...Rong Ding; Fei Guo; Yong Zhang; Xi-Mei Liu; Yu-Qian Xiang; Chen Zhang; Zhi-Wei Liu; Jian-Zhong Sheng; He-Feng Huang; Jun-Yu Zhang; Jian-Xia Fan Background/Aims: The placenta has been suggested to play a crucial role in the pathology of gestational diabetes mellitus (GDM). Placenta-specific...

... to identify mRNA and long non-coding RNA (lncRNA) profiles in placenta and gonadal fat of pregnant mice fed a high-fat diet and to investigate the transcripts and pathways involved in the development of gestational diabetes mellitus. Methods: Deep and broad transcriptome profiling was performed to assess...

... of the placenta, although there is limited evidence as to how the ischemia impacts on the placenta. AMP-activated protein kinase (AMPK) is a key regulator of cellular energy metabolism and plays an important role in a variety of ischemic diseases by enhancing energy production. The present study investigated...

... and reduced trophoblast-mediated remodeling of spiral arteries. The aim of this study was to investigate the expression pattern of NTE in the placenta from women with PE and normal pregnancy, and the molecular mechanism of NTE involved in the development of PE. Methods: NTE expression levels in placentas from...

... using TaqMan® Human ABC Transporter Array, in preterm human placentas without (PTD; n=6) or with histological chorioamnionitis (PTDC; n=6). Validation was performed using qPCR, immunohistochemistry and Western blot. MicroRNAs known to regulate P-glycoprotein (P-gp) were examined by qPCR. Results: Up...

...Yanli Gu; Junhui Wan; Lv Yao; Nan-Ni Peng; Wen-Lin Chang Background/Aims: It is well known that Plac1 is a placenta-specific gene; however, its spatiotemporal expression pattern and exact role at t h e mouse fetomaternal interface r e m a i n s unclear. Methods: In situ hybridization (ISH) was used...

... known to elicit adverse fetal neurological outcomes. The P-glycoprotein (P-gp) efflux transporter protects the developing fetus by limiting the transfer of substrates across both the placenta and the fetal blood-brain barrier (BBB). As such, inhibition of P-gp at these blood-barrier sites may result...

... blood pressure (SBP) and urinary protein (UP) were measured. The expressions of JAK2/STAT3/SOSC1 signaling pathway-related proteins in placenta tissues were detect by Western blotting. Radioimmunoassay was applied to detect serum levels of nitric oxide (NO), super oxide dismutase (SOD), placental growth...

...-chain reaction (PCR) was used to determine the expression of TLRs and cytokines in placenta and trophoblasts. The expression of MyD88 was interfered with small interfering RNA (siRNA) in trophoblasts. An in intro model mimicking trophoblast barrier was established to evaluate the effect of MyD88 siRNA...

...Koumei Shirasuna; Narumi Shimamura; Kotomi Seno; Ayaka Ohtsu; Shogo Shiratsuki; Akihide Ohkuchi; Hirotada Suzuki; Shigeki Matsubara; Shiho Nagayama; Hisataka Iwata; Takehito Kuwayama Background/Aims: The placenta is a vital organ for pregnancy. Many in vitro placental experiments are conducted...

...Lan Liu; Xiang Zhang; Can Rong; Can Rui; Hui Ji; Yu-jia Qian; Ruizhe Jia; Lizhou Sun Background: The placenta acts not only as a conduit of nutrient and waste exchange between mother and developing fetus but also functions as a regulator of the intrauterine environment. Pre-eclampsia (PE...

...Zhijing Miao; Min Chen; Hong Wu; Hongjuan Ding; Zhonghua Shi Background: Fetal growth restriction (FGR) is the main cause of intrauterine fetal death and the second leading cause of death in the neonatal period. A large body of evidence suggests that FGR may be associated with the placenta...

...Sulistyo Emantoko Dwi Putra; Corinna Neuber; Christoph Reichetzeder; Berthold Hocher; Burkhard Kleuser Background: DNA-methylation is a common epigenetic tool which plays a crucial role in gene regulation and is essential for cell differentiation and embryonic development. The placenta...

...W. Krick; J. Disser; A. Rabe; E. Frömter; C.P. Hansen; B. Roch; K. Kunzelmann; R. Greger; H.-W. Fehlhaber; G. Burckhardt Cytosol prepared from human placenta inhibits outwardly rectifying Cl - channels excised from HT 29 cells. Size exclusion chromatography of cytosol on Superose 12 revealed two...

...S. Blum; H. Bühler; I. Lichtenstein; H. Siebe Human placenta exhibits high levels of 11β-HSD oxidative activity. The enzymatic activity was tested in tissue slices, homogenates, microsomes and CHAPS-solubilized microsomal protein of spontaneously delivered fresh human placenta. Compared to liver...