... antimicrobial peptide hBD-2 with APKAM to produce A-hBD-2 and analyzed the effect on wound healing both in vitro and in vivo . Methods: The effects of A-hBD-2 and hBD-2 on cytotoxicity and proliferation in keratinocytes were assessed by Cell Counting Kit-8 assay. The structural stability and antimicrobial...

.... However, in non-tumor cell lines, the respective IC 50 values of JCo extract were 76–105 µg/mL and 77–108 µg/mL. The JCo extract had a stronger cytotoxicity and a larger range of IC 50 values in glioma than in normal cells as compared to those effects caused by temozolomide (TMZ). In addition, the results...

... of Abp1. Abp1 was co-cultured with A. baumannii (AB1) over a range of multiplicities of infection to determine its bactericidal efficacy. HeLa or THP-1 cells were used in the cytotoxicity and protection assays. Finally, the therapeutic effects of Abp1 on local and systemic A. baumannii infection in mice...

...Katharina Helm; Marlena Beyreis; Christian Mayr; Markus Ritter; Martin Jakab; Tobias Kiesslich; Kristjan Plaetzer Background/Aims: For in vitro cytotoxicity testing, discrimination of apoptosis and necrosis represents valuable information. Viability analysis performed at two different time points...

...: In the present study, we exposed a mouse embryonic fibroblast cell line (NIH3T3) to Cd for 24 h, followed by a 24 h BPA exposure to evaluate toxicity. The cytotoxicity was evaluated by viability with CCK-8 assay and lactate dehydrogenase (LDH) release. Reactive oxygen species (ROS) production was measured by 2...

... mechanisms of Ngb action are largely unknown. Ras homolog (Rho) guanosine triphosphatases (Rho GTPases) are involved in the regulation of a number of cellular processes, including cell cytotoxicity. It has been reported that Ngb can act as a guanine nucleotide dissociation inhibitior (GDI) role to inactivate...

... cells. Conclusion : Our study indicates that CD8 + T cells mediate cytotoxicity toward Schwann cells and play an important role in the development of DPN. © 2013 S. Karger AG, Basel 2013 Open Access License / Drug Dosage / Disclaimer Open Access License: This is an Open Access article licensed...

... T0901317 also affected SIRT1 expression. Conclusion: FFAs modulated cellular function through multiple ways, and induced more rapid and more potent cytotoxicity than glucose. 20 08 2013 13 9 2013 © 2013 S. Karger AG, Basel 2013 Open Access License / Drug Dosage / Disclaimer Open Access...

... in vivo , including the central nervous system and exhibits high neurotoxicity in vitro . Methods: We used pharmacological tools to inhibit the cytotoxic effect of the toxin in primary cultured neurons. Immunocytochemical techniques in combination with confocal microscopy were employed to examine...