... of miR-16-2* to osteoporosis, osteoblast differentiation and mineralization. Methods: The expression pattern of miR-16-2* during osteogenesis or in osteoporosis bone samples was validated by quantitative real-time PCR (qRT-PCR). The human bone marrow mesenchymal stem cells (hBMSCs) were induced...

.... Thus, the objective of the present study was to examine the effects of Ta and Ti surfaces on osteogenesis using rat bone mesenchymal stromal cells (rBMSCs) as a model. Methods: Ta and Ti substrates were polished to a mirror finish to minimize the influences of structural factors, and the intrinsic...

... or shSOST, and then co-cultured with CD4 + T cells isolated from peripheral blood mononuclear cells. The differentiation, adipogenesis, and osteogenesis of Th17 and regulatory T (Treg) cells were examined by western blot, intracellular and intranuclear staining, ELISA, and real-time quantitative PCR...

...-injected with morpholinos and wild-type (WT) MAPK7 messenger RNA (mRNA) at the one-cell stage, followed by calcein staining to evaluate bone formation. hMSCs were transfected with MAPK7 small interfering RNAs and osteogenesis was induced for 14 days. Alizarin red staining was performed and osteoblast...

... the regulation of WNT signalling molecule expression and therefore the osteogenic differentiation of BMSCs during osteogenesis. Methods: Microarrays of BMSCs from elderly individuals or patients with osteoporosis (GSE35959) from the GEO database were analysed using GeneSight-Lite 4.1.6 (BioDiscovery) and C2...

... of hBMSCs isolated from patients with bone nonunion. Highly efficient knockdown of Chordin was achieved in hBMSCs using PSI. Chordin knockdown promoted hBMSC osteogenesis and bone regeneration in vitro and in vivo . Conclusions: Our results suggest that Chordin is a potential target for improving...

..., chondrocyte or myoblast marker expression. More surprisingly, subsequent osteogenesis and/or comparable effects were rapidly induced within 48 h. Conclusion: To the best of our knowledge, this is the first study in which osteogenesis and/or comparable effects were rapidly induced in bone marrow-derived MSCs...

...Yi-Xuan Chen; Dao-Yu Zhu; Zheng-Liang Xu; Jun-Hui Yin; Xiao-Wei Yu; Jiong Mei; You-Shui Gao; Chang-Qing Zhang Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role...

...Guangxin Huang; Yan Kang; Zhiyu Huang; Zhiqi Zhang; Fangang Meng; Weishen Chen; Ming Fu; Weiming Liao; Ziji Zhang Background/Aims: Long noncoding RNAs (lncRNAs) play important roles in stem cell differentiation. However, their role in osteogenesis of human adipose-derived stem cells (ASCs...

... the inhibitory effect of high concentration of TNF-α on osteogenesis. Fig. 4. Increased AdBMP9 titers can partially reverse the suppression of osteogenic marker expression by high concentration of TNF-α (10ng/ml) in iSCAPs. (A) ALP histochemical staining assay. AdBMP9 or AdGFP infection was done...

...Lei Zhang; Guoliang Jiang; Xueling Zhao; Yuekun Gong Background/Aims: We investigated the role of dimethyloxalylglycine (DMOG) in bone marrow mesenchymal stem cell (BMSC) osteogenesis mediated by RhoA/ROCK. Methods: BMSCs were cultured with and without DMOG and/or Y-27632 (ROCK1 inhibitor). Cell...

... to promote osteogenesis and are now used as adjunctive therapy in the clinical setting [ 14,15,16,17,18 ]. However, it is uncertain if BMP2 and BMP7 are in fact the most potent BMPs in inducing bone formation. 3 08 2015 1 9 2015 BMP9 (also known as growth differentiation factor 2, or GDF2...

... effectively promoted osteogenesis and inhibited adipogenesis by simultaneously affecting the Wnt and Hh pathways. Conclusion: These results suggest that LiCl influences the differentiation of BMSCs directly through the Wnt and Hh pathways and thus may be a candidate drug for the treatment of osteoporosis...

...: For huAFMSCs cultured under different conditions, we assayed: expression of the calcitonin receptor, using immunolabelling techniques; proliferation and osteogenesis, using colorimetric and enzymatic assays; intracellular Ca 2+ and cAMP levels, using videomicroscopy and spectrophotometry. Results...

... (BMSCs) away from osteogenesis towards adipogenesis. However, inhibition of TNF-α signaling only yielded moderate inhibition of adipogenesis. Here we showed that in addition to promoting the TNF-α signaling, alcohol also suppressed the Wnt1/β-catenin signaling pathway. Methods : We treated primary BMSCs...

... shifts away from osteogenic to adipogenic lineage. However, how exactly alcohol impairs osteogenesis is still incompletely understood. This study was designed to shed light on this issue. Methods: We treated primary BMSCs from human subjects with alcohol for 24 days. We measured changes of genes related...

... morphogenetic protein 9 (BMP9) is one of the most potent BMPs in regulating osteoblast differentiation of mesenchymal stem cells (MSCs) although the molecular mechanism underlying BMP9-induced osteogenesis remains to be fully elucidated. It is known that epigenetic regulations play an important role...

... in a packaging cell line (EcoPack™2-293) (Clontech, BD, Bioscience, San Jose, California, USA). The control expression vector contains a scrambled (no specific target) shRNA sequence. Proliferation Osteogenesis Sox9 Osteocalcin Adipose-derived stem cell Mesenchymal stem cells (MSC) are able...

... investigated the effects of PNS on gap junction intercellular communication (GJIC) and osteogenesis-associated genes in rat bone marrow stromal cells (BMSCs). Methods and Results:Our MTT assays demonstrated that PNS enhanced BMSC proliferation under basal medium culture in vitro . Alkaline phosphatase (ALP...

.... PD98059 and SB203580, selective inhibitors of ERK1/2 kinase and p38, attenuated the effect of strontium on osteogenesis. Furthermore, it was demonstrated that Rat Sarcoma viral oncogene homolog (RAS), an upstream regulator of ERK1/2 and p38, was activated by strontium treatment and siRNA-mediated Ras...