...Juan Jin; Kang Hu; Meiyu Ye; Diandian Wu; Qiang He Background/Aims: The purpose of this study was to investigate the impact of rapamycin (RAP) on autophagy in podocytes and the therapeutic effects of RAP on idiopathic membranous nephropathy (IMN). Methods: We established an in vitro model of IMN...

... and cellular autophagy levels. Rapamycin (RAPA) is a new macrolide immunosuppressive agent that is primarily used in oncology, cardiology, and transplantation medicine and has been found to extend the lifespan of genetically heterogeneous mice. Several studies have shown that RAPA may have anti-aging effects...

...Chichi Li; Lechi Ye; Li Yang; Xiaofang Yu; Yucang He; Zhuojie Chen; Liqun Li; Dan Zhang Background/Aims: Ischemia is one of the main causes of the high rate of absorption of transplanted autologous fat. Autophagy allows cells to survive by providing energy under starvation. Rapamycin has been found...

...Yang Bai; Rui Xu; Xueyuan Zhang; Xiaorong Zhang; Xiaohong Hu; Yashu Li; Haisheng Li; Meixi Liu; Zhenggen Huang; Rongshuai Yan; Weifeng He; Gaoxing Luo; Jun Wu Backgroud/Aims: The effects of rapamycin (RPM) on wound healing have been previously studied. However, reciprocal contradictory data have...

...Jie-Ping Wang; Meng-Yu Zhang Background/Aims: Mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase and activation of its signal pathway plays an important role in regulating protein growth and synthesis as well as cell proliferation and survival. In the present study, we...

... agonist (rapamycin, Rap), autophagy inhibitor (3-methyladenine, 3-MA) or PBS for 1 h before H/R treatment for 2 h, 4 h and 6 h. The optimal concentration of Rap (150 nM, 200 nM and 250 nM) or 3-MA (5 mM, 10 mM and 15 mM) was obtained from MTT assay. Autophagy was determined by fluorescence microscopy...

...Xin Tian; Shundong Dai; Jing Sun; Shenyi Jiang; Chengguang Sui; Fandong Meng; Yan Li; Liye Fu; Tao Jiang; Yang Wang; Jia Su; Youhong Jiang Background/Aims: Rapamycin is a potential anti-cancer agent, which modulates the activity of mTOR, a key regulator of cell growth and proliferation. However...

... were subjected to bilateral renal ischemia followed by 0-h to 7-day reperfusion, exposure of TCMK-1 cells to 24-h hypoxia, and 2 to 24-h reoxygenation. The effect of autophagy on apoptosis was assessed in the presence of autophagy inhibitor 3-methyladenine (3-MA) and autophagy activator rapamycin...

...Bin Wang; Wei Ding; Minmin Zhang; Hongmei Li; Yong Gu Background/Aim: Aldosterone (Aldo), a mediator of kidney fibrosis, is implicated in the pathogenesis of chronic kidney diseases (CKD). The aim of this study was to evaluate the regulatory role of rapamycin (Rap) in Aldo-induced...

.... Rapamycin and dibutyryl-cAMP, which inhibited basal signaling via mammalian target of rapamycin (mTOR), restored insulin-induced PKB- but not IRβ phosphorylation. In livers from 7 day bile duct-ligated rats PKB Ser(473) phosphorylation was decreased by about 50%. Conclusion: TLCS induces insulin resistance...