... prolongation is lacking. Methods: We tested the affinity of lubeluzole, its enantiomer, and the racemate for hERG channel using the patch-clamp technique. We synthesized and tested two simplified model compounds corresponding to two moieties included in the lubeluzole structure. The obtained experimental...

...). The KCl content of the bath solutions was either 0 or 40 mM. Patch clamp experiments were performed at room temperature in voltage-clamp, fast-whole-cell mode as described previously [ 22 ]. The cells were continuously superfused through a flow system inserted into the dish. The bath was grounded via...

... Potential Duration (APD), sodium current ( I Na ), L-type calcium current ( I Ca, L ) and transient outward potassium current ( I to ) were measured and analysed using a patch-clamp recording technique in normal rat cardiomyocytes and myocytes of arrhythmia aconitine-induced by. Results: Arctigenin...

... to determine the osmotic sensitivity of freshly isolated rat chondrocytes, their RVD capabilities and to study the properties of any anion currents associated. Methods: Cell volume responses were determined by microscopy. Whole cell patch clamp was used to record ion currents. Results: Chondrocytes showed...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Bacteria E. Coli MscL MscS Patch clamp Liposomes EPR spectroscopy FRET...

.... Results: Patch-clamp recordings revealed arrhythmias and delayed afterdepolarizations (DADs) after catecholaminergic stimulation of CPVT1-iPS cell-derived cardiomyocytes. Calcium imaging studies showed that, compared to healthy cardiomyocytes, CPVT1-cardiomyocytes exhibit higher amplitudes and longer...

... the results of the first patch clamp experiments on plant mitochondria. Single-channel recordings in 150 mM potassium gluconate have allowed the biophysical characterization of a channel with a conductance of 150 pS in the inner mitochondrial membrane of mitoplasts obtained from wheat ( Triticum durum Desf...

... responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. HEK293 cell hERG channel Patch clamp Oxymatrine Temperature Cellular Physiology and Biochemistry Original Paper Cell Physiol Biochem 2010;26...

... the poorly metabolizable methylether of apigenin, 5,7,4’-trimethoxyflavone (TMF) as a CFTR activator using transepithelial short-circuit current measurements, whole cell and single cell patch clamp techniques, and nasal potential difference (PD) measurements. Transepithelial Cl - secretion by Calu-3...

... Cystic fibrosis Patch clamp Cl- channels ATP Neurotensin Carbachol Original Paper Cell Physiol Biochem 1993;3:2-16 R. Warth R. Greger Physiologisches Institut der Albert-Ludwigs-Universität. Freiburg, BRD The Ion Conductances of CFPAC-1 Cells Key Words Cystic fibrosis Patch clamp CL channels ATP...

... + currents measured in central neurones. K+ channels Cloned Site-directed mutagenesis Structure-function relation Inactivation Granule cells Glutathione Expression pattern Patch clamp Cell Physiol Biochem 1993;3:250-269 J. Peter Ruppersberg Manfred Ermler Martin Knopf Wilfried Kues Peter Jonas...

... K+ channels Channel expression Epithelia Plasma membrane Patch clamp Whole-cell clamp MDCK Cell culture Cell Physiol Biochem 1991:1:195-204 © 1991 S. Karger AG. Basel 1015-8987/91/0014-0195S2.75/0 Synthesis of Plasma Membrane and Potassium Channels in Epithelial (MDCK) Cells A. Ponce*, J.J...

... cells Whole cell Patch clamp Cl– Channels Cell Physiol Biochem 1991;1:160-169 © 1991 S. KargcrAG, Basel 1015-8987/91/0013-0160S2.75/0 Polarized Distribution of Chloride Channels in Epithelial Cells A. Ponce, R.G. Contreras, M. Cereijido Center for Research and Advanced Studies...

... Ion channels Apical side Basolateral side Polarity Epithelia Madin-Darby canine kidney cells Patch clamp Whole cell clamp Membrane currents Cultured cells Original Papers Cell Physiol Biochem 1991;1:13-23 © 1991 S. Karger AG, Basel IO I5- 8987/ 9 I/OOII- 0 0 13S2.75/0 Polarized...

...Johann S. Schwegler; Stefan Silbernagl The Na + membrane conductance in confluent monolayers of cultured opossum kidney cells has been investigated by the whole-cell patch clamp technique. In symmetrical sodium gluconate solutions Na + conductance rises 10-fold during cytosolic ion equilibration...

... preparation of pancreatic ducts in the electrical parameters, monitored with the whole-cell patch-clamp method, and those in isolated perfused ducts, monitored with the conventional microelectrodes [Novak I, Greger R: Pflügers Arch 1988;411:58-68, 546-553], are comparable. Similar conclusion can be made about...

...Stephan Heinke; Gert Raskin; Patrick De Smet; Guy Droogmans; Willy Van Driessche; Bernd Nilius We have used the whole-cell patch-clamp technique, combined with membrane capacitance (C m ) measurements to study the activation mechanism of swelling-induced Cl – currents in cultured endothelial cells...

... conductances (G c ) and single-channel currents were measured with the patch-clamp method. Cellular Ca 2+ ([Ca 2+ ] i ) and Na + ([Na + ] i ) activities were estimated fluorimetrically with fura-2 and sodium-binding benzofuran isophthalate. Increasing extracellular osmolality by 50 or 100 mosm/1 depolarized V...

...T. Ishikawa; D.I. Cook We have used single-channel patch-clamp techniques to study K + channels in the HSG cell line derived from the ducts of the human submandibular gland. In contrast to the secretory cells of the acini (or endpieces), duct cells are believed to absorb rather than to secrete NaCl...

...Silvia Salemme; Alejandro Rebolledo; Francisco Speroni; Silvana Petruccelli; Verónica Milesi The electrophysiological and pharmacological properties of Ca 2+ current (I Ca ) were determined by the whole-cell configuration of the patch-clamp technique in smooth muscle cells from human umbilical...