...-time PCR. The levels of MMP-2, MMP-9 and TGF-β1 protein expression were analyzed by zymography and confocal microscopy, respectively. Cell migration was analyzed by wound healing assay. Tumorigenicity of TNBC cells such as tumor growth and metastasis was analyzed using xenograft models. Results...

... viability was measured using the MTT assay, and cell cycle distribution using flow cytometry. A Matrigel-based assay was used to measure in vitro cell migration and invasion. MAPK-related proteins and matrix metalloproteinase (MMP)-9 and MMP-2 expression were measured by western blotting, and MMP2/-9...

.... Previously, Matrix metalloproteinase-9 (MMP-9), monocyte chemotactic protein-1 (MCP-1) and toll-like receptor 4 (TLR4) were confirmed to play an important role in atherosclerosis and plaque instability. Both TLR4 and its negative regulator, Toll-interacting protein (Tollip), could be mediated by EGCG...

...Fatema Al-Rashed; Shihab Kochumon; Safa Usmani; Sardar Sindhu; Rasheed Ahmad Background: Matrix metalloproteinase (MMP)-9 is known to degrade the extracellular matrix and increased MMP-9 levels are related with the pathogenesis of many inflammatory conditions including obesity. Pam3CSK4...

.... The aim of the present study was to evaluate the effects of EGCG on the expression of EMMPRIN and MMP-9 in PMA-induced macrophages, and the potential mechanisms underlying its effects. Methods: Human monocytic THP-1 cells were induced to differentiate into macrophages with phorbol 12-myristate 13-acetate...

...Sardar Sindhu; Areej Al-Roub; Merin Koshy; Reeby Thomas; Rasheed Ahmad Background/Aims: Obese individuals are known to have increased Matrix metalloproteinase (MMP)-9 plasma levels and MMP-9 is reported to play an important role in obesity-associated adipose tissue inflammation. Since in obesity...

.... Metastasis Osteosarcoma microRNA-491-5p rs1056629 MMP-9 We recruited 30 OS patients, who received surgical intervention for the treatment of OS in the First Affiliated Hospital of China Medical University, and obtained biopsy specimens of human OS from primary lesions. Patients who received...

...Yong-Hua Yang; Dong-Ling Li; Xue-Yuan Bi; Lei Sun; Xiao-Jiang Yu; Huan-Le Fang; Yi Miao; Ming Zhao; Xi He; Jin-Jun Liu; Wei-Jin Zang Background: Excessive activation of matrix metalloproteinase 9 (MMP-9) has been found in several inflammatory diseases. Previous studies have shown that acetylcholine...

... and invasive ability of NSCLC cell lines and downregulated the enzyme activity and protein levels of MMP-2 and MMP-9. Conclusions: The expression level of GOLPH3 is correlated with metastasis and prognosis in NSCLC, and GOLPH3 mediates metastasis by regulating the protein levels of MMP-2 and MMP-9 in vitro...

...) containing 10 μM DCF-DA to label intracellular ROS, and then the cells were washed three times with PBS. DCF fluorescence was detected by fluorescence ELISA. HO-1 (heme oxygenase-1) Human lung epithelial cells CD147 MMP-9 High glucose (HG) Reactive oxygen species (ROS) A549 cells were...

...Rasheed Ahmad; Puthiyaveetil Kochumon Shihab; Sara Jasem; Kazem Behbehani Background: Matrix metalloproteinase-9 (MMP-9) is known to be implicated in the pathogenesis of many inflammatory disorders. FSL-1 (fibroblast-stimulating lipopeptide-1) induces cytokine production by monocytes/macrophages...

...Eunsoo Park; Hyereen Kang; Jeonghan Kim; Jesang Ko Background/Aims: Matrix metalloproteinase-9 (MMP-9) plays an important role in tumor invasion and metastasis through the breakdown of extracellular matrix. The c-Jun protein, a major component of the AP-1 transcription factor, is elevated...

...) induce matrix metalloproteinase (MMP)-9 gene expression in macrophages, which has been linked to progress of various inflammatory diseases. The aim of this study was to identify effects of various statins on LPS-induced MMP-9 gene expression in macrophages and microglia. MMP-9 expression was analyzed...

...Hyereen Kang; Sung-Wuk Jang; Jesang Ko Background/Aims: Metastasis is one of the main causes of death for patients with malignant tumors. Induction of matrix metalloproteinase (MMP)-9 is particularly important for the invasiveness of various cancer cells. Celastrol, a triterpenoid isolated from...