... indicated that connexin 43 (Cnx43) is involved in the GJIC response, in the present work we study whether ouabain affects Cnx43 expression and distribution. Methods: We seeded confluent monolayers of epithelial renal MDCK cells and incubated them with 10 nM ouabain during 1 h. Then we measured...

... + extrusion remains unknown. We used Madin-Darby Canine Kidney (MDCK) cells to investigate the effect of acute or chronic high glucose concentration on the abundance and activity of the Na + /H + exchanger (NHE-1). Methods: Using RT-PCR, we also evaluated the mRNA expression for sodium glucose co-transporters...

... localization primarily at the endoplasmic reticulum and plasma membrane. In conclusion, CFTR and TNR-CFTR have different patterns of distribution in MDCK cells and they are modulated by a hypertonic environment, suggesting their physiological importance in renal medulla. 20 07 2010 29 10 2010...

... the PTH-induced increase in [Ca 2+ ] i , when calcium was present in the medium, suggesting that dihydropyridine sensible-type channels are present in the plasma membrane (PM). PTH showed no effect on [Ca 2+ ]i in MDCK cells previously treated with 2 µ M thapsigargine (TP) in [Ca 2+ O] e conditions...

..., and (2) its inhibition with DIDS and DPC does not modify the uptake of [ 14 C]-thymidine. Cl-currents flow through 42 pS channels, located exclusively on the basolateral side. 4 02 1991 19 03 1991 1 07 1991 Transporting epithelia Membrane currents Cell polarity MDCK cells Renal...

..., instructions or products referred to in the content or advertisements. 1991 Aldosterone MDCK cells Na+/H+exchange HCO-3 channels K+ channels H+/K+ pump Cl–7HCO–3 exchange Review Cell Physiol Biochem 1991;1:2-12 © 1991 S. Karger AG. Basel 1015-8987/91/001l-0002$2.75/0 Acute...

...Refugio García-Villegas; Juan Escamilla; Rosana Fiorentino; Marcelino Cereijido The polarized distribution of K + channels in MDCK cells is lost upon harvesting and restored upon re-seeding. Using semi-quantitative PCR, in the present work we find that ( i ) Cells do not “wait” for the normal...