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Keywords: DNA-free
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Cellular Physiology and Biochemistry (2015) 35 (4): 1360–1371.
Published Online: 12 February 2015
... and DNA-free delivery methods for various cell types are required. Methods: Relying on an initially established PCR-based protocol for convenient template DNA production, we synthesized five differently modified EGFP mRNA (mmRNA) species, incorporating various degrees of 5-methylcytidine-5'-triphosphate...