... of the six known MT-MMPs are anchored to the plasma membrane via a GPI anchor, one of which is MT4-MMP. Only little is known about MT4-MMP expression, synthesis, regulation and degradation. Methods: We analyzed several human cancer cell lines as well as tissue homogenates using Western blotting...

...) to mimic the tonicity of the renal medulla environment. Western blot data showed that CFTR and TNR-CFTR total cell protein is increased by hypertonic medium, but using the surface biotinylation technique, only CFTR was found to be increased in cell plasma membrane. Confocal microscopy showed TNR-CFTR...

...Hewan Belete; Lindsay Godin; Randolph Stroetz; Rolf Hubmayr Cell wounding, that is a loss of plasma membrane integrity, is a common everyday occurrence in load bearing organs such as muscle, skin, and bone. In general, these injuries trigger adaptive responses to either restore homeostasis...

... as of integral membrane proteins to the LB. Here we investigated the trafficking of protein and lipid components of plasma membrane between the plasma and limiting LB membrane by over-expressing lysosomal associated membrane protein 3 fused to green fluorescence protein (LAMP-3-GFP) and farnesylated DsRed (DsRed...

... incubated with or without (control) P1 or cholesterol modulators/caveolae disruptors, cholesterol oxidase (CHOX) and methyl-β-cyclodextrin (CD), for 1 h at 37°C. After incubation cells were used for: i) immunoprecipitation, ii) isolation of plasma membrane (PM)-, Golgi complex (GC)-, and non-Golgi complex...

...John T. Penniston; Agnes Enyedi The plasma membrane pump is a primary means by which Ca 2+ is exported from all kinds of mammalian cells. This review focuses on currently active areas of research on this pump, including the topology of the pump, the regulatory domains (including the calmodulin...

... demonstrated a reduced net negative charge of FH-LDL relative to that of NL-LDL. We hypothesize that LDL surface charge may regulate the movement of cholesterol into the SMC plasma membrane where its presence in excess alters SMC function. Taken together, these results implicate a role for FH-LDL cholesterol...

... K+ channels Channel expression Epithelia Plasma membrane Patch clamp Whole-cell clamp MDCK Cell culture Cell Physiol Biochem 1991:1:195-204 © 1991 S. Karger AG. Basel 1015-8987/91/0014-0195S2.75/0 Synthesis of Plasma Membrane and Potassium Channels in Epithelial (MDCK) Cells A. Ponce*, J.J...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 1992 Amphibian urinary bladder Urocytes Plasma membrane Intracellular vacuolation Na+/K+ ATPase Anthroylouabain Original Paper Cell Physiol Biochem 1992;2:253-263...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 1992 Amphibian urinary bladder Urocytes Epithelial cell dissociation Plasma membrane Invagination Vacuolation...

... Transporter structure Transporter expression Plasma membrane Cell Physiol Biochem 1994;4:217-241 Joan Bertrána Xavier Testarb Antonio Zorzanob Manuel Palacínb a Departament de Criobiologia i Terápia Celular, Institut de Recerca Oncológica, Barcelona; b Departament de Bioquímica i Fisiología, Facultat de...

...Sang-Ho Kwon; Harvey Pollard; William B. Guggino ΔF508 CFTR can be functionally restored in the plasma membrane by exposure of the cell to lower temperature. However, restored ΔF508 CFTR has a much shorter half-life than normal. We studied whether NHERF1, which binds to the PDZ motif of CFTR, might...