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1-20 of 124
Keywords: Cell volume
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Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2018) 51 (2): 501–512.
Published Online: 20 November 2018
... was estimated using annexin-V-binding, cell volume using forward scatter, [Ca 2+ ] i using Fluo3-fuorescence, reactive oxygen species (ROS) formation using 2’,7’-dichlorodihydrofuorescein (DCF)-dependent fluorescence, and ceramide abundance using specific antibodies. Results: A 48 hours exposure of human...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2018) 50 (6): 2283–2295.
Published Online: 13 November 2018
...: Phosphatidylserine exposure at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, reactive oxygen species (ROS) formation from 2’,7’-dichlorodihydrofluorescein (DCF)-dependent fluorescence, and ceramide abundance utilizing specific antibodies...
Journal Articles
Subject Area:
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Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2018) 45 (4): 1707–1716.
Published Online: 23 February 2018
... slightly, but significantly decreased [Ca 2+ ] i without significantly modifying the other measured parameters. CRP significantly increased [Ca 2+ ] i , ROS abundance, P-selectin abundance, activated α IIb β 3 integrin, annexin-V-binding, caspase activity as well as aggregation and decreased cell volume...
Journal Articles
Subject Area:
Further Areas
Martin Jakab, Nina Ketterl, Johannes Fürst, Marlena Beyreis, Michael Kittl, Tobias Kiesslich, Cornelia Hauser-Kronberger, Martin Gaisberger, Markus Ritter
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 43 (3): 1037–1051.
Published Online: 02 October 2017
...) and cytotoxicity assays. The mean cell volume (MCV), cell granularity (side-scatter; SSC), phosphatidylserine (PS) exposure, cell membrane integrity, caspase activity and the mitochondrial membrane potential (ΔΨ m ) were measured by flow cytometry. Results: We found that the α-subunit of the non-gastric H + /K...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 43 (2): 431–444.
Published Online: 01 September 2017
... of phosphatidylserine exposure at the cell surface with annexin-V, cell volume with forward scatter, cytosolic Ca 2+ activity ([Ca 2+ ] i ) with Fluo3, oxidative stress with 2′,7′-dichlorodihydrofuorescein diacetate (DCFDA), glutathione (GSH) with mercury orange 1(4-chloromercuryphenyl-azo-2-naphthol), lipid...
Journal Articles
Subject Area:
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Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 41 (6): 2279–2288.
Published Online: 26 April 2017
... were exposed to medium without or with 500 µg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential...
Journal Articles
Subject Area:
Further Areas
Majed Abed, Christian Thiel, Syeda T. Towhid, Kousi Alzoubi, Sabina Honisch, Florian Lang, Alfred Königsrainer
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 41 (2): 806–818.
Published Online: 14 February 2017
... from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, ceramide abundance and caspase-3-activity utilizing FITC-conjugated antibodies. Moreover, blood was drawn from patients with acute appendicitis (9♀,11♂) and healthy volunteers (10♀,10♂) for determination...
Journal Articles
Subject Area:
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Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 41 (2): 731–741.
Published Online: 13 February 2017
... annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo-3 fluorescence, ROS formation from DCFDA dependent fluorescence, and ceramide abundance utilizing specific antibodies. Results: A 48 hours exposure of human erythrocytes to Camalexin significantly increased the percentage of annexin...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 41 (2): 543–554.
Published Online: 31 January 2017
... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Phosphatidylserine Cell volume Eryptosis Calcium 9-cis-retinoic acid Cellular Physiology Cell Physiol Biochem...
Journal Articles
Subject Area:
Further Areas
Morena Mischitelli, Mohamed Jemaàa, MyriamFezai Fezai, Mustafa Almasry, Florian Lang, Caterina Faggio
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 41 (2): 519–529.
Published Online: 30 January 2017
... was employed to estimate phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, reactive oxygen species (ROS) formation from DCFDA dependent fluorescence, and ceramide abundance utilizing specific antibodies. Results: A 48...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2017) 41 (1): 369–380.
Published Online: 26 January 2017
... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Cytosolic Ca 2+ concentration Phosphatidylserine translocation P-selectin Integrin Caspase Cell volume...
Journal Articles
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Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 40 (5): 1129–1140.
Published Online: 14 December 2016
...: Phosphatidylserine exposure at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, ROS formation from DCFDA dependent fluorescence, and ceramide abundance utilizing specific antibodies. Results: A 48 hours exposure of human erythrocytes...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 40 (3-4): 558–566.
Published Online: 25 November 2016
... rottlerin induces eryptosis and, if so, to test for the involvement of Ca 2+ entry and ceramide. Methods: Flow cytometry was employed to estimate phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, and ceramide...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 40 (3-4): 597–607.
Published Online: 25 November 2016
...-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, ROS formation from DCFDA dependent fluorescence, and ceramide abundance utilizing specific antibodies. Results: A 48 hours exposure of human erythrocytes to NSC-95397 significantly increased the percentage of annexin-V-binding...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 40 (1-2): 163–171.
Published Online: 18 November 2016
...: Phosphatidylserine exposure at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, and [Ca 2+ ] i from Fluo-3 fluorescence, as determined by flow cytometry. Results: A 48 hours exposure of human erythrocytes to Calyculin A (≥ 2.5 nM) significantly increased the percentage...
Journal Articles
Subject Area:
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Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 40 (1-2): 91–103.
Published Online: 18 November 2016
... induces eryptosis and, if so, to elucidate underlying cellular mechanisms. Methods: Phosphatidylserine abundance at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, ROS formation from DCFDA dependent fluorescence, and ceramide...
Journal Articles
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Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 39 (6): 2464–2476.
Published Online: 11 November 2016
... cytometry was employed to estimate phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, ROS formation from 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) dependent fluorescence, and ceramide abundance utilizing...
Journal Articles
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Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 39 (5): 1877–1890.
Published Online: 24 October 2016
... at the erythrocyte surface from annexin-V-binding, cell volume from forward scatter, and [Ca 2+ ] i from Fluo3 fluorescence. Results: Oxidative stress (60 min exposure to 0.3 mM tert-butylhydroperoxide), energy depletion (removal of glucose for 48 hours), and exposure to the Ca 2+ ionophore ionomycin (1 µM, 60 min...
Journal Articles
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 39 (4): 1638–1647.
Published Online: 15 September 2016
... study explored, whether Fascaplysin induces eryptosis and, if so, to shed light on the cellular mechanisms involved. Methods: Flow cytometry was employed to estimate phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3...
Journal Articles
Ca 2+ Entry, Oxidative Stress, Ceramide and Suicidal Erythrocyte Death Following Diosgenin Treatment
Subject Area:
Further Areas
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2016) 39 (4): 1626–1637.
Published Online: 15 September 2016
... annexin-V-binding, cell volume from forward scatter, [Ca 2+ ] i from Fluo3-fluorescence, ROS formation from DCF dependent fluorescence, and ceramide abundance utilizing specific antibodies. Hemolysis was quantified by determination of haemoglobin concentration in the supernatant. Results: A 48 hours...
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