... by stimulation of adenylyl cyclase, causing an increase of the intracellular cAMP concentration, activation of the intracellular cAMP effectors protein kinase A (PKA) and Epac, and an efflux of cAMP, the function of which is still unclear. Methods: Activation of adenylyl cyclase by GsPCR agonists or cholera...

... (AT1R) inhibitor Losartan (LST). Moreover, Ang II significantly increased the activities and the expression of proteasome catalytic subunits (β1, β1i, β2i and β5i) in a dose- and time-dependent manner. However, Ang II-induced proteasome activities were remarkably abrogated by LST and PKA inhibitor H-89...

... 4,5-bisphosphate Src PKA hERG PKC I Kr The classical paradigm says that for an arrhythmia to be induced both a substrate and a trigger is needed. The human ether-a-go-go related gene (hERG) encodes the pore forming protein of the rapidly activated delayed rectifier K + current (I Kr...

... explored the detail role of PKA/CREB signaling in BMP9-induced osteogenic differentiation. Methods: Activation status of PKA/CREB signaling is assessed by nonradioactive assay and Western blot. Using PKA inhibitors and a dominant negative protein of CREB (A-CREB), we investigated the effect of PKA/CREB...

... test the effect of amoxapine on rat cerebellar granule neurons (CGNs) to address this possibility. Methods: CGNs cell culture, whole-cell current recording using a patch-clamp technique, western blot and non-radioactive detection analysis of phosphorylated protein kinase A (PKA) were used. Results...

... MAPKs PKA PKC Sugar Cellular Physiology and Biochemistry Original Paper Cell Physiol Biochem 2008;22:715-724 Accepted: September 02, 2008 Lipopolysaccharide Induces Inhibition of Galactose Intestinal Transport in Rabbits in vitro Pilar Amador1, M. Carmen Marca2,5, Josefina García-Herrera1, M...

... production, activation of cAMP-dependent protein kinase A (PKA), and increases in Cl - and Na + transport. Ussing-style electrophysiology showed that the PKA inhibitor, H89, blocked Cl - and Na + transport. Unexpectedly, [Arg 8 ]-vasopressin stimulation resulted in the dephosphorylation of pAMPK thr172 . H89...

...L.C. Schlichter; I. Chung; M.C. Chang Voltage-activated Kv1.3 channels, which are involved in normal functions of human T lymphocytes, contain consensus sites for phosphorylation by PKA, PKC, and CaMKII. Because T-cell functions often depend on second-messenger pathways involving serine/threonine...