... glucose metabolism. [email protected] 28 05 2017 5 12 2017 15 2 2018 Fgf-21 Hepatocytes Glucose metabolism GLUT1 © 2018 The Author(s). Published by S. Karger AG, Basel 2018 Open Access License / Drug Dosage / Disclaimer This article is licensed under...

...Frdoos Al Fadel; Susann Fayyaz; Lukasz Japtok; Burkhard Kleuser Background/Aims: Ectopic lipid accumulation in hepatocytes has been identified as a risk factor for the progression of liver fibrosis and is strongly associated with obesity. In particular, the saturated fatty acid palmitate...

...Ying Hu; Jihua Xue; Ying Yang; Xiaotang Zhou; Chaochao Qin; Min Zheng; Haihong Zhu; Yanning Liu; Weixia Liu; Guohua Lou; Jing Wang; Shanshan Wu; Zhi Chen; Feng Chen Background: Lipocalin 2 (LCN2), a protein primarily produced by hepatocytes, is highly upregulated under various conditions...

..., 12.5, 14.5, 16.5, and 18.5). The liver weight was used to evaluate the liver growth during pregnancy. Hepatocytes proliferation was determined by albumin and 5-ethynyl-2'- deoxyuridine (EdU) double immunostaining while TCs were counted by double immunolabeling for CD34/PDGFR-α. Results: Pregnancy...

... of human livers and primary human hepatocytes. Hepatocytes were treated with PF-429242 to inhibit the proteolytic activation of SREBP precursor protein. SREBP1a-specifc and pan-SREBP1 knock-down were performed by transfection of respective siRNAs. Lipogenic SREBP-target gene expression was analyzed by real...

... signaling in different cell types. In this review, we summarize the current state of knowledge about S1P and insulin signaling in insulin sensitive cells. A specific focus is put on the action of S1P on hepatocytes, pancreatic β-cells and skeletal muscle cells. In particular, modulation of S1P-signaling can...

..., and in turn, protects against mitochondria-dependent cell death. The present study was designed to explore the effects of autophagy on the Cr(VI)-induced hepatotoxicity. Methods: L-02 hepatocytes were incubated with different concentrations of Cr(VI) for 24h and several indicators for evaluating mitochondrial...

... acid metabolism in the liver, especially in dairy cows that experience negative energy balance (NEB) during early lactation. Methods: In this study, using fluorescent quantitative RT-PCR, ELISA, and primary hepatocytes cultured in vitro , we examined the effect of NEFA (0, 0.2, 0.4, 0.8, 1.6, and 3.2...

... not been determined. Methods: To compare the effects of increased FFAs and glucose levels on the PARP-NAD-SIRT1 pathway, which modulates insulin sensitivity, we cultured HepG2 hepatocytes with 300 or 500 µM oleic acid (OA) or 30 mM glucose for 1-4 days. PARP activity, NAD level, SIRT1 expression...

...Jingjing Zhao; Juntian Liu; Xiaoming Pang; Shuyue Wang; Di Wu; Xiaolu Zhang; Liuxin Feng Background: C-reactive protein (CRP) participates in development of inflammatory diseases. Hepatocytes are a major contributor of circulating CRP. Although angiotensin II (Ang II) is known to evoke inflammatory...

...-coupled receptor (GPCR) agonists in cultured rat hepatocytes. The aim of the present study was to examine the mechanisms involved in induction of LRF-1/ATF3 by the GPCR agonist vasopressin. Methods: Primary cultures of rat hepatocytes were treated with vasopressin, TPA, and the Ca 2+ -elevating agents...

...Ralf Kubitz; Rainer Schreiber; Stephan vom Dahl; Dieter Häussinger In rat hepatocytes loaded with fluorescein isothiocyanate (FΙTC)-dextran, ethanol (100 m M) led to a biphasic increase of the pH of endocytotic vesicles (control pH ves = 6.11 ± 0.06, n = 77) starting with a peak (ΔpH ves = +0.15...

...Susanna Iossa; Giovanna Liverini; Antonio Barletta The study shows that in rats acclimated to 4°C for 15 days the following parameters related to gram wet liver significantly changed in respect to control rats (24°C). The respiration rates of isolated hepatocytes without added substrates...

..., with a calculated molecular mass of 39 kD. It is predicted to span the membrane 7 times and is only expressed in differentiated hepatocytes of mammalian liver. Functionally, it mediates selective Na + -dependent bile acid uptake, oatp is a glycoprotein of 670 amino acids with a calculated molecular mass of 74 kD...

...H. Apfel; C.A. Wagner; M. Schmitt; S. Waldegger; D. Häussinger; F. Lang As evident from conventional electrophysiology, serotonin leads to a rapid and fully reversible depolarization of the rat hepatocyte cell membrane. At 10 µmol/l serotonin, the depolarization approached 7.3 ± 0.6 mV...

... in the treatment of type 2 diabetes, owing to its anti-hyperglycemic properties. However, the mechanism(s) underlying these metabolic effects remains unknown. Methods: The effects of silibinin on liver gluconeogenesis were studied by titrating hepatocytes from starved rats with sub-saturating concentrations...

...S. Breit; H.-A. Kolb; D. Häussinger; F. Lang As shown previously, tetrabutylhydroperoxide (t-BOOH) shrinks hepatocytes by release of cellular K + . The hyperpolarization, the increase of K + selectivity, and the Ba 2+ sensitivity of K + release have been taken as evidence of activation of K...

.... Anisosmolarity Cell volume CD9 antigen Gene expression Hepatocytes Kupffer cells Original Paper Cell Physiol Biochem 1999;9:99 105 Accepted: March 1, 1999 Lack of Osmosensitive CD9 Expression in Rat Liver Cells Ulrich Warskulat Rainer Hammermann Dieter Häussinger Medizinische Universitätsklinik der...