... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Potassium channel K v 1.3 channel σ-pore Electrophysiology Patch-clamp Deutsche Foschungsgemeinschaft (DFG) 10.13039/501100001659 K v 1.2 and K v 1.3...

... to the on-rate ( k on ) of verapamil. By comparing on-rates obtained in wild-type (wt) and mutant channels a binding pocket for verapamil and impacts of different amino acid residues should be investigated. Methods: Using the whole-cell patch clamp technique the action of verapamil on currents through wild-type...

... motility, viability and acrosome reaction (AR) ratio were then examined. Furthermore, the current and expression levels of both the sperm-specific Ca 2+ channel (CatSper) and the sperm-specific K + channel (KSper) were evaluated by patch-clamping and western blotting, respectively. Results: Our data showed...

... the cation channels and thus influences eryptosis. Methods: Cation channel activity was determined by whole-cell patch clamp, [Ca 2+ ] i utilizing Fluo3 fluorescence and annexin V binding to estimate cell membrane scrambling with phosphatidylserine exposure. Results: A 45 min exposure to furosemide (10...

... Ion channel regulation Phosphorylation Surface expression Patch-clamp Protein kinase B α Serum- and glucocorticoid-inducible kinase isoform 1 Xenopus laevis oocytes Cellular Physiology and Biochemistry Original Paper Cell Physiol Biochem 2010;26:913-924 Accepted: October 13...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Potassium channel Kidney Patch-clamp Apoptosis Endotoxin Cellular Physiology and Biochemistry Original Paper Cell...

... the whole cell patch-clamp technique, we have recorded prominent inward and outward rectifying voltage-dependent potassium currents but no calcium-activated potassium currents. Using pharmacological, biophysical and molecular approaches, we demonstrate that Kv1.3 and Kir2.1 channels underlie outward...

...) in the membrane of the parasitized erythrocyte which provide the parasite with nutrients, adjust the erythrocyte electrolyte composition to the needs of the parasite, and dispose of metabolic waste products and osmolytes. Patch-clamp recordings identified inwardly and outwardly rectifying (OR) anion conductances...

...–92 were grown on glass coverslips in Dulbecco’s modified Eagle’s medium. Cells were used in a subconfiuent state. Standard patch-clamp experiments were carried out predominantly in the inside/out configuration. Four types of ion channels were found: large-conductance K + channels (bK...

... attractive opportunities for elucidating the molecular basis and consequences of store-operated calcium influx in T cells. Capacitative calcium entry Calcium signaling Calcium oscillations Gene expression Mitochondria Mutants Patch-clamp fura-2 Thapsigargin Cellular P h y sio lo g y and B ioch...

... collecting duct (CCD) segments. [Ca 2+ ] i and membrane voltages (V m ) were measured using the fura-2 method and the slow-whole-cell patch-clamp technique, respectively. Fura-2 fluorescence ratio (FR) at a pH o of 7.4 was 1.04 ± 0.02 (n = 121), corresponding to a calculated [Ca 2+ ] i of 97 ± 6 n M...