...Zhiqin Li; Jia Wang; Qinglei Zeng; Chunling Hu; Jiajia Zhang; Hongyan Wang; Jingya Yan; Hua Li; Zujiang Yu Background/Aims: HOTTIP is a critical modulator in human diseases including liver cancer, but its role and molecular biological mechanisms in liver fibrosis are still unclear. Methods...

.... The mRNA expression levels of markers of liver fibrosis [alpha-smooth muscle actin (α-SMA) and collagen 1A1 (Col1A1)] were analyzed by quantitative reverse transcription–polymerase chain reaction (RT-PCR). Cell death and proliferation were evaluated by the MTT assay, and biochemical markers of liver damage...

...Lili Zhu; Tingting Ren; Zixin Zhu; Mingliang Cheng; Qiuju Mou; Mao Mu; Yongmei Liu; Yumei Yao; Yiju Cheng; Baofang Zhang; Zhuo Cheng Background/Aims: Hepatic stellate cells (HSCs) are the primary cell type responsible for liver fibrosis. Our study proved that thymosin beta 4 (Tβ4) has anti...

...Liang Li; Wei Wei; Zhenzhen Li; Haiyan Chen; Yu Li; Wei Jiang; Weisan Chen; Guangyao Kong; Jun Yang; Zongfang Li Background/Aims: Liver fibrosis is a complex process of tissue remodeling in response to injury. Hepatic macrophages have been identified as a key player in this process. As the largest...

... in BDL-induced rodent cholestatic liver fibrosis. In addition, we showed that TC, the main component of BAs, enhanced the synthesis of CTGF/ CCN2 in AML12 and BRL hepatic cell lines. Moreover, we demonstrated that TC activated ERK, Akt, and YAP signaling in hepatocytes, but the precise roles...

...Naglaa K. Idriss; Hayam G. Sayyed; Amany Osama; Dina Sabry Background/Aims: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated the therapeutic efficacy of intravenous...

... induced by high fat diet (HFD). Here, we examined the effects of FNDC5 on liver fibrosis and underline mechanisms. Methods: Experiments were carried out on wild-type and FNDC5 -/- mice, primary mouse hepatic stellate cells (HSCs) and human hepatic stellate cell line (LX-2). The mice were fed with HFD...

... of PRP, we designed this study to explore the hypothesis that PRP could play a role in improving the biochemical and molecular changes that occur in liver fibrosis induced by dimethylnitrosamine (DMN) in rats. Methods: Four groups were studied: control, PRP control, DMN (liver fibrosis), and DMN+PRP...

... rats were sacrificed at 4, 6, 8, or 10 weeks, respectively, and the degree of liver fibrosis was evaluated by microscopy. Cell purity was verified by immunohistochemical examination using monoclonal antibodies specific for desmin (1:100; Sigma-Aldrich, St. Louis, MO, USA). Immunohistochemical...

...Frdoos Al Fadel; Susann Fayyaz; Lukasz Japtok; Burkhard Kleuser Background/Aims: Ectopic lipid accumulation in hepatocytes has been identified as a risk factor for the progression of liver fibrosis and is strongly associated with obesity. In particular, the saturated fatty acid palmitate...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Hedgehog Radiation-induced liver disease Fractionated irradiation Liver fibrosis Radiation therapy is one of the most effective non-surgical treatments for many...

... that miR-125a-5p is correlated with hepatitis B virus replication and disease progression. However, little is known about the biological role and underlying mechanism of miR-125a-5p in liver fibrosis. Methods: We analyzed the level of miR-125a-5p in carbon tetrachloride-induced liver fibrosis and activated...

... ischemia/reperfusion injury, although little is known about its role in hepatic fibrosis. Methods: Here, by using genetically deficient mice, we reported that PTPRO knockout (PTPRO -/- ) significantly attenuated liver injury, release of inflammatory factors, tissue remodeling, and liver fibrosis in two...

... was significantly reduced during the progression of liver fibrosis. The depletion of Tβ4 significantly promoted the proliferation and migration of LX-2 cells via the activation of the PI3K/Akt signaling pathway. The pro-migratory and pro-proliferative effects of Tβ4 depletion in LX-2 cells can be counteracted...