... was transduced into hPMSCs using a lentivirus to establish GFP + hPMSCs. GFP + hPMSCs were analyzed for their phenotypic profile, viability and adipogenic, osteogenic and hepatic differentiation. The derived GFP + hepatocyte-like cells were evaluated for their metabolic, synthetic and secretory functions...

...) to study the effects of SREBP-1c on lipid deposits in bovine hepatocytes. The expression levels and enzyme activities of SERBP-1c and its target genes were determined by real-time PCR, western blot, and ELISA. Results: These results showed that Ad-GFP-SREBP-1c could inhibit SREBP-1c expression...

...) is predominantly expressed in the liver and at lower levels in the kidneys and at the brain surface. Am J Physiol - Renal Physiol 2012;302:F316-F328. 34. Sacci JB: Hepatocyte perfusion, isolation and culture. Methods Mol Med 2002;72:503-505. 35. Kempson SA, Parikh V, Xi L, Chu S, Montrose M: Subcellular...

..., USA) with bovine serum albumin used as concentration standards. Standards and the samples were measured in duplicates and the mean values were used to calculate the protein concentrations. Mitochondria Hepatocyte Electron leak GSH Hexavalent chromium (Cr(VI)) is a well-known highly...

...Xiao-Geng Deng; Rong-Lin Qiu; Zhi-Xi Li; Jie Zhang; Jia-Jia Zhou; Yao-Hao Wu; Le-Xiang Zeng; Jing Tang Backgroud/Aim: Because of the oncogenic risk, it is important to gain the homogeneous and purified cells from differentiated ESCs before transplantation. Here, we aim to select hepatocyte-like...

...Su-Mei Tsai; Wen-Pin Wang Background/Aim: Previous studies have shown that fibroblast growth factors (FGFs) are involved in the process of liver injury repair. Liver regeneration after partial hepatectomy (PH) is impaired in transgenic mice expressing dominant-negative FGFR2b in hepatocytes...