... be involved in σ-pore formation. Results: Overexpression of the trimeric h K v 1.3_V388C channel in COS-7 cells yielded typical σ-pore currents at potentials more negative than -100 mV similar to what was observed for the tetrameric h K v 1.3_V388C channel. Electrophysiological properties of the trimeric...

..., GFP (eGFP-N1 plasmid DNA; Clontech Laboratories, Inc., Palo Alto, CA, USA). The transfected cells were used for electrophysiological measurements 1-3 days after transfection when sufficient protein was expressed at that time. Current decay time constants (τ decay ) were obtained by fitting...

.... CFTR mutants Cystic fibrosis Electrophysiology Heat shock protein Intracellular trafficking Membrane-spanning Precision medicine Protein folding Proteostasis network National Institutes of Health (NIH) 10.13039/100000002 Cellular Physiology Cell Physiol Biochem 2017;41:2194-2210...

... as electrophysiological alterations, which are related to neurodevelopmental disorders and neurodegenerative diseases. The toxic effects of inorganic nanomaterials on the cytoskeleton and electrophysiology are summarized in this review. The relationships between inorganic NPs-induced cytoskeletal and electrophysiological...

...) requires comparability of cardiac differentiation between different ESC lines. To investigate whether the outcome of cardiac differentiation is consistent between different ESC lines, we compared electrophysiological properties of ESC-derived cardiomyocytes (ESC-CMs) of different murine ESC lines. Methods...

... Endophilin A3 Protein-protein-interaction Yeast two-hybrid experiments Pull-down assays Electrophysiology Ca 2+ Measurements Cells were cultured with MEM medium containing glutamax-I and Earle`s salts (Invitrogen, Karlsruhe, Germany) and 10 % HS/5 % FCS for rat pheochromocytoma PC12 cells...

... systems to model organisms. To date, there is no model available which is low-cost and accurately reflects the complex electrophysiology of the human heart. The average cost of developing a new medication is approximately 802 Million US$ and takes 12 years [ 1 ] at which point the preclinical...

.... Osmolyte transporter Neurodegeneration Kinase Electrophysiology At least in theory, TTBK2 sensitive BGT1 activity could modify neuronal function and survival in addition by transport of GABA. BGT1 mediates the cellular uptake of GABA, a function which was expected to decrease extracellular GABA...

... Sanderson and Augustus Waller. In 1903 Willem Einthoven used the much more sensitive string galvanometer and was awarded Nobel Price in Medicine for this discovery. Though the physical principles of that era are still in use, there have been many advances but also challenges in cardiac electrophysiology...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 5-BDBD P2X receptors Calcium infux Electrophysiology ATP Extracellular ATP and its breakdown products...

..., instructions or products referred to in the content or advertisements. K + channel Cardiac repolarization Tumor cells PKB AKT PIKfyve Electrophysiology The human ether-a-go-go (hERG) channels contribute to cardiac repolarization [ 1,2 ]. Moreover, hERG channels are expressed in several...

... with NBCn1 and increases its channel-like activity while negligibly affecting Na/HCO 3 cotransport. The possibility that the channel-like activity occurs via an intermolecular cavity of multimeric NBCn1 proteins is discussed. Bicarbonate transport Acid-base pH Electrophysiology Neuron Xenopus...

... of this study was to set up and validate an in vitro experimental system for studies on the effects of LPC on electrophysiological parameters in beating cardiomyocytes. Methods and Results: Spontaneously beating HL-1 cardiomyocytes were cultured on multielectrode array microchips for three days...

... Kinase Electrophysiology &HOOXODU 3K\VLRORJ\ DQG %LRFKHPLVWU\ &HOO 3K\VLRO %LRFKHP '2 3XEOLVKHG RQOLQHXO 6DUJHUDVHO ZZZ NDUJHU FRP FSE $$OFHFVHXSWWDHQG 6 -RXSQMHD Q Ls UP DNX 6RSMDQL 0XQR] 9RHONO /D Q J7 76 H Q VL W LY LWR I 67 2ULJLQDO 3DSHU 8SUHJXODWLRQ RI 1D FRXSOHG *OXFRVH WUDQVSRUWHU...

... channel Sodium transport Xenopus laevis oocytes Electrophysiology NMR Peptides Cellular Physiology and Biochemistry Original Paper Cell Physiol Biochem 2012;29:761-774 Accepted: February 23, 2012 An Inhibitory Peptide Derived from the -subunit of the Epithelial Sodium Channel (ENaC) Shows...

... nanoparticles, fullerenes, single- and multi-wall carbon nanotubes (SWCNT, MWCNT) were used in these experiments. Electrophysiological measurements were performed to assay transepithelial electrical resistance (TEER) and epinephrine-stimulated chloride (Cl - ) ion secretion of epithelial cell monolayers...

... characteristics. Recently, scientists found that the electrophysiology of fish cardiomyocytes largely resembles that of humans. So far, cardiomyocyte models were generated using differentiation medium, were stimulated electrically or, when contracting spontaneously, only did so over a short time period. We...

...Chuanchau J. Jou; Kenneth W. Spitzer; Martin Tristani-Firouzi Background/Aims: The zebrafish is an emerging model system for the study of cardiac electrophysiology and human arrhythmias. High resolution imaging techniques are powerful tools for the study of zebrafish cardiac electrophysiology...

.... The antiarrhythmic potential and electrophysiological effects of F 2 on rat cardiac tissues were investigated. Methods and Results: In Langendorff-perfused rat hearts, the ventricular arrhythmias were induced by left anterior descending coronary artery of rat heart ligated for 20 min before the release...

... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Xenopus laevis oocytes Electrophysiology Heterologous expression Ion...