... and organs, including the kidney. However, the underlying molecular mechanisms of fluoride-induced renal cell-cycle changes are not well understood at present. In this study, we used a mouse model to investigate how sodium fluoride (NaF) induces cell-cycle changes in renal cells. Methods: Two hundred forty...

...Yan Gao; Chen Liang; Jianhai Zhang; Junjie Ma; Jinming Wang; Ruiyan Niu; Chiranjeevi Tikka; Yewei Wang; Jundong Wang Background/Aims: We investigated the combined toxic effect of sodium fluoride (NaF) and sulfur dioxide (SO 2 ) on kidney morphological changes and DNA damage in male Wistar rats...

...-being and by bodybuilders for muscle growth. However, the use of ecdysone in mammals is also associated with kidney growth and enlargement, indications of disturbed kidney homeostasis. The underlying pathogenic mechanism remains to be clarified. Methods: Virtual screening tools were employed to identify...

... of intrarenal VX2 tumors. Furthermore, perfusion CT of the kidney was performed in each group. Prior to perfusion CT scan in group C, the mean arterial blood was elevated to 150-160 mmHg by angiotensin II. The BF, blood volume (BV), mean transit time (MTT), capillary permeability-surface area product (PS...

...: We cultured normal rat kidney epithelial (NRK-52E) cells, human kidney epithelial (HK-2) cells and human renal cell adenocarcinoma (769-P) cells simultaneously under normoxia and hypoxia (1% O2) for 24-72 hours. The protein levels of P-ERK1/2, ERK1/2, P-p38, p38 and eEF2K were detected by western...

... effect of RSV in inhibiting inflammatory responses, reducing oxidative stress, and decreasing cell apoptosis in vivo and in vitro . Methods: RSV was administered before renal ischemia and H 2 O 2 induction. Serum and kidneys were harvested 24 h after reperfusion and NRK-52E cells were collected 4 h after...

...Xiaomei Liu; Jun Wang; Linlin Gao; Hao Liu; Caixia Liu Background: It is well recognized that adverse events in utero can impair fetal development and lead to the development of kidney injury and hypertension in adulthood. We previously reported a lower kidney index, glomeruli number, and decreased...

.... Results: Genetic knockout of SGLT1 ( Slc5a1 –/– mice) significantly compromised bacterial clearance following Listeria monocytogenes infection with significantly enhanced bacterial load in liver, spleen, kidney and lung, and significantly augmented hepatic expression of TNF-α and IL-12a. While all wild...

...Liudmila Cebotaru; Valeriu Cebotaru; Hua Wang; Lois J. Arend; William B. Guggino Background/AIM: STIM1 is as an essential component in store operated Ca 2+ entry. However give the paucity of information on the role of STIM1 in kidney, the aim was to study the function of STIM1 in the medulla...

.../daily); group 3: treated with oral honey (1g /kg.b.wt/daily) and oral lead (2 g/kg.b.wt/daily), and group 4: received oral honey (1 g/kg.b.wt/daily). Honey and lead were given daily during 24 days of experimentation. Laboratory tests and histopathological evaluations of kidneys were done. Results: Oral...

... and/or the nature of the model system used in the study. Methods: Pendrin activity was evaluated by measuring pendrin-dependent iodide influx following overexpression of the transporter in a human kidney cell line, in the presence of selected test compounds or the respective vehicles. Results: Pendrin activity...

...Carolina Monteiro de Lemos Barbosa; Jackson Souza-Menezes; Andressa Godoy Amaral; Luiz Fernando Onuchic; Liudmila Cebotaru; William B. Guggino; Marcelo M. Morales Background: Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the development of multiple, progressive, fluid...

... collected from the tail vein to measure metabolic parameters and renal function. To remove the remaining blood, the kidney tissues were perfused with ice-cold, isotonic phosphate-buffered saline (PBS; pH 7.4). A portion of the kidney tissue was immediately fixed in 10% formalin overnight for histological...

... principal cells of kidney collecting duct fragments underwent hypo-osmotic challenge applied apically or basolaterally and the regulatory volume decrease (RVD) was measured by the calcein quenching method. The AQP -2, -3 and -4 content of the plasma membrane fraction was quantified by Western blotting...

...Nicole B. Kampik; Nicole Gehring; Udo Schnitzbauer; J. Christopher Hennings; Christian A. Hübner; Carsten A. Wagner Background: The Slc4 family of bicarbonate transporters consists of several members, many of which are highly expressed in the kidney and play an important role in acid-base...

... expression patterns were identified through competitive RT-PCR and Western analysis. Stress responses were assayed in thapsigargin-treated proximal tubule cells and in ischemic rat kidney. Results: A previously described NF-κB site and a newly identified Sp/KLF factor binding site were shown to be important...

... not show any significant statistical difference when compared to control mice. Similarly, blood, kidney or liver function tests showed no toxicity with Pistacia lentiscus oil when compared to the control group. Examination of gastrointestinal tissues sections revealed similar structural features...

...Cuifen Wang; Miaozong Wu; Ravikumar Arvapalli; Xiaoniu Dai; Muhammad Mahmood; Henry Driscoll; Kevin M. Rice; Eric Blough Background/Aims: Obesity is an independent risk factor for the development of kidney disease. The purpose of this study was to determine how obesity may contribute to renal...

..., instructions or products referred to in the content or advertisements. Epithelial cells Kidney GTP AQP Water channel Cell cycle M1-CCD cells The P2 purinoceptors are a family of ATP-gated receptors that are expressed on epithelial cells of the kidney, in the apical and basolateral membranes...

..., cardiomyopathy, nephropathy, and urinary infections, the kidney and pancreas are the potential target organs affected in the above conditions. However, the precise molecular mechanisms of disease progression and complications are still unclear. The Zucker homozygous (FA/FA) diabetic fatty (ZDF) rat is a genetic...