...Hua Liu; Zhiqing Zhang; Min Xu; Rong Xu; Zhichun Wang; Guangfu Di Background/Aims: New strategies are required to combat neuronal ischemia-reperfusion injuries. K6PC-5 is a novel sphingosine kinase 1 (SphK1) activator whose potential activity in neuronal cells has not yet been tested. Methods...

... staining. The IL-1α and IL-1β levels were correlated with seizure activities. The TNF-α level in the hippocampus was determined at the SRS stage by immunohistochemistry. The effect of GA on ionic currents and action potentials (APs) in NG108-15 differentiated neurons was investigated using patch-clamp...

.... Methods: Mechanically injured spinal cord neurons were incubated with different concentrations of hydrogen in vitro . Immunofluorescence staining and transmission electron microscopy were used to confirm the protective effects of hydrogen. ROS and related proteins were detected with dihydroethidium...

... in the improvement of somatosensory function. Furthermore, compared with NSC and NSC-GFP, NSC-bFGF treatment group appeared to differentiate into more neurons. Conclusion: Taken together, intranasal administration of NSCs is a promising therapy for treatment of neonatal HI, but NSCs overexpressing bFGF promotes...

... is not well understood, although the Sonic hedgehog (Shh) signaling pathway may be involved. Given that resveratrol activates sirtuin (Sirt)1, the present study examined whether this is mediated by Shh signaling. Methods: Primary cortical neuron cultures were pretreated with drugs before oxygen-glucose...

... differentiation towards a neuronal lineage and promoted hNSCs differentiation to glial cells. In contrast, EphB4 overexpression promoted hNSCs self-renewal and proliferation, further induced hNSCs differentiation towards a neuronal lineage and inhibited hNSCs differentiation to glial cells. Moreover, we found...

... laboratory conducted a series of investigations on the neuroprotection of NSP in different cells in the brain. In the present study, we further observe the effects of NSP on neurons and microglia-mediated inflammatory response following oxygen-glucose deprivation (OGD), and explore possible mechanisms...

...Jin Fan; Yuwen Liu; Jian Yin; Qingqing Li; Yiming Li; Jun Gu; Weihua Cai; Guoyong Yin Background/Aims: The purpose of this study was to investigate the role of autophagy in oxygen-glucose-deprivation/reoxygenation (OGD/R) injury in rat neurons . Methods and results: Cortical neurons were isolated...

... cerebellum. Results: Tff3 transcripts were detectable in neural cultures of both the cortex and the hippocampus as well as in glial cell-enriched cultures. Tff3 peptide co-localized with Map2 indicating an expression in neurons in vitro . The neuronal expression was confirmed by immunofluorescence studies...

... hippocampal CA3 neurons, NBCn1 was localized to the postsynaptic membranes of both dendritic shafts and spines and occasionally to the presynaptic membranes. A GST/NBCn1 fusion protein containing the C-terminal 131 amino acids of NBCn1 pulled down PSD-95 from rat brain lysates, whereas GST/NBCn1-ΔETSL...

...John C. Kinnamon The neuron has been studied for over a hundred years, yet our knowledge of the functional morphology of this cell type remains incomplete. There are several features of neurons that are of interest to the structural neurobiologist, including neuronal shape, synapses, gap junctions...

... mechanisms of action underlying the control of the gain of intracellular Ca 2+ release by circulating gonadal steroid hormones. Acute stimulation of isolated neurons with progesterone led to IP 3 R-mediated Ca 2+ transients that depend on the activation of the PI3 kinase/Akt/PKB signaling pathway...